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The map label for this gene is btuB [H]

Identifier: 119356935

GI number: 119356935

Start: 1260809

End: 1262758

Strand: Direct

Name: btuB [H]

Synonym: Cpha266_1115

Alternate gene names: 119356935

Gene position: 1260809-1262758 (Clockwise)

Preceding gene: 119356934

Following gene: 119356936

Centisome position: 40.23

GC content: 47.79

Gene sequence:

>1950_bases
ATGAACAAAAAAATTTTTCTTATCGTGATGGCCGGGCTGCTGTGCAGCAAAGGACTTCTTGCCGAAGAGATGGTAAAAAG
CTATACCGGGGATGAGGTTGTGGTGACTTCAAGCCGTGTAGAGGAGTCTAAAAAAAATGTAACCTCCAGTATTACCGTTA
TTGGCAGGGAAGAGATCGAACAATCATCTGCTCACGATCTCGGTGAATTGCTTGCGGAAAAAAGCATTGGGCATATCCAG
AAATATCCAGGCACCATGACATCGGTTGGTATCAGGGGATTCAGGACCGAGACGCATGGTAACGATCTTATGGGCAAGGT
GCTTGTGCTTCTCAATGGCCGGCGCGCGGGTACCGGCAACCTTGCCAAAATTTCGACGGGGAATATAGAACGTATTGAGA
TTATTCGTGGCCCGGCTGCAGTGCAATACGGTTCTGCTGCCATAGGAGGTATCGTTAACGTTATCACCTCAAGGGGAAAC
GGAAAACCGGGATTGTTTTTTAAACAGGAATTCGGGAGCAATGATTACACCCGGACGACCATGGGGGCCGATGGCAAATC
AGCCGGGGTTGATTATTCGGGCAGTGTTTCTTTTTCTGATGCGGGTGATTATAAGACCGGTGCAGGAGATGAATACAAAA
ATACCGGTTACAGTGACAGGACGGTCGGAAATGTGAATATCGGTTACGAGTTTCTGCCCCATCACAGAATAGGCGTGATA
TACAATTATTTCGATGTCAGAAAAGCAGGTTCCCCATCATATCTGAGTCAGAATGATTTGGATGCATATACCAGTCAGAA
AGCATATTCTACCGATTTTATCTATGAGGGCTCTACCGGGAACCGGCGTTTTTCATGGATGGCTCGTTATTTTACCGGCC
GTGACGAATACCTCTATGCGGATCCCGGCACATCGTATTCAAGTACAAATGATGTCGAACATAAAGGTGCGCAAGCCCAG
CTCACCTATGACGATGAGTCGTTGCGGGTTACCGCCGGTGTGGACTGGATCAGATATGATCTTGAGTCTACACTCGCCCC
CTATTCTTCAGAATATGAAAACCCGGCATATTTTCTTCTTGGCAAGTATGGCTTCATGTCAGACAGACTCGTGCTTACAG
CAGGTCTTCGTTATGATCAATATGACGTAACCATTTCAGAGGGGCAGGGTTCTTCAAGAAGAACCGATAACCTTGCCGAA
CAGGTTGGGCTGGCCTGGAATGTATCAGACGCGCTGAAGCTGAGAGCCTCGTATGCCGAAGGGTTCAGGATGCCGTCGGC
AAGAGAGCTGGTCGCCGATTTTTACTCATGGGGTACTCATTATATCGGTAACCCGGATCTTAAACCGGAAAAGAGCCGGT
CGTACGAAGCCGGAGTTGATATTGATTACCGGAATGTAAAATCATCGCTGACCTGGTTTACTACGGATTTCCGGGACAAG
ATTCAATCCACGGCCGTCGATGGCGCTATGAGCTGGATCAATATCGGAGGAGCGACGGTTTCCGGACTTGAAGCGGAATT
TTCATTGGCATTGCACCCTTTCGGAGAGGAGTGGAAAATTGAGCCGTATGTAAACTGTACCTGGTTGACGAAGTATCGTG
ATGAGGATACAGGCGAACTCCTGACCTATACGCCGGACCTGTCTGCTACAACGGGAATCAGGGTATCCGACCAGCGAGGT
TTCAGCAGTGTTTTTAATCTCGCTTATACCGGAGAAACACGAGTTCAGAACTGGGAGGACTGGTCAGGTGATATTGTTGT
CAAGGGGGGATATGCCGTAGCTAACATATCGGTTTCGAAAAAGTTTCTTGTTGATGAGGATAAAAAGAACGGCAGAGGGA
TAACCATCAAGGGCGAGATCGATAATCTCTTCGATCGCGAATTTCAGTACGTAAAAGGCTATACCATGCCTGGACGTACA
TTTACTATCGGCCTTGGAGCTGATATTTAG

Upstream 100 bases:

>100_bases
CCCAAAGTCCCGACTTGTCGGGACAGCGCAGCAATCTGGTTTCGGAAGAAATAAATAGAGGTAACCTGATGTGTTTCTTA
AACTGAAAACAGGTGAATCA

Downstream 100 bases:

