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The map label for this gene is lasT [H]

Identifier: 119386406

GI number: 119386406

Start: 846979

End: 847719

Strand: Direct

Name: lasT [H]

Synonym: Pden_3698

Alternate gene names: 119386406

Gene position: 846979-847719 (Clockwise)

Preceding gene: 119386402

Following gene: 119386407

Centisome position: 48.96

GC content: 70.99

Gene sequence:

>741_bases
ATGGCCGCGCGCATGGAACCGGCGATCATTCTCATGCGCCCGCAGATGGGCGAAAACATCGGTGCGGCCGCGCGCGCCAT
GCTGAATTTCGGCCTGACGGACATGCGGCTGGTGGCGCCGCGCGACGGCTGGCCCAATCCCAGGGCCGTGGCCATGGCCT
CGGGTGCAGCCGGGCGCGTGCTGGACCGGGCACGGGTCTTTGCGACGCTGGCCGAGGCCATGGCGGACGTGGATTACGCC
TATGCCACCACCGCGCGGGGGCGCGAACTGACCAAGCCCGTTCTGACCCCTGCCACGGCCATGGCCGATGCCCGCGACCG
GGTGGCGCAGGGCGGCCGGGTCGCTGTGATCTTCGGTCCCGAGCGCGCGGGCCTGGAGAACGAGGACGTGGCGCGGGCCA
ATGCCATCATCACCGTGCCGGTGAACCCGGATTTCCCCTCGCTGAACCTGGCGCAGGCGGTGCTGCTGACCGGCTATGAA
TGGGCGCAGGACAGCCTGCCGCCCCAGCCCGCCCCGCATGGCCGCCGCCCGGATGGCGAGGCGCCCGCGACGCGGCTGGA
GATCGAGAAGCTCGCCGATCACTGGGAGGAGCGGCTGGCCGAGGCCGGTTTCTTCTTTCCGCCCGAAAAGGCGCCGGCGA
TGAAGCTGACGCTGCGCAACCTGTGGTCGCGACTGCCGCTGACCCGCGCCGATACGCGCATCTTCCACGGCATGCTGCGG
CAACTGATCCGCCGCGGCTGA

Upstream 100 bases:

>100_bases
TGCGGCGAATTGGGCTGGTCTGTCATGGCGCTGTCATCCTCGTGTTCCGCAAGCAGATAGCGCAGCGCTGCGCGCCTTGC
CAGTGGGTGCGCGGAAGGAT

Downstream 100 bases:

>100_bases
TTGATTTCCGGGTGCGCGCGGGCCAATGTCGCGCCCGATCGAGAAGGAAGCTGACATGGCAAGACGTCCCGTCTTTCAGG
AAGTCACCGAGACGACCCCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAARMEPAIILMRPQMGENIGAAARAMLNFGLTDMRLVAPRDGWPNPRAVAMASGAAGRVLDRARVFATLAEAMADVDYA
YATTARGRELTKPVLTPATAMADARDRVAQGGRVAVIFGPERAGLENEDVARANAIITVPVNPDFPSLNLAQAVLLTGYE
WAQDSLPPQPAPHGRRPDGEAPATRLEIEKLADHWEERLAEAGFFFPPEKAPAMKLTLRNLWSRLPLTRADTRIFHGMLR
QLIRRG

Sequences:

>Translated_246_residues
MAARMEPAIILMRPQMGENIGAAARAMLNFGLTDMRLVAPRDGWPNPRAVAMASGAAGRVLDRARVFATLAEAMADVDYA
YATTARGRELTKPVLTPATAMADARDRVAQGGRVAVIFGPERAGLENEDVARANAIITVPVNPDFPSLNLAQAVLLTGYE
WAQDSLPPQPAPHGRRPDGEAPATRLEIEKLADHWEERLAEAGFFFPPEKAPAMKLTLRNLWSRLPLTRADTRIFHGMLR
QLIRRG
>Mature_245_residues
AARMEPAIILMRPQMGENIGAAARAMLNFGLTDMRLVAPRDGWPNPRAVAMASGAAGRVLDRARVFATLAEAMADVDYAY
ATTARGRELTKPVLTPATAMADARDRVAQGGRVAVIFGPERAGLENEDVARANAIITVPVNPDFPSLNLAQAVLLTGYEW
AQDSLPPQPAPHGRRPDGEAPATRLEIEKLADHWEERLAEAGFFFPPEKAPAMKLTLRNLWSRLPLTRADTRIFHGMLRQ
LIRRG

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=184, Percent_Identity=39.6739130434783, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788881, Length=235, Percent_Identity=32.7659574468085, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26880; Mature: 26749

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARMEPAIILMRPQMGENIGAAARAMLNFGLTDMRLVAPRDGWPNPRAVAMASGAAGRV
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCHHHH
LDRARVFATLAEAMADVDYAYATTARGRELTKPVLTPATAMADARDRVAQGGRVAVIFGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECC
ERAGLENEDVARANAIITVPVNPDFPSLNLAQAVLLTGYEWAQDSLPPQPAPHGRRPDGE
CCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCC
APATRLEIEKLADHWEERLAEAGFFFPPEKAPAMKLTLRNLWSRLPLTRADTRIFHGMLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLIRRG
HHHHCC
>Mature Secondary Structure 
AARMEPAIILMRPQMGENIGAAARAMLNFGLTDMRLVAPRDGWPNPRAVAMASGAAGRV
CCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCHHHH
LDRARVFATLAEAMADVDYAYATTARGRELTKPVLTPATAMADARDRVAQGGRVAVIFGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECC
ERAGLENEDVARANAIITVPVNPDFPSLNLAQAVLLTGYEWAQDSLPPQPAPHGRRPDGE
CCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCC
APATRLEIEKLADHWEERLAEAGFFFPPEKAPAMKLTLRNLWSRLPLTRADTRIFHGMLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLIRRG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]