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The map label for this gene is yneJ [H]

Identifier: 119386135

GI number: 119386135

Start: 566180

End: 567067

Strand: Direct

Name: yneJ [H]

Synonym: Pden_3423

Alternate gene names: 119386135

Gene position: 566180-567067 (Clockwise)

Preceding gene: 119386127

Following gene: 119386139

Centisome position: 32.73

GC content: 70.72

Gene sequence:

>888_bases
ATGGATATTCGTTTTCTGGAAAGCTTCGTGACCGTGGCCGAATGCGGCTCGATCGCCGAGGCGGCGCGGCGGCTGAACCT
GACGCCCGCCGCGCTGGCCCAGCGGCTGCGTGCGCTGGAGCAGGATCTGGGCCATTCGCTGGTCAGCCGGGTGGGGCGCA
CGGTGCGGCCGACCGCCTCGGGCCTGGCGGTGCTGCGCCATGCCGGGGCACTGATCGAGGGCGCGCGCGACCTGCGCGCC
ATCGCCGCCAACGACCAGCCCGAGGGACAGTTGCGCCTGGGCGCCACGGCGACCGCGCTGACCGGCCTGCTGCCCGCGAT
CATCGAGACGCTGGGCGCGCGCCATCCGCGGATCGAGCTGTTCATCCGGCCCGGCGCCTCGGTCGATCTTTATCACGGTG
TCGTTGCCGGCGACCTGGATGCCGCGGTGATCGTGCGGCCGCAATTTCCGGTGCCCAAGGCCACGGGGTGGCTGACCCTG
CGCGAAGAGCCTCTGGTGCTCATAGCGCGTGCCGGCATGGCGCTGGACGATCCGTTCGACGTGATCCGGCGTCACCCCTT
CATCCGCTATGATCGCAACCAATGGGGCGGCCAGATCGTGGACCGTTTCCTGCGCCAACAGGGGCTGAAGGTGCGCGAAT
GGCTGGAGCTGGACGCATTGGACGCCATCGCCGCGCTGGTCAGCCGGGGGTTGGGCATCGCCATCGTGCCCGATTGGGCG
CCGCCATGGCCCGAGGGGCTGCGGCTGGAAAAGCTGCCGCTCGCGGGTGCGGGCTCGCGCCAGACCGGCGTGCTGTGGAA
CCGTTCCGGCGCGCGTATCGCGGCGGTGCGCGCATTCGTGGATGTCTGTGGCGGTGGACAGGCCCCGACGCAGGGACGGG
GCCTGTAG

Upstream 100 bases:

>100_bases
GCGTTGGGGGCGGGCGGCGTTTCCCTGGGCGCATCTTACGCCAGTCGGAGCGCAAGAAAATCTGGCTTATTCTTGCTTCA
GAAGATAGAAAAACCATCTT

Downstream 100 bases:

>100_bases
GGATCAGGCGGCCAGCCGGGTTTCCAGCCGGGCCATCAGCCGCTCGACCTCGGCGATATCGGTGGCACTCAGCCTGGGCC
CCGGTGCGCGCGTGGCGGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDIRFLESFVTVAECGSIAEAARRLNLTPAALAQRLRALEQDLGHSLVSRVGRTVRPTASGLAVLRHAGALIEGARDLRA
IAANDQPEGQLRLGATATALTGLLPAIIETLGARHPRIELFIRPGASVDLYHGVVAGDLDAAVIVRPQFPVPKATGWLTL
REEPLVLIARAGMALDDPFDVIRRHPFIRYDRNQWGGQIVDRFLRQQGLKVREWLELDALDAIAALVSRGLGIAIVPDWA
PPWPEGLRLEKLPLAGAGSRQTGVLWNRSGARIAAVRAFVDVCGGGQAPTQGRGL

Sequences:

>Translated_295_residues
MDIRFLESFVTVAECGSIAEAARRLNLTPAALAQRLRALEQDLGHSLVSRVGRTVRPTASGLAVLRHAGALIEGARDLRA
IAANDQPEGQLRLGATATALTGLLPAIIETLGARHPRIELFIRPGASVDLYHGVVAGDLDAAVIVRPQFPVPKATGWLTL
REEPLVLIARAGMALDDPFDVIRRHPFIRYDRNQWGGQIVDRFLRQQGLKVREWLELDALDAIAALVSRGLGIAIVPDWA
PPWPEGLRLEKLPLAGAGSRQTGVLWNRSGARIAAVRAFVDVCGGGQAPTQGRGL
>Mature_295_residues
MDIRFLESFVTVAECGSIAEAARRLNLTPAALAQRLRALEQDLGHSLVSRVGRTVRPTASGLAVLRHAGALIEGARDLRA
IAANDQPEGQLRLGATATALTGLLPAIIETLGARHPRIELFIRPGASVDLYHGVVAGDLDAAVIVRPQFPVPKATGWLTL
REEPLVLIARAGMALDDPFDVIRRHPFIRYDRNQWGGQIVDRFLRQQGLKVREWLELDALDAIAALVSRGLGIAIVPDWA
PPWPEGLRLEKLPLAGAGSRQTGVLWNRSGARIAAVRAFVDVCGGGQAPTQGRGL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=27.6450511945392, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=27.4193548387097, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082132, Length=288, Percent_Identity=25.3472222222222, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=28.9772727272727, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI157672245, Length=303, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=257, Percent_Identity=26.4591439688716, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788297, Length=257, Percent_Identity=23.7354085603113, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788748, Length=297, Percent_Identity=24.5791245791246, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=23.7931034482759, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=146, Percent_Identity=28.0821917808219, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31779; Mature: 31779

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRFLESFVTVAECGSIAEAARRLNLTPAALAQRLRALEQDLGHSLVSRVGRTVRPTAS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GLAVLRHAGALIEGARDLRAIAANDQPEGQLRLGATATALTGLLPAIIETLGARHPRIEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCEEEE
FIRPGASVDLYHGVVAGDLDAAVIVRPQFPVPKATGWLTLREEPLVLIARAGMALDDPFD
EECCCCCEEHHCCEEECCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHH
VIRRHPFIRYDRNQWGGQIVDRFLRQQGLKVREWLELDALDAIAALVSRGLGIAIVPDWA
HHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PPWPEGLRLEKLPLAGAGSRQTGVLWNRSGARIAAVRAFVDVCGGGQAPTQGRGL
CCCCCCCEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDIRFLESFVTVAECGSIAEAARRLNLTPAALAQRLRALEQDLGHSLVSRVGRTVRPTAS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GLAVLRHAGALIEGARDLRAIAANDQPEGQLRLGATATALTGLLPAIIETLGARHPRIEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCEEEE
FIRPGASVDLYHGVVAGDLDAAVIVRPQFPVPKATGWLTLREEPLVLIARAGMALDDPFD
EECCCCCEEHHCCEEECCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHH
VIRRHPFIRYDRNQWGGQIVDRFLRQQGLKVREWLELDALDAIAALVSRGLGIAIVPDWA
HHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PPWPEGLRLEKLPLAGAGSRQTGVLWNRSGARIAAVRAFVDVCGGGQAPTQGRGL
CCCCCCCEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]