>100_bases
TTCCTCCGGGATCGTGAAGCATAAAACAGAAAAAAAGAACATATGAATACCGCTATAACCGAAATCGAAAGGAAAGCTCT
ATACAAGGTGATCTACAGCC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MNKKIFLIVMAGLLCSKGLLAEEMVKSYTGDEVVVTSSRVEESKKNVTSSITVIGREEIEQSSAHDLGELLAEKSIGHIQ
KYPGTMTSVGIRGFRTETHGNDLMGKVLVLLNGRRAGTGNLAKISTGNIERIEIIRGPAAVQYGSAAIGGIVNVITSRGN
GKPGLFFKQEFGSNDYTRTTMGADGKSAGVDYSGSVSFSDAGDYKTGAGDEYKNTGYSDRTVGNVNIGYEFLPHHRIGVI
YNYFDVRKAGSPSYLSQNDLDAYTSQKAYSTDFIYEGSTGNRRFSWMARYFTGRDEYLYADPGTSYSSTNDVEHKGAQAQ
LTYDDESLRVTAGVDWIRYDLESTLAPYSSEYENPAYFLLGKYGFMSDRLVLTAGLRYDQYDVTISEGQGSSRRTDNLAE
QVGLAWNVSDALKLRASYAEGFRMPSARELVADFYSWGTHYIGNPDLKPEKSRSYEAGVDIDYRNVKSSLTWFTTDFRDK
IQSTAVDGAMSWINIGGATVSGLEAEFSLALHPFGEEWKIEPYVNCTWLTKYRDEDTGELLTYTPDLSATTGIRVSDQRG
FSSVFNLAYTGETRVQNWEDWSGDIVVKGGYAVANISVSKKFLVDEDKKNGRGITIKGEIDNLFDREFQYVKGYTMPGRT
FTIGLGADI

Sequences:

>Translated_649_residues
MNKKIFLIVMAGLLCSKGLLAEEMVKSYTGDEVVVTSSRVEESKKNVTSSITVIGREEIEQSSAHDLGELLAEKSIGHIQ
KYPGTMTSVGIRGFRTETHGNDLMGKVLVLLNGRRAGTGNLAKISTGNIERIEIIRGPAAVQYGSAAIGGIVNVITSRGN
GKPGLFFKQEFGSNDYTRTTMGADGKSAGVDYSGSVSFSDAGDYKTGAGDEYKNTGYSDRTVGNVNIGYEFLPHHRIGVI
YNYFDVRKAGSPSYLSQNDLDAYTSQKAYSTDFIYEGSTGNRRFSWMARYFTGRDEYLYADPGTSYSSTNDVEHKGAQAQ
LTYDDESLRVTAGVDWIRYDLESTLAPYSSEYENPAYFLLGKYGFMSDRLVLTAGLRYDQYDVTISEGQGSSRRTDNLAE
QVGLAWNVSDALKLRASYAEGFRMPSARELVADFYSWGTHYIGNPDLKPEKSRSYEAGVDIDYRNVKSSLTWFTTDFRDK
IQSTAVDGAMSWINIGGATVSGLEAEFSLALHPFGEEWKIEPYVNCTWLTKYRDEDTGELLTYTPDLSATTGIRVSDQRG
FSSVFNLAYTGETRVQNWEDWSGDIVVKGGYAVANISVSKKFLVDEDKKNGRGITIKGEIDNLFDREFQYVKGYTMPGRT
FTIGLGADI
>Mature_649_residues
MNKKIFLIVMAGLLCSKGLLAEEMVKSYTGDEVVVTSSRVEESKKNVTSSITVIGREEIEQSSAHDLGELLAEKSIGHIQ
KYPGTMTSVGIRGFRTETHGNDLMGKVLVLLNGRRAGTGNLAKISTGNIERIEIIRGPAAVQYGSAAIGGIVNVITSRGN
GKPGLFFKQEFGSNDYTRTTMGADGKSAGVDYSGSVSFSDAGDYKTGAGDEYKNTGYSDRTVGNVNIGYEFLPHHRIGVI
YNYFDVRKAGSPSYLSQNDLDAYTSQKAYSTDFIYEGSTGNRRFSWMARYFTGRDEYLYADPGTSYSSTNDVEHKGAQAQ
LTYDDESLRVTAGVDWIRYDLESTLAPYSSEYENPAYFLLGKYGFMSDRLVLTAGLRYDQYDVTISEGQGSSRRTDNLAE
QVGLAWNVSDALKLRASYAEGFRMPSARELVADFYSWGTHYIGNPDLKPEKSRSYEAGVDIDYRNVKSSLTWFTTDFRDK
IQSTAVDGAMSWINIGGATVSGLEAEFSLALHPFGEEWKIEPYVNCTWLTKYRDEDTGELLTYTPDLSATTGIRVSDQRG
FSSVFNLAYTGETRVQNWEDWSGDIVVKGGYAVANISVSKKFLVDEDKKNGRGITIKGEIDNLFDREFQYVKGYTMPGRT
FTIGLGADI

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=586, Percent_Identity=25.2559726962457, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790405, Length=695, Percent_Identity=24.1726618705036, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1786798, Length=315, Percent_Identity=20.952380952381, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 71737; Mature: 71737

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIFLIVMAGLLCSKGLLAEEMVKSYTGDEVVVTSSRVEESKKNVTSSITVIGREEIE
CCCEEEEEEEHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEEHHHHH
QSSAHDLGELLAEKSIGHIQKYPGTMTSVGIRGFRTETHGNDLMGKVLVLLNGRRAGTGN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCHHHEEEEEEEECCCCCCCC
LAKISTGNIERIEIIRGPAAVQYGSAAIGGIVNVITSRGNGKPGLFFKQEFGSNDYTRTT
EEEEECCCCEEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEE
MGADGKSAGVDYSGSVSFSDAGDYKTGAGDEYKNTGYSDRTVGNVNIGYEFLPHHRIGVI
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEE
YNYFDVRKAGSPSYLSQNDLDAYTSQKAYSTDFIYEGSTGNRRFSWMARYFTGRDEYLYA
EEEEEHCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCHHEEHHHHHCCCCCEEEE
DPGTSYSSTNDVEHKGAQAQLTYDDESLRVTAGVDWIRYDLESTLAPYSSEYENPAYFLL
CCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCEEEEEHHHHCCCCCCCCCCCEEEEE
GKYGFMSDRLVLTAGLRYDQYDVTISEGQGSSRRTDNLAEQVGLAWNVSDALKLRASYAE
ECCCCCCCCEEEEECCEECEEEEEEECCCCCCCCHHHHHHHHCCEECCHHHHHHHHHHHH
GFRMPSARELVADFYSWGTHYIGNPDLKPEKSRSYEAGVDIDYRNVKSSLTWFTTDFRDK
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCCCEEEEECHHHHH
IQSTAVDGAMSWINIGGATVSGLEAEFSLALHPFGEEWKIEPYVNCTWLTKYRDEDTGEL
HHHHHHHHHHHEEECCCCEECCCCEEEEEEECCCCCCEEECCEEEEEEEEEECCCCCCCE
LTYTPDLSATTGIRVSDQRGFSSVFNLAYTGETRVQNWEDWSGDIVVKGGYAVANISVSK
EEECCCCCCCCCEEEECCCCCHHHHEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEECE
KFLVDEDKKNGRGITIKGEIDNLFDREFQYVKGYTMPGRTFTIGLGADI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MNKKIFLIVMAGLLCSKGLLAEEMVKSYTGDEVVVTSSRVEESKKNVTSSITVIGREEIE
CCCEEEEEEEHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEEHHHHH
QSSAHDLGELLAEKSIGHIQKYPGTMTSVGIRGFRTETHGNDLMGKVLVLLNGRRAGTGN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCHHHEEEEEEEECCCCCCCC
LAKISTGNIERIEIIRGPAAVQYGSAAIGGIVNVITSRGNGKPGLFFKQEFGSNDYTRTT
EEEEECCCCEEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEE
MGADGKSAGVDYSGSVSFSDAGDYKTGAGDEYKNTGYSDRTVGNVNIGYEFLPHHRIGVI
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEE
YNYFDVRKAGSPSYLSQNDLDAYTSQKAYSTDFIYEGSTGNRRFSWMARYFTGRDEYLYA
EEEEEHCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCHHEEHHHHHCCCCCEEEE
DPGTSYSSTNDVEHKGAQAQLTYDDESLRVTAGVDWIRYDLESTLAPYSSEYENPAYFLL
CCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCEEEEEHHHHCCCCCCCCCCCEEEEE
GKYGFMSDRLVLTAGLRYDQYDVTISEGQGSSRRTDNLAEQVGLAWNVSDALKLRASYAE
ECCCCCCCCEEEEECCEECEEEEEEECCCCCCCCHHHHHHHHCCEECCHHHHHHHHHHHH
GFRMPSARELVADFYSWGTHYIGNPDLKPEKSRSYEAGVDIDYRNVKSSLTWFTTDFRDK
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCCCEEEEECHHHHH
IQSTAVDGAMSWINIGGATVSGLEAEFSLALHPFGEEWKIEPYVNCTWLTKYRDEDTGEL
HHHHHHHHHHHEEECCCCEECCCCEEEEEEECCCCCCEEECCEEEEEEEEEECCCCCCCE
LTYTPDLSATTGIRVSDQRGFSSVFNLAYTGETRVQNWEDWSGDIVVKGGYAVANISVSK
EEECCCCCCCCCEEEECCCCCHHHHEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEECE
KFLVDEDKKNGRGITIKGEIDNLFDREFQYVKGYTMPGRTFTIGLGADI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]