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The map label for this gene is rlmN

Identifier: 119357341

GI number: 119357341

Start: 1748156

End: 1749247

Strand: Direct

Name: rlmN

Synonym: Cpha266_1539

Alternate gene names: 119357341

Gene position: 1748156-1749247 (Clockwise)

Preceding gene: 119357335

Following gene: 119357342

Centisome position: 55.78

GC content: 43.96

Gene sequence:

>1092_bases
ATGCAAACAAGACTGTCCAACATAAAACATCTGTCAAGACAAGAGCTCCGGCAAGCTATTGCAAATCTTGGGGAACCAGC
ATACAGAACCCGCCAGATTCATCAATGGATCTTCAGCCATCGAGCAGCCACATTTGAGGAAATGACCACAATAAGTCTGG
AATTAAGAAATAAACTTGCCGATCAATTCCGGATTGGCTTCCCCATACTTGCCGACTGTCAACAGGATGGCAGTGCGAAT
GACCCCTTCTCTACAGTTAAACTGCTCCTCGAACTGGATGACAATGAGAAAATTGAAACCGTACTCATCCCTTCCGAAAA
TCGGATGACTGCCTGCGTTTCCTCACAGGTCGGCTGTCCTTTGCAATGCAGATTCTGTGCATCCGGACAGACGGGCTTCA
AAAGAAATCTTTCGGCTGACGAGATCATCGATCAGGTGTTTTCACTCAATGATTTCATCCGGACAAAACATGAGAGCAAC
GAAATCACCAACATCGTCTTTATGGGAATGGGTGAACCGTTACTGAATTTCGAAAACCTGAAAGAGTCCATCGAGGTTCT
TTCAGATCAAAGCTATAAATTCAATCTTCCCCAACGAAAAATCACGATTTCAACAGTTGGCATTATCCCAGGCATCAATG
AGCTTGGGAAATCAGGCCTTAAAACGAAACTTGCAATTTCTCTGCATAGCGCCTCGCAAGAAACACGAGAATCACTTATA
CCTGTTGCCAGCGAATTCTCTCTGACCCAACTCCGAAAAACTCTTTCAGAATACACCTCTCAAACAGGAGAACCGGTTAC
GCTCGTTTATATGCTGCTCAAGGGAATCAATGACTCAGTTGAGGATGCCCGACTTCTTGTAAAATTCTCAAGAAGCTTTT
TATGCAAAATTAATTTGATTGATTATAATTCAATCATTAACATGAAGTTTAAGCCCGTTTTTAACGAGACAAAAGACATG
TTTATTCAGCACATCCTTGACGCAGGGATTCACGTCACCGTCCGCAAAAGCCATGGGGCATCGATCAATGCTGCTTGCGG
ACAACTTGCGGCAAAGGGAACGCAGAAAGCCGAGAATCGCAACAATCTCTAA

Upstream 100 bases:

>100_bases
TTTTCCTGAAAAGCAGCCCATGTATGGTTCAACAAATTTCGTTTTATTAGGTAATCTGAAAAAGAGTACTTTGTTCGATT
TGATCACCTGACTTTATCCA

Downstream 100 bases:

>100_bases
TCAGCATCATCAAATTATGGATTTGACAGATTTCATTCCTTTCAAAAACGAATTTGCCAAAGCTTATCATGGCTTGACAG
GCAACGCAACGCACACGTCG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQTRLSNIKHLSRQELRQAIANLGEPAYRTRQIHQWIFSHRAATFEEMTTISLELRNKLADQFRIGFPILADCQQDGSAN
DPFSTVKLLLELDDNEKIETVLIPSENRMTACVSSQVGCPLQCRFCASGQTGFKRNLSADEIIDQVFSLNDFIRTKHESN
EITNIVFMGMGEPLLNFENLKESIEVLSDQSYKFNLPQRKITISTVGIIPGINELGKSGLKTKLAISLHSASQETRESLI
PVASEFSLTQLRKTLSEYTSQTGEPVTLVYMLLKGINDSVEDARLLVKFSRSFLCKINLIDYNSIINMKFKPVFNETKDM
FIQHILDAGIHVTVRKSHGASINAACGQLAAKGTQKAENRNNL

Sequences:

