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The map label for this gene is thiE

Identifier: 119357334

GI number: 119357334

Start: 1740426

End: 1741046

Strand: Direct

Name: thiE

Synonym: Cpha266_1532

Alternate gene names: 119357334

Gene position: 1740426-1741046 (Clockwise)

Preceding gene: 119357333

Following gene: 119357335

Centisome position: 55.54

GC content: 49.11

Gene sequence:

>621_bases
ATGATACCTGCCAAACCATTTCTCTGTGTTATCACCGATGAGCAATGCTCATCACCCGTCGATCTGGCCTTGATGGCACT
CGAAGGCGGGGCTGAAATGATACAGCTTCGCCACAAATCAGCCTCAGGAAAACAACTTTTTCAATGGGCTCTGGATATTC
AGCGGCTTTGTCGGATCCATCATGCTCAATTTATTGTCAATGACCGGGTTGATATCGCTCTTGCCATGAATGCGGACGGA
GTACATCTGGGTCAGCAGGATCTTCAGCCCGGAGAGGCAAGAAAACTTCTTGGCACCGACAAGATAATAGGTGTTTCCAC
CTCATCTCTTACGGAGGCTTTAAATGCGGAGCGGGCTGGCGCCGACTACATTGGATTCGGCCATATTTTTCAAACCGGAT
CAAAAAACAAACTATCCGCGCCCCTTGGATCGGCGGCGATCAGTGCTGTCGTTCAACGGATTTCCATTCCTCTTGTTGCC
ATTGGAGGGATCAATAAGATGAATATGATGGAAACAATAGCAGCAGGAGCATCCGGAATCGCAATGATTGCCGCAATCAG
CAGAACAGCCGACCCTGAAGGGGCAACCCGTGCAATTACAGAACTTCTGAAAGGTCATTGA

Upstream 100 bases:

>100_bases
GCCTTGATTCCGGCGCATATGGAGTTGCCGCTCTCTCGCTGTTTATGGATACCACTCGCCTGGTCAATACGCTTGATAAA
TTTCATCGACTTCTGTATTC

Downstream 100 bases:

>100_bases
AACATGAAGAATAACTATACCACCATTCTCACCATCGCGGGTTCCGATGGCAGCGGAGGAGCGGGAATACAAGCTGATCT
GAAAACGTTTGCTGCGCTTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MIPAKPFLCVITDEQCSSPVDLALMALEGGAEMIQLRHKSASGKQLFQWALDIQRLCRIHHAQFIVNDRVDIALAMNADG
VHLGQQDLQPGEARKLLGTDKIIGVSTSSLTEALNAERAGADYIGFGHIFQTGSKNKLSAPLGSAAISAVVQRISIPLVA
IGGINKMNMMETIAAGASGIAMIAAISRTADPEGATRAITELLKGH

Sequences:

>Translated_206_residues
MIPAKPFLCVITDEQCSSPVDLALMALEGGAEMIQLRHKSASGKQLFQWALDIQRLCRIHHAQFIVNDRVDIALAMNADG
VHLGQQDLQPGEARKLLGTDKIIGVSTSSLTEALNAERAGADYIGFGHIFQTGSKNKLSAPLGSAAISAVVQRISIPLVA
IGGINKMNMMETIAAGASGIAMIAAISRTADPEGATRAITELLKGH
>Mature_206_residues
MIPAKPFLCVITDEQCSSPVDLALMALEGGAEMIQLRHKSASGKQLFQWALDIQRLCRIHHAQFIVNDRVDIALAMNADG
VHLGQQDLQPGEARKLLGTDKIIGVSTSSLTEALNAERAGADYIGFGHIFQTGSKNKLSAPLGSAAISAVVQRISIPLVA
IGGINKMNMMETIAAGASGIAMIAAISRTADPEGATRAITELLKGH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=177, Percent_Identity=34.4632768361582, Blast_Score=88, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=195, Percent_Identity=31.2820512820513, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CHLPD (A1BGM7)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911978.1
- ProteinModelPortal:   A1BGM7
- SMR:   A1BGM7
- STRING:   A1BGM7
- GeneID:   4569582
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1532
- NMPDR:   fig|290317.7.peg.1614
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   GARCCAL
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21738; Mature: 21738

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPAKPFLCVITDEQCSSPVDLALMALEGGAEMIQLRHKSASGKQLFQWALDIQRLCRIH
CCCCCCEEEEEECHHCCCCHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HAQFIVNDRVDIALAMNADGVHLGQQDLQPGEARKLLGTDKIIGVSTSSLTEALNAERAG
HHHHEECCCEEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHCC
ADYIGFGHIFQTGSKNKLSAPLGSAAISAVVQRISIPLVAIGGINKMNMMETIAAGASGI
CCCEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCHH
AMIAAISRTADPEGATRAITELLKGH
HHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIPAKPFLCVITDEQCSSPVDLALMALEGGAEMIQLRHKSASGKQLFQWALDIQRLCRIH
CCCCCCEEEEEECHHCCCCHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HAQFIVNDRVDIALAMNADGVHLGQQDLQPGEARKLLGTDKIIGVSTSSLTEALNAERAG
HHHHEECCCEEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHCC
ADYIGFGHIFQTGSKNKLSAPLGSAAISAVVQRISIPLVAIGGINKMNMMETIAAGASGI
CCCEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCHH
AMIAAISRTADPEGATRAITELLKGH
HHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA