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The map label for this gene is nifL [H]

Identifier: 119357066

GI number: 119357066

Start: 1423437

End: 1425458

Strand: Direct

Name: nifL [H]

Synonym: Cpha266_1253

Alternate gene names: 119357066

Gene position: 1423437-1425458 (Clockwise)

Preceding gene: 119357065

Following gene: 119357068

Centisome position: 45.42

GC content: 44.91

Gene sequence:

>2022_bases
ATGAACCGAACTGGCGAGATCCCGACCATACTTGTTGCAGATAATTGTGTGATCAGTCGGAGGTCAATATCCGATGCAAT
CTGTGAGCAGGGCTATAAAGCAGTAGTCGCAGAGGATGGAGCTGGCTGTAGGGATCTTCTTGGTAAGGATAAAACAGATC
TTCTTTTTCTGGATGCCGGTATTGATGATGACAAGGGCGTCGAATTACTTGTCGCTGTTAAAGATAATTATCCGGATATT
CCGGTCATTGTAATTTCCTCATCAATCTCGTTTGAGCAGGAAGCTCTCTTTTTTAAGATGGGAGCGAGTGAATATCTGAT
AAAACCGCTCAGTTCCGTCAGGCTTGCCGTCACCATCAAAAAAGCACTTTTGGAATCGGAATACCGAAAAAAGGATAAAC
TGTTTTCGGTGGTGATTACCCACAGCCCCATAGCAGTGGCTATAACCGACAATACCGGACATTTTGAGTATGTCAATCAT
GCTTTTTGCAAAACAACCGGTTATGCATTCCATGAAGTAATCGGCAAAACACCCCGGTTGTTACAGTCCGGAGAACATGC
CGAAAGTTTCTATAAAGAACTATGGGATACTATCCGCTCCGGAAAAAACTGGCAGGGCGAATTTCACAACAAAAAAAAAA
CCGGAGAACTCTACTGGGAAAACTCGATCATCATCCCGATTTTCGATCCATCAGGAACGCTTTCCCACTTTTTAAGCATC
AAGCAGGACATCTCTCTTCGTAAAAAAGAACAGGAAGCCCTTCTGCAAAGTGAACGTCGCTTTCAGGAACTTGCCGATCT
TCTTCCCCTCCCTCTTATTGAAACCGATACGGAGGGTATGATTACCTATACTAATTGTGTAGGCCTTGAAGCATTCGGGT
ATACAACAGAGGATCTTGCAAACGGTATTCATGGCGTCATGCTCTTTTTACCTGAAGAAAGGCAGAGAGTTATACTCAAC
ATAGAAATGCGGTTGAAAAATATTCCGTTTGAGAATCATGAGTATATCGGCCTGAGAAAAGACGGAACCACCTTTCCTCT
TCTTGTTTATACCGCAAGCATAATGCATAACAACGTTCCTGTGGGCATTCGTGCTATTGTGCTTGATATTACAAATCGTA
AGCAAGCCGAAGAAAAGCTTATGCAGCTCAATCAGACTCTTGAACTCAGGGTGCAGGAGCGAACCAGAGAGCTTGAAATT
ACGCATAAGCAGATGATTCTGCAGGAAAAACTTGCTTCCATTGGTCAGTTGGCCGCCGGTATCGCCCATGAACTGAACAA
TCCCATCAACTTTGTCAGAATCAATTTCGCAACACTGGAGGAAGACATTGCTGATCTGCAGAGCCTTCTCAATGCGTATC
GCAACGTCATTGACAAAGTCGGTAAAGATCTTTTACCAAAAGATGAGGTGGATCGGCTGCGTCAGCTTGAGCACTCTCTT
GCTATAGACACGCTGTGTGATGAGCTACCCGGAATTTTTAGTGAATCACATCGGGGTTTTGAGCGAATCACAGCTATTAT
CGGAAGCATGCGGAACTTTTCGTTCTGTCACGACTTACAGGAACGGGTACCGTTTTATATCAATACAGGGATTCGGGATG
CGCTTATCATTACCAGAAACGAATATCTATCCTTTGCTGATTTGACCACGCAACTTGAAGATCTGCCGCCGGTTCCCTGT
AATCCCGAGCAGATCAACCAGGTTTTTCTCAATCTTATTATTAACAGCGCTCAGGCCATTGAATCGCAAAAGAGGTCCGA
AAACGGAAAAATCACCATTTGCACCTGGTTCGACAACCGGAGTGTTTTCTGTGCTGTAACCGATGACGGGCCGGGTATTC
CTTGTGAAATCCAGCACCGAATTTTCGAACCATTTTTCACCACGAAGCCACCGGGAAAAGGTACCGGCCTCGGTCTGAGC
ATCTCCTACGATATCGTTGTTCATAAACATGGAGGAACTCTTGATGTTCATTGTCCGCCTGAAGGGGGAACCGTTATTAC
CCTGTCGCTTCCTCTTGGTTGA