>Translated_363_residues
MQTRLSNIKHLSRQELRQAIANLGEPAYRTRQIHQWIFSHRAATFEEMTTISLELRNKLADQFRIGFPILADCQQDGSAN
DPFSTVKLLLELDDNEKIETVLIPSENRMTACVSSQVGCPLQCRFCASGQTGFKRNLSADEIIDQVFSLNDFIRTKHESN
EITNIVFMGMGEPLLNFENLKESIEVLSDQSYKFNLPQRKITISTVGIIPGINELGKSGLKTKLAISLHSASQETRESLI
PVASEFSLTQLRKTLSEYTSQTGEPVTLVYMLLKGINDSVEDARLLVKFSRSFLCKINLIDYNSIINMKFKPVFNETKDM
FIQHILDAGIHVTVRKSHGASINAACGQLAAKGTQKAENRNNL
>Mature_363_residues
MQTRLSNIKHLSRQELRQAIANLGEPAYRTRQIHQWIFSHRAATFEEMTTISLELRNKLADQFRIGFPILADCQQDGSAN
DPFSTVKLLLELDDNEKIETVLIPSENRMTACVSSQVGCPLQCRFCASGQTGFKRNLSADEIIDQVFSLNDFIRTKHESN
EITNIVFMGMGEPLLNFENLKESIEVLSDQSYKFNLPQRKITISTVGIIPGINELGKSGLKTKLAISLHSASQETRESLI
PVASEFSLTQLRKTLSEYTSQTGEPVTLVYMLLKGINDSVEDARLLVKFSRSFLCKINLIDYNSIINMKFKPVFNETKDM
FIQHILDAGIHVTVRKSHGASINAACGQLAAKGTQKAENRNNL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=36.1823361823362, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CHLPD (A1BGN4)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911985.1
- ProteinModelPortal:   A1BGN4
- STRING:   A1BGN4
- GeneID:   4569124
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1539
- NMPDR:   fig|290317.7.peg.1621
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   SSQCGCG
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40745; Mature: 40745

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRLSNIKHLSRQELRQAIANLGEPAYRTRQIHQWIFSHRAATFEEMTTISLELRNKLA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQFRIGFPILADCQQDGSANDPFSTVKLLLELDDNEKIETVLIPSENRMTACVSSQVGCP
HHHHCCCCEEECCCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCCC
LQCRFCASGQTGFKRNLSADEIIDQVFSLNDFIRTKHESNEITNIVFMGMGEPLLNFENL
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHCCHHHH
KESIEVLSDQSYKFNLPQRKITISTVGIIPGINELGKSGLKTKLAISLHSASQETRESLI
HHHHHHHCCCCEEECCCCCEEEEEEEECCCCHHHHCCCCCCEEEEEEEHHCCHHHHHHHC
PVASEFSLTQLRKTLSEYTSQTGEPVTLVYMLLKGINDSVEDARLLVKFSRSFLCKINLI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEEEEE
DYNSIINMKFKPVFNETKDMFIQHILDAGIHVTVRKSHGASINAACGQLAAKGTQKAENR
ECCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHCCCC
NNL
CCC
>Mature Secondary Structure
MQTRLSNIKHLSRQELRQAIANLGEPAYRTRQIHQWIFSHRAATFEEMTTISLELRNKLA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQFRIGFPILADCQQDGSANDPFSTVKLLLELDDNEKIETVLIPSENRMTACVSSQVGCP
HHHHCCCCEEECCCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCCC
LQCRFCASGQTGFKRNLSADEIIDQVFSLNDFIRTKHESNEITNIVFMGMGEPLLNFENL
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHCCHHHH
KESIEVLSDQSYKFNLPQRKITISTVGIIPGINELGKSGLKTKLAISLHSASQETRESLI
HHHHHHHCCCCEEECCCCCEEEEEEEECCCCHHHHCCCCCCEEEEEEEHHCCHHHHHHHC
PVASEFSLTQLRKTLSEYTSQTGEPVTLVYMLLKGINDSVEDARLLVKFSRSFLCKINLI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEEEEE
DYNSIINMKFKPVFNETKDMFIQHILDAGIHVTVRKSHGASINAACGQLAAKGTQKAENR
ECCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHCCCC
NNL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA