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The map label for this gene is yneJ [H]

Identifier: 119386127

GI number: 119386127

Start: 559011

End: 560171

Strand: Direct

Name: yneJ [H]

Synonym: Pden_3415

Alternate gene names: 119386127

Gene position: 559011-560171 (Clockwise)

Preceding gene: 119386121

Following gene: 119386135

Centisome position: 32.31

GC content: 64.86

Gene sequence:

>1161_bases
ATGCAGCGCCAGGTTGCACTCTCGGAATCGAGCGCGCTGCTCTCAGGGGCCCTGCCCAAGCCGCCGGAATGCAGAACGGC
CGGAGGCGCCTCCGGTCGTTGCGATTTCAAGCCTCTTCCTCCTGGAAGGCCTCTTTGCGCTTCTTGCGGAAGCGAACACG
GTTTCATCGATGCGCCCCATGCGTGCCAGAATGCCGACCGGATCGGCCATGCGCACGACCTCGATCAGACCATCGCCGCT
GACATAGGGCGGAACGTAGACCGGAACATAACCCGCTCGCGCCAGCTAAAGACGCTTCTCGCCATTGTGGAAACCGGCAG
CTTCGCCCGTGCCGCGAACCGCGTTGCGCTGACGCCCTCGGCGGTCAGCCAGCAGATCCAGGCCCTTGAAAACGAAGTCG
GAGTTTCGCTTTTCAACCGCGAGAACCGCCCGCCGACGCTGACCGCGGCGGGGCAGCAGATGGTCGAGGCGGCGGCAGAG
CTCGTCAGGGCGGCAGAAAACGCCATCGACGCGATTTCAGGCCGCCGGATCGTGGGAAAGTTTGTCATCGGCTCGGTCCG
CACCAGCGCGATAGGGCTGCTGCCGCAAGCGGTATCGCGCGTCGTGGCGAATCATCCGGGGCTGAAGATCCGGCTTCTGG
TCGGGAACTCGGAAAATCTGGTCCAGGATGTCTATGCCGGCAGGCTGGACGCCGCGATCATCGCCGAGAACAGCCTGTCG
TCGCGCGATCTCGACTGGTCGCCCTTTATCCGCGAGCCGCTGTTTCTGATCGCGCCCCCGGGAACCCCGGTCGTGGATGT
CCGGCAGGCGTTGTCGGCGTTTCCCTTCGTGCGGTTCCGCAGCAACGTGCCCTTGGCGCGGCTGATCGAAACCGAGCTGG
GCCGGATGAACCTGCCGCTGAACGACATCGCCGAGATGGACACGATTTCGGCGGTGACCGCCTGTGTCGAAAACGGGCTG
GGGGTGTCGATCGTGCCGCGGATCGCCGCGATCGAGCGCGGGGTGGACCTGGTCATGTTGCCTTTCGGAGATCCGCCGGT
CTTTCGCCAGATCGGGCTGCTGCAACGCCCGAAAGGGCCTCGTGCCGGGCTGATCGCCGAATTGCATGAGCAACTGGCCC
AGGTCAGCGGCGAATATGGGGTGCCCAGGCCCTTGTCGTGA

Upstream 100 bases:

>100_bases
TTCCTGGAGGGCCCGGAACCGGGCGAGGCGTCAGCCGGAGAACCCGGCCCCCGCCCGCGAACATGCCGGGCCGAACCGTC
CTCATGGGGAAGTCTAGCAG

Downstream 100 bases:

>100_bases
TGGGTTGGGCGCTCTCGCAGGGCCCCGCACCCGGACGCTCCGTCACCCGGTTTCGCGGCGGAACAGCGATGGGGATGCCC
GCTGGGGCTGGTCCCTGATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQRQVALSESSALLSGALPKPPECRTAGGASGRCDFKPLPPGRPLCASCGSEHGFIDAPHACQNADRIGHAHDLDQTIAA
DIGRNVDRNITRSRQLKTLLAIVETGSFARAANRVALTPSAVSQQIQALENEVGVSLFNRENRPPTLTAAGQQMVEAAAE
LVRAAENAIDAISGRRIVGKFVIGSVRTSAIGLLPQAVSRVVANHPGLKIRLLVGNSENLVQDVYAGRLDAAIIAENSLS
SRDLDWSPFIREPLFLIAPPGTPVVDVRQALSAFPFVRFRSNVPLARLIETELGRMNLPLNDIAEMDTISAVTACVENGL
GVSIVPRIAAIERGVDLVMLPFGDPPVFRQIGLLQRPKGPRAGLIAELHEQLAQVSGEYGVPRPLS

Sequences:

