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The map label for this gene is yiaO [H]

Identifier: 119386191

GI number: 119386191

Start: 626754

End: 627746

Strand: Direct

Name: yiaO [H]

Synonym: Pden_3480

Alternate gene names: 119386191

Gene position: 626754-627746 (Clockwise)

Preceding gene: 119386189

Following gene: 119386192

Centisome position: 36.23

GC content: 63.44

Gene sequence:

>993_bases
ATGATGAAGACTGCCGCTGCCGTCCTGGTGGGAAGCCTGCTGTCGAGTGCCGCCTGGGCCGACCCCGTCACGCTGCGCCT
GGGCCATGCCGTATTCGAGCAGCATCCGATCCACGACACCGCCCTCCGCTTCAAGGAAGCGGTCGAGCGGCTGTCGGATG
GCGATATCAAGATCGACATTTTCCCCTCGCGCCAGCTTGGCGACGTGAAGGAGTTGATGGAGGGCGTGCAGTTCGGCACC
GTCGACATGACGGTGAACTCGACCTCGGCCTATTCGACGCTGGAGCCCTCGATCGACGCCTTCCAGCTGCCGGGCCTGAT
CCCCGATTACGAAGGGTTCGCGGCCATGGCGAAGACGCCCGAGGCGCGGGCGATCATGGACAGGCTGGGAAACCACATGA
TGGTCGCCCTGGGGGTCTATGACGGCGGGCAACGCCATTTCCTGACCGTCGGGGCGCCGGTCGAAAGCCTGGCCGACATG
CATCACAAGAAGACCCGCGTCGCGCCCAACTGGATGTTTCTGGATGCGTGGCAGGCGGTGGGCGTGAACCCCACGCCGAT
GGCCTATGGCGAGGTCTATTCGGCGCTGGAAACCGGCACGCTGGATGCGGTCGAGATCAACCTGACCTCGATCGAGAGCG
AGAAATATTACGAGATCGCCAAGGGCGTGTCGCTGACAGGCCATTACTTCTGGCCCAGCTTCCTGCTGGTCAACCAGATG
AAGTTCAACTCGCTGACCCCCGAGCAGCAGGAGATCATGCGCCAGGCCGCCGACGAGATCGTCGAGCCGCAGGTGATGGC
GGTCAAGGATCTGGACGAGCAGATCAAGGCCCATCTGGCCGAACTGAACATCCCGGTCGTGACGCCCTCGGATGCGTTCA
GGGCCGAATTGCAGGCCGCCTTCGCCCCGGTGGTCGAGAAATACGAGGCCGCCGATCCGCTGATCGCCGATTTCGCGGCT
GCGGCGAAAAAGCTGGCTCCGGCCGCTCAATAA

Upstream 100 bases:

>100_bases
CCCCATGAATTTGCTCCTCCTCTCTGTGTTGACGACTTTGCCGTGGCGTGTCAATATGAGAACAGTGTTCCCATATAGAG
AACACAGGAGGAGAGAAAAC

Downstream 100 bases:

>100_bases
CGCACAGGCGCGCGGGCCGGTCCCGCGCCTTCCGCCTGCGTTCTCAACGGAGAAACCCATGTCCAAGCTCTGGCGCGCCT
ATGTCGCCGCCGTGGAAGCA

Product: TRAP dicarboxylate transporter, DctP subunit

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MMKTAAAVLVGSLLSSAAWADPVTLRLGHAVFEQHPIHDTALRFKEAVERLSDGDIKIDIFPSRQLGDVKELMEGVQFGT
VDMTVNSTSAYSTLEPSIDAFQLPGLIPDYEGFAAMAKTPEARAIMDRLGNHMMVALGVYDGGQRHFLTVGAPVESLADM
HHKKTRVAPNWMFLDAWQAVGVNPTPMAYGEVYSALETGTLDAVEINLTSIESEKYYEIAKGVSLTGHYFWPSFLLVNQM
KFNSLTPEQQEIMRQAADEIVEPQVMAVKDLDEQIKAHLAELNIPVVTPSDAFRAELQAAFAPVVEKYEAADPLIADFAA
AAKKLAPAAQ

Sequences:

>Translated_330_residues
MMKTAAAVLVGSLLSSAAWADPVTLRLGHAVFEQHPIHDTALRFKEAVERLSDGDIKIDIFPSRQLGDVKELMEGVQFGT
VDMTVNSTSAYSTLEPSIDAFQLPGLIPDYEGFAAMAKTPEARAIMDRLGNHMMVALGVYDGGQRHFLTVGAPVESLADM
HHKKTRVAPNWMFLDAWQAVGVNPTPMAYGEVYSALETGTLDAVEINLTSIESEKYYEIAKGVSLTGHYFWPSFLLVNQM
KFNSLTPEQQEIMRQAADEIVEPQVMAVKDLDEQIKAHLAELNIPVVTPSDAFRAELQAAFAPVVEKYEAADPLIADFAA
AAKKLAPAAQ
>Mature_330_residues
MMKTAAAVLVGSLLSSAAWADPVTLRLGHAVFEQHPIHDTALRFKEAVERLSDGDIKIDIFPSRQLGDVKELMEGVQFGT
VDMTVNSTSAYSTLEPSIDAFQLPGLIPDYEGFAAMAKTPEARAIMDRLGNHMMVALGVYDGGQRHFLTVGAPVESLADM
HHKKTRVAPNWMFLDAWQAVGVNPTPMAYGEVYSALETGTLDAVEINLTSIESEKYYEIAKGVSLTGHYFWPSFLLVNQM
KFNSLTPEQQEIMRQAADEIVEPQVMAVKDLDEQIKAHLAELNIPVVTPSDAFRAELQAAFAPVVEKYEAADPLIADFAA
AAKKLAPAAQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=271, Percent_Identity=29.520295202952, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36065; Mature: 36065

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTAAAVLVGSLLSSAAWADPVTLRLGHAVFEQHPIHDTALRFKEAVERLSDGDIKIDI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEE
FPSRQLGDVKELMEGVQFGTVDMTVNSTSAYSTLEPSIDAFQLPGLIPDYEGFAAMAKTP
ECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHCCC
EARAIMDRLGNHMMVALGVYDGGQRHFLTVGAPVESLADMHHKKTRVAPNWMFLDAWQAV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECHHHC
GVNPTPMAYGEVYSALETGTLDAVEINLTSIESEKYYEIAKGVSLTGHYFWPSFLLVNQM
CCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCEEEECHHHHHHHHHHHH
KFNSLTPEQQEIMRQAADEIVEPQVMAVKDLDEQIKAHLAELNIPVVTPSDAFRAELQAA
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHH
FAPVVEKYEAADPLIADFAAAAKKLAPAAQ
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMKTAAAVLVGSLLSSAAWADPVTLRLGHAVFEQHPIHDTALRFKEAVERLSDGDIKIDI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEE
FPSRQLGDVKELMEGVQFGTVDMTVNSTSAYSTLEPSIDAFQLPGLIPDYEGFAAMAKTP
ECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHCCC
EARAIMDRLGNHMMVALGVYDGGQRHFLTVGAPVESLADMHHKKTRVAPNWMFLDAWQAV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECHHHC
GVNPTPMAYGEVYSALETGTLDAVEINLTSIESEKYYEIAKGVSLTGHYFWPSFLLVNQM
CCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCEEEECHHHHHHHHHHHH
KFNSLTPEQQEIMRQAADEIVEPQVMAVKDLDEQIKAHLAELNIPVVTPSDAFRAELQAA
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHH
FAPVVEKYEAADPLIADFAAAAKKLAPAAQ
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]