Upstream 100 bases:

>100_bases
TATCGCTCATGACTTGTATCACCCTCAAAAAGCGTTTGTGAGCCGTAGTGATCAGCAGTGAATGATGATCTGAGAACAAT
TTTATGGCAATCAGTATACA

Downstream 100 bases:

>100_bases
GCAGGCGAAACCTGCGCTTTCATGAAAAATCGTGCGGCTTTGCTTGCGGATCGCAAAAAGATGTTTCGTGATGTACAGCA
AGCCGATCATTGCCAGAGAT

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MNRTGEIPTILVADNCVISRRSISDAICEQGYKAVVAEDGAGCRDLLGKDKTDLLFLDAGIDDDKGVELLVAVKDNYPDI
PVIVISSSISFEQEALFFKMGASEYLIKPLSSVRLAVTIKKALLESEYRKKDKLFSVVITHSPIAVAITDNTGHFEYVNH
AFCKTTGYAFHEVIGKTPRLLQSGEHAESFYKELWDTIRSGKNWQGEFHNKKKTGELYWENSIIIPIFDPSGTLSHFLSI
KQDISLRKKEQEALLQSERRFQELADLLPLPLIETDTEGMITYTNCVGLEAFGYTTEDLANGIHGVMLFLPEERQRVILN
IEMRLKNIPFENHEYIGLRKDGTTFPLLVYTASIMHNNVPVGIRAIVLDITNRKQAEEKLMQLNQTLELRVQERTRELEI
THKQMILQEKLASIGQLAAGIAHELNNPINFVRINFATLEEDIADLQSLLNAYRNVIDKVGKDLLPKDEVDRLRQLEHSL
AIDTLCDELPGIFSESHRGFERITAIIGSMRNFSFCHDLQERVPFYINTGIRDALIITRNEYLSFADLTTQLEDLPPVPC
NPEQINQVFLNLIINSAQAIESQKRSENGKITICTWFDNRSVFCAVTDDGPGIPCEIQHRIFEPFFTTKPPGKGTGLGLS
ISYDIVVHKHGGTLDVHCPPEGGTVITLSLPLG

Sequences:

>Translated_673_residues
MNRTGEIPTILVADNCVISRRSISDAICEQGYKAVVAEDGAGCRDLLGKDKTDLLFLDAGIDDDKGVELLVAVKDNYPDI
PVIVISSSISFEQEALFFKMGASEYLIKPLSSVRLAVTIKKALLESEYRKKDKLFSVVITHSPIAVAITDNTGHFEYVNH
AFCKTTGYAFHEVIGKTPRLLQSGEHAESFYKELWDTIRSGKNWQGEFHNKKKTGELYWENSIIIPIFDPSGTLSHFLSI
KQDISLRKKEQEALLQSERRFQELADLLPLPLIETDTEGMITYTNCVGLEAFGYTTEDLANGIHGVMLFLPEERQRVILN
IEMRLKNIPFENHEYIGLRKDGTTFPLLVYTASIMHNNVPVGIRAIVLDITNRKQAEEKLMQLNQTLELRVQERTRELEI
THKQMILQEKLASIGQLAAGIAHELNNPINFVRINFATLEEDIADLQSLLNAYRNVIDKVGKDLLPKDEVDRLRQLEHSL
AIDTLCDELPGIFSESHRGFERITAIIGSMRNFSFCHDLQERVPFYINTGIRDALIITRNEYLSFADLTTQLEDLPPVPC
NPEQINQVFLNLIINSAQAIESQKRSENGKITICTWFDNRSVFCAVTDDGPGIPCEIQHRIFEPFFTTKPPGKGTGLGLS
ISYDIVVHKHGGTLDVHCPPEGGTVITLSLPLG
>Mature_673_residues
MNRTGEIPTILVADNCVISRRSISDAICEQGYKAVVAEDGAGCRDLLGKDKTDLLFLDAGIDDDKGVELLVAVKDNYPDI
PVIVISSSISFEQEALFFKMGASEYLIKPLSSVRLAVTIKKALLESEYRKKDKLFSVVITHSPIAVAITDNTGHFEYVNH
AFCKTTGYAFHEVIGKTPRLLQSGEHAESFYKELWDTIRSGKNWQGEFHNKKKTGELYWENSIIIPIFDPSGTLSHFLSI
KQDISLRKKEQEALLQSERRFQELADLLPLPLIETDTEGMITYTNCVGLEAFGYTTEDLANGIHGVMLFLPEERQRVILN
IEMRLKNIPFENHEYIGLRKDGTTFPLLVYTASIMHNNVPVGIRAIVLDITNRKQAEEKLMQLNQTLELRVQERTRELEI
THKQMILQEKLASIGQLAAGIAHELNNPINFVRINFATLEEDIADLQSLLNAYRNVIDKVGKDLLPKDEVDRLRQLEHSL
AIDTLCDELPGIFSESHRGFERITAIIGSMRNFSFCHDLQERVPFYINTGIRDALIITRNEYLSFADLTTQLEDLPPVPC
NPEQINQVFLNLIINSAQAIESQKRSENGKITICTWFDNRSVFCAVTDDGPGIPCEIQHRIFEPFFTTKPPGKGTGLGLS
ISYDIVVHKHGGTLDVHCPPEGGTVITLSLPLG