>Translated_386_residues
MQRQVALSESSALLSGALPKPPECRTAGGASGRCDFKPLPPGRPLCASCGSEHGFIDAPHACQNADRIGHAHDLDQTIAA
DIGRNVDRNITRSRQLKTLLAIVETGSFARAANRVALTPSAVSQQIQALENEVGVSLFNRENRPPTLTAAGQQMVEAAAE
LVRAAENAIDAISGRRIVGKFVIGSVRTSAIGLLPQAVSRVVANHPGLKIRLLVGNSENLVQDVYAGRLDAAIIAENSLS
SRDLDWSPFIREPLFLIAPPGTPVVDVRQALSAFPFVRFRSNVPLARLIETELGRMNLPLNDIAEMDTISAVTACVENGL
GVSIVPRIAAIERGVDLVMLPFGDPPVFRQIGLLQRPKGPRAGLIAELHEQLAQVSGEYGVPRPLS
>Mature_386_residues
MQRQVALSESSALLSGALPKPPECRTAGGASGRCDFKPLPPGRPLCASCGSEHGFIDAPHACQNADRIGHAHDLDQTIAA
DIGRNVDRNITRSRQLKTLLAIVETGSFARAANRVALTPSAVSQQIQALENEVGVSLFNRENRPPTLTAAGQQMVEAAAE
LVRAAENAIDAISGRRIVGKFVIGSVRTSAIGLLPQAVSRVVANHPGLKIRLLVGNSENLVQDVYAGRLDAAIIAENSLS
SRDLDWSPFIREPLFLIAPPGTPVVDVRQALSAFPFVRFRSNVPLARLIETELGRMNLPLNDIAEMDTISAVTACVENGL
GVSIVPRIAAIERGVDLVMLPFGDPPVFRQIGLLQRPKGPRAGLIAELHEQLAQVSGEYGVPRPLS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=233, Percent_Identity=27.8969957081545, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693105, Length=268, Percent_Identity=25.3731343283582, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788748, Length=264, Percent_Identity=26.8939393939394, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=29.9180327868852, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082132, Length=267, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788887, Length=242, Percent_Identity=27.6859504132231, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790399, Length=302, Percent_Identity=27.4834437086093, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=243, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI48994962, Length=144, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=245, Percent_Identity=27.7551020408163, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788626, Length=234, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=30.2788844621514, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=297, Percent_Identity=23.5690235690236, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=134, Percent_Identity=31.3432835820896, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789639, Length=167, Percent_Identity=26.9461077844311, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=280, Percent_Identity=26.7857142857143, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=265, Percent_Identity=22.2641509433962, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789506, Length=243, Percent_Identity=23.0452674897119, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 41165; Mature: 41165

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQVALSESSALLSGALPKPPECRTAGGASGRCDFKPLPPGRPLCASCGSEHGFIDAPH
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCH
ACQNADRIGHAHDLDQTIAADIGRNVDRNITRSRQLKTLLAIVETGSFARAANRVALTPS
HHCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEECHH
AVSQQIQALENEVGVSLFNRENRPPTLTAAGQQMVEAAAELVRAAENAIDAISGRRIVGK
HHHHHHHHHHHHHCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FVIGSVRTSAIGLLPQAVSRVVANHPGLKIRLLVGNSENLVQDVYAGRLDAAIIAENSLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCC
SRDLDWSPFIREPLFLIAPPGTPVVDVRQALSAFPFVRFRSNVPLARLIETELGRMNLPL
CCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCH
NDIAEMDTISAVTACVENGLGVSIVPRIAAIERGVDLVMLPFGDPPVFRQIGLLQRPKGP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCC
RAGLIAELHEQLAQVSGEYGVPRPLS
CCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQRQVALSESSALLSGALPKPPECRTAGGASGRCDFKPLPPGRPLCASCGSEHGFIDAPH
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCH
ACQNADRIGHAHDLDQTIAADIGRNVDRNITRSRQLKTLLAIVETGSFARAANRVALTPS
HHCCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEECHH
AVSQQIQALENEVGVSLFNRENRPPTLTAAGQQMVEAAAELVRAAENAIDAISGRRIVGK
HHHHHHHHHHHHHCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FVIGSVRTSAIGLLPQAVSRVVANHPGLKIRLLVGNSENLVQDVYAGRLDAAIIAENSLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCC
SRDLDWSPFIREPLFLIAPPGTPVVDVRQALSAFPFVRFRSNVPLARLIETELGRMNLPL
CCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCH
NDIAEMDTISAVTACVENGLGVSIVPRIAAIERGVDLVMLPFGDPPVFRQIGLLQRPKGP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCC
RAGLIAELHEQLAQVSGEYGVPRPLS
CCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]