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The map label for this gene is thiE [C]

Identifier: 119357333

GI number: 119357333

Start: 1739791

End: 1740429

Strand: Direct

Name: thiE [C]

Synonym: Cpha266_1531

Alternate gene names: 119357333

Gene position: 1739791-1740429 (Clockwise)

Preceding gene: 119357328

Following gene: 119357334

Centisome position: 55.52

GC content: 48.04

Gene sequence:

>639_bases
ATGACTCCCCCATCACCTCAACTTCCTCGCCTTTATCTGGTAAGCAGCGGCTTCAATTCCCGGGATTCTTATACTCAGCT
TGAAAATCAGGTTACCTGTTTTTCAAGTTCTTGCCCCTGCATTGTCCAAATACGAGAAAAGATGCTTGATGCCAAAACCC
TTTTTGAACTCTCCTGCTCCATTGCACCCGCCATCATTGCGGCAGGATCGATGGTTGTCATCAACGAGCGGGTTGATATT
GCCCTTGCTGCGGCACTCAATGGGGTACATTTTCCTGAAAACTCCTCTCCTCCAGAGAGATTCAGAGCGATGACAAAAGG
AAAAATTCTCGGTCAAAGCACTCATTCGCTTAAGACTGCTCGTATAAGCCAGCAGGCGGGGGTTGATTATATCCTGTTCG
GGCCAGTATTCGATACCCCCTCCAAAAGAGGTTTCGGGCCGCCTCAGGGTTTACTTAATCTCGCTGCTGTATGCGAGGCA
ACATCATTGCCGGTCTATGCAATCGGAGGAGTAACTCCTGAAAATGCTTCTTTATGCCTTGATTCCGGCGCATATGGAGT
TGCCGCTCTCTCGCTGTTTATGGATACCACTCGCCTGGTCAATACGCTTGATAAATTTCATCGACTTCTGTATTCATGA

Upstream 100 bases:

>100_bases
AACACCCATTTTACGCCGGAACGAGAAATTTCAGCTCCGTAAAAAAACGATCCGATTGCTGCACCCAGGCTAACAGAGAT
ACCCATAAACGATATCGCTC

Downstream 100 bases:

>100_bases
TACCTGCCAAACCATTTCTCTGTGTTATCACCGATGAGCAATGCTCATCACCCGTCGATCTGGCCTTGATGGCACTCGAA
GGCGGGGCTGAAATGATACA

Product: thiamine monophosphate synthase

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTPPSPQLPRLYLVSSGFNSRDSYTQLENQVTCFSSSCPCIVQIREKMLDAKTLFELSCSIAPAIIAAGSMVVINERVDI
ALAAALNGVHFPENSSPPERFRAMTKGKILGQSTHSLKTARISQQAGVDYILFGPVFDTPSKRGFGPPQGLLNLAAVCEA
TSLPVYAIGGVTPENASLCLDSGAYGVAALSLFMDTTRLVNTLDKFHRLLYS

Sequences:

>Translated_212_residues
MTPPSPQLPRLYLVSSGFNSRDSYTQLENQVTCFSSSCPCIVQIREKMLDAKTLFELSCSIAPAIIAAGSMVVINERVDI
ALAAALNGVHFPENSSPPERFRAMTKGKILGQSTHSLKTARISQQAGVDYILFGPVFDTPSKRGFGPPQGLLNLAAVCEA
TSLPVYAIGGVTPENASLCLDSGAYGVAALSLFMDTTRLVNTLDKFHRLLYS
>Mature_211_residues
TPPSPQLPRLYLVSSGFNSRDSYTQLENQVTCFSSSCPCIVQIREKMLDAKTLFELSCSIAPAIIAAGSMVVINERVDIA
LAAALNGVHFPENSSPPERFRAMTKGKILGQSTHSLKTARISQQAGVDYILFGPVFDTPSKRGFGPPQGLLNLAAVCEAT
SLPVYAIGGVTPENASLCLDSGAYGVAALSLFMDTTRLVNTLDKFHRLLYS

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 22761; Mature: 22630

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPSPQLPRLYLVSSGFNSRDSYTQLENQVTCFSSSCPCIVQIREKMLDAKTLFELSCS
CCCCCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
IAPAIIAAGSMVVINERVDIALAAALNGVHFPENSSPPERFRAMTKGKILGQSTHSLKTA
HHHHHHHCCCEEEEECCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
RISQQAGVDYILFGPVFDTPSKRGFGPPQGLLNLAAVCEATSLPVYAIGGVTPENASLCL
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEE
DSGAYGVAALSLFMDTTRLVNTLDKFHRLLYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPPSPQLPRLYLVSSGFNSRDSYTQLENQVTCFSSSCPCIVQIREKMLDAKTLFELSCS
CCCCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
IAPAIIAAGSMVVINERVDIALAAALNGVHFPENSSPPERFRAMTKGKILGQSTHSLKTA
HHHHHHHCCCEEEEECCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
RISQQAGVDYILFGPVFDTPSKRGFGPPQGLLNLAAVCEATSLPVYAIGGVTPENASLCL
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEE
DSGAYGVAALSLFMDTTRLVNTLDKFHRLLYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {hydroxymethylpyrimidine} 0.0004 {thiazole} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]