Specific function: Required for the inhibition of nifA activity in response to oxygen and low level of fixed nitrogen [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=273, Percent_Identity=29.3040293040293, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790436, Length=277, Percent_Identity=28.8808664259928, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790300, Length=288, Percent_Identity=28.4722222222222, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR014285
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75655; Mature: 75655

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTGEIPTILVADNCVISRRSISDAICEQGYKAVVAEDGAGCRDLLGKDKTDLLFLDAG
CCCCCCCCEEEEECCEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCEEEEECC
IDDDKGVELLVAVKDNYPDIPVIVISSSISFEQEALFFKMGASEYLIKPLSSVRLAVTIK
CCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHEEEECCCHHHHHHCHHHHEEHHHHH
KALLESEYRKKDKLFSVVITHSPIAVAITDNTGHFEYVNHAFCKTTGYAFHEVIGKTPRL
HHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHH
LQSGEHAESFYKELWDTIRSGKNWQGEFHNKKKTGELYWENSIIIPIFDPSGTLSHFLSI
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHH
KQDISLRKKEQEALLQSERRFQELADLLPLPLIETDTEGMITYTNCVGLEAFGYTTEDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEECEEECCCCHHHHH
NGIHGVMLFLPEERQRVILNIEMRLKNIPFENHEYIGLRKDGTTFPLLVYTASIMHNNVP
CCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
VGIRAIVLDITNRKQAEEKLMQLNQTLELRVQERTRELEITHKQMILQEKLASIGQLAAG
CEEEEEEEEECCCHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHELNNPINFVRINFATLEEDIADLQSLLNAYRNVIDKVGKDLLPKDEVDRLRQLEHSL
HHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIDTLCDELPGIFSESHRGFERITAIIGSMRNFSFCHDLQERVPFYINTGIRDALIITRN
HHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEEC
EYLSFADLTTQLEDLPPVPCNPEQINQVFLNLIINSAQAIESQKRSENGKITICTWFDNR
CCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SVFCAVTDDGPGIPCEIQHRIFEPFFTTKPPGKGTGLGLSISYDIVVHKHGGTLDVHCPP
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCEEEEEECC
EGGTVITLSLPLG
CCCEEEEEEECCC
>Mature Secondary Structure
MNRTGEIPTILVADNCVISRRSISDAICEQGYKAVVAEDGAGCRDLLGKDKTDLLFLDAG
CCCCCCCCEEEEECCEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCEEEEECC
IDDDKGVELLVAVKDNYPDIPVIVISSSISFEQEALFFKMGASEYLIKPLSSVRLAVTIK
CCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHEEEECCCHHHHHHCHHHHEEHHHHH
KALLESEYRKKDKLFSVVITHSPIAVAITDNTGHFEYVNHAFCKTTGYAFHEVIGKTPRL
HHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHH
LQSGEHAESFYKELWDTIRSGKNWQGEFHNKKKTGELYWENSIIIPIFDPSGTLSHFLSI
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHH
KQDISLRKKEQEALLQSERRFQELADLLPLPLIETDTEGMITYTNCVGLEAFGYTTEDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEECEEECCCCHHHHH
NGIHGVMLFLPEERQRVILNIEMRLKNIPFENHEYIGLRKDGTTFPLLVYTASIMHNNVP
CCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
VGIRAIVLDITNRKQAEEKLMQLNQTLELRVQERTRELEITHKQMILQEKLASIGQLAAG
CEEEEEEEEECCCHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHELNNPINFVRINFATLEEDIADLQSLLNAYRNVIDKVGKDLLPKDEVDRLRQLEHSL
HHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIDTLCDELPGIFSESHRGFERITAIIGSMRNFSFCHDLQERVPFYINTGIRDALIITRN
HHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEEC
EYLSFADLTTQLEDLPPVPCNPEQINQVFLNLIINSAQAIESQKRSENGKITICTWFDNR
CCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SVFCAVTDDGPGIPCEIQHRIFEPFFTTKPPGKGTGLGLSISYDIVVHKHGGTLDVHCPP
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCEEEEEECC
EGGTVITLSLPLG
CCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8231815; 2840552; 9601070; 9593306 [H]