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The map label for this gene is mscL

Identifier: 119357075

GI number: 119357075

Start: 1435785

End: 1436231

Strand: Direct

Name: mscL

Synonym: Cpha266_1262

Alternate gene names: 119357075

Gene position: 1435785-1436231 (Clockwise)

Preceding gene: 119357068

Following gene: 119357076

Centisome position: 45.81

GC content: 47.2

Gene sequence:

>447_bases
ATGCTTAAAGAGTTTAAAGATTTTGCCGTTCGCGGCAATGTTGTTGATATGGCCGTAGGTATCATTATCGGGGCAGCATT
TACAACAATTATCAATACCCTTGTCAACGAAGTCGTGATGCCTCCCATAGGCGTTCTTCTTGGCGGGGTTGATTTTTCTG
ATTTTTATCTGCTTTTAAAAGAGGGGAGCAAGGCTGCGCCTTACGAATCGCTCGCTGCCGCAAAAAGCGCCGGAGCCGTT
ACGCTGAGTTACGGGATTTTTGTTAATGCCTGTATCAGTTTTTTAATAGTAACGTTCGTCATGTTCCTTTCGGTCAAGGG
CATTAACAGGCTGAGAGCAAAAGAGGACGCTGCTCCTGATCCTGCGGTAAGGGAGTGTCCTTTCTGCTGCAGTCCTGTGT
CCGTAAAAGCAAAACGCTGCCCGATGTGCACCTCTGAGCTCAAATGA

Upstream 100 bases:

>100_bases
TTTCCGGAAAGTTTCTGATTTCAGACAGCACACTTCGAAAATCGTCCCTATCTGCTCCTCCAAAACTTTATCCCGAAATT
TAAAAAAATAAACGCCGTTT

Downstream 100 bases:

>100_bases
GCCGGGCATGCGCTGCCCGATTATTGACGATGATCCTCCCTTTTTGTTTTAAAGCGGATAAAACGCAACCTGAAACGATC
CGCATTGACCTGAACATATG

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MLKEFKDFAVRGNVVDMAVGIIIGAAFTTIINTLVNEVVMPPIGVLLGGVDFSDFYLLLKEGSKAAPYESLAAAKSAGAV
TLSYGIFVNACISFLIVTFVMFLSVKGINRLRAKEDAAPDPAVRECPFCCSPVSVKAKRCPMCTSELK

Sequences:

>Translated_148_residues
MLKEFKDFAVRGNVVDMAVGIIIGAAFTTIINTLVNEVVMPPIGVLLGGVDFSDFYLLLKEGSKAAPYESLAAAKSAGAV
TLSYGIFVNACISFLIVTFVMFLSVKGINRLRAKEDAAPDPAVRECPFCCSPVSVKAKRCPMCTSELK
>Mature_148_residues
MLKEFKDFAVRGNVVDMAVGIIIGAAFTTIINTLVNEVVMPPIGVLLGGVDFSDFYLLLKEGSKAAPYESLAAAKSAGAV
TLSYGIFVNACISFLIVTFVMFLSVKGINRLRAKEDAAPDPAVRECPFCCSPVSVKAKRCPMCTSELK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=129, Percent_Identity=44.9612403100775, Blast_Score=116, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CHLPD (A1BFW8)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911719.1
- ProteinModelPortal:   A1BFW8
- SMR:   A1BFW8
- STRING:   A1BFW8
- GeneID:   4570396
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1262
- NMPDR:   fig|290317.7.peg.1334
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15803; Mature: 15803

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdcda240)-; HASH(0x10d36480)-;

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKDFAVRGNVVDMAVGIIIGAAFTTIINTLVNEVVMPPIGVLLGGVDFSDFYLLLK
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
EGSKAAPYESLAAAKSAGAVTLSYGIFVNACISFLIVTFVMFLSVKGINRLRAKEDAAPD
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
PAVRECPFCCSPVSVKAKRCPMCTSELK
CCHHCCCCCCCCCCCCHHCCCCCCCCCH
>Mature Secondary Structure
MLKEFKDFAVRGNVVDMAVGIIIGAAFTTIINTLVNEVVMPPIGVLLGGVDFSDFYLLLK
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
EGSKAAPYESLAAAKSAGAVTLSYGIFVNACISFLIVTFVMFLSVKGINRLRAKEDAAPD
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
PAVRECPFCCSPVSVKAKRCPMCTSELK
CCHHCCCCCCCCCCCCHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA