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The map label for this gene is nfdA [H]

Identifier: 119386218

GI number: 119386218

Start: 650698

End: 652557

Strand: Direct

Name: nfdA [H]

Synonym: Pden_3507

Alternate gene names: 119386218

Gene position: 650698-652557 (Clockwise)

Preceding gene: 119386217

Following gene: 119386219

Centisome position: 37.61

GC content: 67.8

Gene sequence:

>1860_bases
ATGCCGGAAAAAGACCTGATCATCGTCAATGCCAAGGTGACGACGCTCGACAAGCAGAACCCCTCGGCCGAGGCCGTGGC
CATCCGCGACGGCCGGTTCCTTGCCGTCGGCCCGGAAGCCGAGGTGCGGGCCGCCGCGCCCGAGGCGGACATCATCGACG
CGGGCGGGCGCCGCCTGATCCCCGGCCTGATCGACAGCCATACCCATGTGATCCGAGGCGGGCTGAACTACAATATGGAG
TTGCGCTGGGACGGCGTGCCCAGCCTGGCCGAGGCGATGCGGATGCTGAAGGACCAGGTCGCCCGCACGCCCGCGCCGCA
ATGGGTGCGCGTGGTCGGCGGCTTTACCGAGCACCAGTTCGCGGAAAGGCGCCTGCCGACGCTGGACGAGCTGAATGCCG
TGGCCCCCGACACGCCGGTCTTCATCCTGCATCTCTACGACCGGGCGTTGCTGAACGCCGCCGCGCTGCGGGTCGTGGGC
TATACGAAGGACACGCCCGATCCGCCGGGGGGCGAGATCGTCCGCGACGCCAAGGGCAATCCCACCGGCCTGCTGCTGGC
GCAGCCCAATGCGACCATCCTTTACTCGACGCTGGCCAGGGGGCCGAAGCTCGACCCGGAATACCAGCTCAATTCCTCGC
GCCATTTCATGCGCGAATTGAACGGGCTGGGCGTCACCTCGGTCATCGACGCTGGCGGTGGGTTCCAGAACTATCCCGAC
GATTACGAGATCGTCGAAAGGCTGCATGCCGAGGACCAGCTGACGGTGCGCATCAGCTACAACCTGTTCACGCAAAAGCC
CAAGGAGGAATTGGCCGATTTCTCGGGCTGGGTAAAGCAGGTCAGCCCCGGGCAGGGCGACGACACCTATCGCCACAACG
GTGCCGGCGAGATGCTGGTCTATTCCGCCGCCGATTTCGAGGATTTCCGGGTGGAGCGCCCCGAGATGCCGCCGAACATG
GAGGCCGACCTCGAACCCGTCATCCGCCTGCTGGCCGAGAACCGCTGGCCCTGGCGGTTGCACGCGACCTATGACGAGAC
GATCAGCCGCGCGCTGGACGTGTTCGAGAAGGTGAACCGCGACGTGCCCCTGCAAGGCATCAACTGGTTCTTCGACCATG
CCGAGACGATCAGCGAGCGCAGCATCGACCGGATCGCGGCGCTGGGCGGCGGCATCGCGGTGCAGCACCGGATGATGTAT
CAGGGCGAATATTTCATCGAACGCTACGGCGCGAAGGCGGCCGAGAACACGCCGCCGATCCGGCGGATGCTGGAGGCGGG
CCTGCCCGTCGGCGCCGGCACCGATGCGACGCGGGTGGCAAGCTACAACCCGTGGGTCTCGCTGTCCTGGCTGGTGACCT
CGCAGACCCTGGGCGGCACCCGGCTTTATCCGGCGCAAAACCGGCTGGACCGCGAGACGGCGCTGCGGCTCTGGACCGAG
GCGAACACCTGGTTCTCGAACGAGCAGGGCAAGAAGGGCCAGATCGAGGCGGGACAGCTGGCCGACCTGGCGCTGCTGTC
GGACGATTACTTCTCGGTCCCCGAGAACGAGATCGTGCACCTGCGCTCGGTCCTGACCGTGCTGGGCGGCAAGGTCGTGC
ATGGTGGGGACGAGTTCGGCCCTCTGGCCCCGGCGCTGCCCGCGCCGATGCCCGACTGGTCGCCGGTGGCGCGGTTCGGC
GGCTATTATCGCACGGCGGAGCTGCAGCAGGACCGGACCCGGTTCGCCGCCGCCTGCGGTTGCGGGCAGGGCTGTGCGGT
CCATGGCCATGACCACGCCGCCGCGCTTGGCGCCGATGTGCCGGCCTCGGACGCCCGGACCTTCTGGGGCAGCTTCGGCT
GCGGCTGCTGGGCCGTCTGA

