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The map label for this gene is thiD [H]

Identifier: 119357335

GI number: 119357335

Start: 1741050

End: 1741865

Strand: Direct

Name: thiD [H]

Synonym: Cpha266_1533

Alternate gene names: 119357335

Gene position: 1741050-1741865 (Clockwise)

Preceding gene: 119357334

Following gene: 119357341

Centisome position: 55.56

GC content: 47.92

Gene sequence:

>816_bases
ATGAAGAATAACTATACCACCATTCTCACCATCGCGGGTTCCGATGGCAGCGGAGGAGCGGGAATACAAGCTGATCTGAA
AACGTTTGCTGCGCTTGAATGCTATGGACTGTCCGTCATAACATCCGTCACGGCACAAAACACCGTGGAGGTAAAAGAAG
CCTTTGTTCTTTCAGGAAAACAGATTGAAGCTCAATTACTGGCACTCATCAGCGATATGTCCATTGATGCCATAAAAATA
GGAATGCCTGGAGAAATCGGGGGAATCAAAACCATCGCCAGAGTAATTGGCAACATGAAAACAAGACCGCCTGTCGTTCT
TGATACCATTATCAGCTCATCATCAGGCCAGGCGCTCCTTTCAGCCGAAGCCCTTGAGCCCTTTAAAAACGAGCTCTTTC
CTCTTGCTACACTTGTTACGCCGAACCTTATGGAAGCAATTGCGCTGACGGGTCGGAAACTGTCGATTGACTCACCGGAG
GCCGTAGAGGAAATCGCTCACACGCTTAACCTTATGGGGGCAGCATCGGTTCTTGTCAAAGGGGGCCATATGGAGGGATC
TCAATGCAATGACTGCCTTCTTCATAACAAGAAGATAAGATGGTATTCAGCAAAGAAAATCATTACGGGAAATACTCATG
GAACCGGCTGTACACTTTCATCGGCAATCGCTGCATATCTCGGTAAACACCTGCTGCTCGAAGAGGCTGTCGCGCAAGCA
AAATCTTACACTTATGATGCTTTAGAGGCAGGTGCCGCTTACCTTATCGGTAAGGGAAGCGGGCCGCTGCATCACTGTTA
TAAGCTCTGGAGATAA

Upstream 100 bases:

>100_bases
AGGAGCATCCGGAATCGCAATGATTGCCGCAATCAGCAGAACAGCCGACCCTGAAGGGGCAACCCGTGCAATTACAGAAC
TTCTGAAAGGTCATTGAAAC

Downstream 100 bases:

>100_bases
ACGTTCAATTGAGACCCCGCGGTCTGCGTCCTACAGAGTAATAGACAAACCCTGCCTGTTCCATATACTCAGGACTGTAA
ATATTTCTTCCGTCAAACAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKNNYTTILTIAGSDGSGGAGIQADLKTFAALECYGLSVITSVTAQNTVEVKEAFVLSGKQIEAQLLALISDMSIDAIKI
GMPGEIGGIKTIARVIGNMKTRPPVVLDTIISSSSGQALLSAEALEPFKNELFPLATLVTPNLMEAIALTGRKLSIDSPE
AVEEIAHTLNLMGAASVLVKGGHMEGSQCNDCLLHNKKIRWYSAKKIITGNTHGTGCTLSSAIAAYLGKHLLLEEAVAQA
KSYTYDALEAGAAYLIGKGSGPLHHCYKLWR

Sequences:

>Translated_271_residues
MKNNYTTILTIAGSDGSGGAGIQADLKTFAALECYGLSVITSVTAQNTVEVKEAFVLSGKQIEAQLLALISDMSIDAIKI
GMPGEIGGIKTIARVIGNMKTRPPVVLDTIISSSSGQALLSAEALEPFKNELFPLATLVTPNLMEAIALTGRKLSIDSPE
AVEEIAHTLNLMGAASVLVKGGHMEGSQCNDCLLHNKKIRWYSAKKIITGNTHGTGCTLSSAIAAYLGKHLLLEEAVAQA
KSYTYDALEAGAAYLIGKGSGPLHHCYKLWR
>Mature_271_residues
MKNNYTTILTIAGSDGSGGAGIQADLKTFAALECYGLSVITSVTAQNTVEVKEAFVLSGKQIEAQLLALISDMSIDAIKI
GMPGEIGGIKTIARVIGNMKTRPPVVLDTIISSSSGQALLSAEALEPFKNELFPLATLVTPNLMEAIALTGRKLSIDSPE
AVEEIAHTLNLMGAASVLVKGGHMEGSQCNDCLLHNKKIRWYSAKKIITGNTHGTGCTLSSAIAAYLGKHLLLEEAVAQA
KSYTYDALEAGAAYLIGKGSGPLHHCYKLWR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=267, Percent_Identity=42.3220973782771, Blast_Score=199, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=30.7971014492754, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324997, Length=275, Percent_Identity=32.7272727272727, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=30.8823529411765, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28588; Mature: 28588

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNYTTILTIAGSDGSGGAGIQADLKTFAALECYGLSVITSVTAQNTVEVKEAFVLSGK
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QIEAQLLALISDMSIDAIKIGMPGEIGGIKTIARVIGNMKTRPPVVLDTIISSSSGQALL
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEE
SAEALEPFKNELFPLATLVTPNLMEAIALTGRKLSIDSPEAVEEIAHTLNLMGAASVLVK
EHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHEE
GGHMEGSQCNDCLLHNKKIRWYSAKKIITGNTHGTGCTLSSAIAAYLGKHLLLEEAVAQA
CCCCCCCCCCHHHHCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KSYTYDALEAGAAYLIGKGSGPLHHCYKLWR
HHCCHHHHHCCCEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKNNYTTILTIAGSDGSGGAGIQADLKTFAALECYGLSVITSVTAQNTVEVKEAFVLSGK
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QIEAQLLALISDMSIDAIKIGMPGEIGGIKTIARVIGNMKTRPPVVLDTIISSSSGQALL
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEE
SAEALEPFKNELFPLATLVTPNLMEAIALTGRKLSIDSPEAVEEIAHTLNLMGAASVLVK
EHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHEE
GGHMEGSQCNDCLLHNKKIRWYSAKKIITGNTHGTGCTLSSAIAAYLGKHLLLEEAVAQA
CCCCCCCCCCHHHHCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KSYTYDALEAGAAYLIGKGSGPLHHCYKLWR
HHCCHHHHHCCCEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]