Upstream 100 bases:

>100_bases
AGGAGCCGCAGATGTTCCGCCCCACACGCCGCCAGACCCTCATGGGGCCGCCGCTTCGGCCCTGCTGTCCTCAACCCGCA
CATTCGCGCAGGAGACGACC

Downstream 100 bases:

>100_bases
GCCGGACAGGATCGTAGCGGCGGGATGCGCGCATCCCGCCGTCCACGGATACCGATGCCTGGTGTCCATTCCCTTTTCGA
GAGGTATCGAGATGGCCGAA

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MPEKDLIIVNAKVTTLDKQNPSAEAVAIRDGRFLAVGPEAEVRAAAPEADIIDAGGRRLIPGLIDSHTHVIRGGLNYNME
LRWDGVPSLAEAMRMLKDQVARTPAPQWVRVVGGFTEHQFAERRLPTLDELNAVAPDTPVFILHLYDRALLNAAALRVVG
YTKDTPDPPGGEIVRDAKGNPTGLLLAQPNATILYSTLARGPKLDPEYQLNSSRHFMRELNGLGVTSVIDAGGGFQNYPD
DYEIVERLHAEDQLTVRISYNLFTQKPKEELADFSGWVKQVSPGQGDDTYRHNGAGEMLVYSAADFEDFRVERPEMPPNM
EADLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNRDVPLQGINWFFDHAETISERSIDRIAALGGGIAVQHRMMY
QGEYFIERYGAKAAENTPPIRRMLEAGLPVGAGTDATRVASYNPWVSLSWLVTSQTLGGTRLYPAQNRLDRETALRLWTE
ANTWFSNEQGKKGQIEAGQLADLALLSDDYFSVPENEIVHLRSVLTVLGGKVVHGGDEFGPLAPALPAPMPDWSPVARFG
GYYRTAELQQDRTRFAAACGCGQGCAVHGHDHAAALGADVPASDARTFWGSFGCGCWAV

Sequences:

>Translated_619_residues
MPEKDLIIVNAKVTTLDKQNPSAEAVAIRDGRFLAVGPEAEVRAAAPEADIIDAGGRRLIPGLIDSHTHVIRGGLNYNME
LRWDGVPSLAEAMRMLKDQVARTPAPQWVRVVGGFTEHQFAERRLPTLDELNAVAPDTPVFILHLYDRALLNAAALRVVG
YTKDTPDPPGGEIVRDAKGNPTGLLLAQPNATILYSTLARGPKLDPEYQLNSSRHFMRELNGLGVTSVIDAGGGFQNYPD
DYEIVERLHAEDQLTVRISYNLFTQKPKEELADFSGWVKQVSPGQGDDTYRHNGAGEMLVYSAADFEDFRVERPEMPPNM
EADLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNRDVPLQGINWFFDHAETISERSIDRIAALGGGIAVQHRMMY
QGEYFIERYGAKAAENTPPIRRMLEAGLPVGAGTDATRVASYNPWVSLSWLVTSQTLGGTRLYPAQNRLDRETALRLWTE
ANTWFSNEQGKKGQIEAGQLADLALLSDDYFSVPENEIVHLRSVLTVLGGKVVHGGDEFGPLAPALPAPMPDWSPVARFG
GYYRTAELQQDRTRFAAACGCGQGCAVHGHDHAAALGADVPASDARTFWGSFGCGCWAV
>Mature_618_residues
PEKDLIIVNAKVTTLDKQNPSAEAVAIRDGRFLAVGPEAEVRAAAPEADIIDAGGRRLIPGLIDSHTHVIRGGLNYNMEL
RWDGVPSLAEAMRMLKDQVARTPAPQWVRVVGGFTEHQFAERRLPTLDELNAVAPDTPVFILHLYDRALLNAAALRVVGY
TKDTPDPPGGEIVRDAKGNPTGLLLAQPNATILYSTLARGPKLDPEYQLNSSRHFMRELNGLGVTSVIDAGGGFQNYPDD
YEIVERLHAEDQLTVRISYNLFTQKPKEELADFSGWVKQVSPGQGDDTYRHNGAGEMLVYSAADFEDFRVERPEMPPNME
ADLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNRDVPLQGINWFFDHAETISERSIDRIAALGGGIAVQHRMMYQ
GEYFIERYGAKAAENTPPIRRMLEAGLPVGAGTDATRVASYNPWVSLSWLVTSQTLGGTRLYPAQNRLDRETALRLWTEA
NTWFSNEQGKKGQIEAGQLADLALLSDDYFSVPENEIVHLRSVLTVLGGKVVHGGDEFGPLAPALPAPMPDWSPVARFGG
YYRTAELQQDRTRFAAACGCGQGCAVHGHDHAAALGADVPASDARTFWGSFGCGCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68179; Mature: 68047

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKDLIIVNAKVTTLDKQNPSAEAVAIRDGRFLAVGPEAEVRAAAPEADIIDAGGRRLI
CCCCCEEEEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEECCCCCCEECCCCCEEC
PGLIDSHTHVIRGGLNYNMELRWDGVPSLAEAMRMLKDQVARTPAPQWVRVVGGFTEHQF
CHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
AERRLPTLDELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPDPPGGEIVRDAKGN
HHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCCC
PTGLLLAQPNATILYSTLARGPKLDPEYQLNSSRHFMRELNGLGVTSVIDAGGGFQNYPD
CCEEEEECCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEECCCCCCCCCC
DYEIVERLHAEDQLTVRISYNLFTQKPKEELADFSGWVKQVSPGQGDDTYRHNGAGEMLV
HHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEE
YSAADFEDFRVERPEMPPNMEADLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNR
EECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC
DVPLQGINWFFDHAETISERSIDRIAALGGGIAVQHRMMYQGEYFIERYGAKAAENTPPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
RRMLEAGLPVGAGTDATRVASYNPWVSLSWLVTSQTLGGTRLYPAQNRLDRETALRLWTE
HHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCEECCCHHHCCHHHHHHHHCC
ANTWFSNEQGKKGQIEAGQLADLALLSDDYFSVPENEIVHLRSVLTVLGGKVVHGGDEFG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
PLAPALPAPMPDWSPVARFGGYYRTAELQQDRTRFAAACGCGQGCAVHGHDHAAALGADV
CCCCCCCCCCCCCCHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCC
PASDARTFWGSFGCGCWAV
CCCCHHHHHCCCCCCEECC
>Mature Secondary Structure 
PEKDLIIVNAKVTTLDKQNPSAEAVAIRDGRFLAVGPEAEVRAAAPEADIIDAGGRRLI
CCCCEEEEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEECCCCCCEECCCCCEEC
PGLIDSHTHVIRGGLNYNMELRWDGVPSLAEAMRMLKDQVARTPAPQWVRVVGGFTEHQF
CHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
AERRLPTLDELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPDPPGGEIVRDAKGN
HHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCCC
PTGLLLAQPNATILYSTLARGPKLDPEYQLNSSRHFMRELNGLGVTSVIDAGGGFQNYPD
CCEEEEECCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEECCCCCCCCCC
DYEIVERLHAEDQLTVRISYNLFTQKPKEELADFSGWVKQVSPGQGDDTYRHNGAGEMLV
HHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEE
YSAADFEDFRVERPEMPPNMEADLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNR
EECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC
DVPLQGINWFFDHAETISERSIDRIAALGGGIAVQHRMMYQGEYFIERYGAKAAENTPPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
RRMLEAGLPVGAGTDATRVASYNPWVSLSWLVTSQTLGGTRLYPAQNRLDRETALRLWTE
HHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCEECCCHHHCCHHHHHHHHCC
ANTWFSNEQGKKGQIEAGQLADLALLSDDYFSVPENEIVHLRSVLTVLGGKVVHGGDEFG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
PLAPALPAPMPDWSPVARFGGYYRTAELQQDRTRFAAACGCGQGCAVHGHDHAAALGADV
CCCCCCCCCCCCCCHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCC
PASDARTFWGSFGCGCWAV
CCCCHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA