Click here to switch to the map view.

The map label for this gene is adh1 [H]

Identifier: 119356831

GI number: 119356831

Start: 1141515

End: 1142666

Strand: Direct

Name: adh1 [H]

Synonym: Cpha266_1004

Alternate gene names: 119356831

Gene position: 1141515-1142666 (Clockwise)

Preceding gene: 119356830

Following gene: 119356832

Centisome position: 36.42

GC content: 50.78

Gene sequence:

>1152_bases
ATGCCGGCAATTCATTTTGGCGCCGGTATGTTTGATCGCCTTGTCGATTTTTCCCTTCCTTATGGTCGAAACGCTCTTCT
TGTTACCGGCAGAGGTTCGCTGCAACGGCAGGGTTATCTCCACTCTCTTTTTAATTCGTTCAGCAAGGCAGGTATACAGT
ATGCCCATATCATTGTTGACCATGAGCCATCTCCCGAGCTGATAGATCATGCAGTGTCCTGTTACAGGGATCTTTCGATT
GATGTTGTTCTTGCCGTTGGCGGCGGAAGCGTGATCGATGCAGGCAAGGCTATTGCAGCAATGCTTCCCTCTGGTGATGC
GGTTGAGCGATATATTGAAGGTTTACCGGGCGCTGTTCCGCACAATGGAAGAAAAGTGCCGTTTATTGCTGTTCCGACCA
CCTCCGGCACTGGCAGTGAGGTGACCAATAATGCTGTTATCAGCCGAACCGGAAAAAACGGGTTCAAGCGTTCGCTTCGT
CACTCATCCTTTGTACCGGATGTTGCCGTTGTTGATCCTTTGTTGATGTGTTCTGCTTCCCGGGAACTTACCGCCTCGTC
AGGTATGGATGCCTGCACCCAGCTTCTTGAAGCGTACGTGTCGCCTTTTGCGACGCCGTATACCGATCTTCTTGCCTTTC
AGGGGCTTGAATATTTTGCACGATCATTTCTCTCGGCCTGTTCTGACGGTGCCGATGATTCTGCGGTACGTGCCGATATG
GCATATGCTGCGCTTCTTTCGGGTGTTGTCCTTTCTAATGCCGGACTTGGTATTGTGCACGGGTTTGCCTCTTCTGTTGG
CGGGGAGTACGATATTCCCCATGGAATGCTATGCGCCACCCTGCTTGCCGAAGCGACACGGACAAACATTCGCGAGTTGC
GTGCCAGCCGAAACGAATATCCTGCTTTGAAAAAATATGCGCAGGCAGGACAACTGTTTTCAGGCAGAGATGCAACGGAT
TGTTCTGAAGGGTGCAATATGCTGATTGAAAAGCTTGAGGAGTGGCAGCAGCAGCTTGCAATACCAAAGCTCAGCGCTTA
TGGAATCGGTATCTTCGACACAGAGCGCCTTGCCTCTCGAACTCGCAGCAAGAGCAACCCTGTTGATCTCACTCTTCAAA
GTATGCAAGCTATTCTCGCTGCGAGAGTTTAG

Upstream 100 bases:

>100_bases
TGCAGAACACATATCTGCCCTGAGTGCAGCTCTCCGCTTATCCGTAAAGGGAGGTGCAGCAGTGTTGTCTGATGCGCTTC
CTCTTTTTTCACTCCTTTCC

Downstream 100 bases:

>100_bases
GCTGATATATCGAGATCAGAGTCCGGAAGAGATGCCTTTTTTCGTTTTGCGATGACAATCGGGTTGTTCGGGATGATCGA
ATGTCAACGTTCAGAGCCCT

Product: iron-containing alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPAIHFGAGMFDRLVDFSLPYGRNALLVTGRGSLQRQGYLHSLFNSFSKAGIQYAHIIVDHEPSPELIDHAVSCYRDLSI
DVVLAVGGGSVIDAGKAIAAMLPSGDAVERYIEGLPGAVPHNGRKVPFIAVPTTSGTGSEVTNNAVISRTGKNGFKRSLR
HSSFVPDVAVVDPLLMCSASRELTASSGMDACTQLLEAYVSPFATPYTDLLAFQGLEYFARSFLSACSDGADDSAVRADM
AYAALLSGVVLSNAGLGIVHGFASSVGGEYDIPHGMLCATLLAEATRTNIRELRASRNEYPALKKYAQAGQLFSGRDATD
CSEGCNMLIEKLEEWQQQLAIPKLSAYGIGIFDTERLASRTRSKSNPVDLTLQSMQAILAARV

Sequences:

>Translated_383_residues
MPAIHFGAGMFDRLVDFSLPYGRNALLVTGRGSLQRQGYLHSLFNSFSKAGIQYAHIIVDHEPSPELIDHAVSCYRDLSI
DVVLAVGGGSVIDAGKAIAAMLPSGDAVERYIEGLPGAVPHNGRKVPFIAVPTTSGTGSEVTNNAVISRTGKNGFKRSLR
HSSFVPDVAVVDPLLMCSASRELTASSGMDACTQLLEAYVSPFATPYTDLLAFQGLEYFARSFLSACSDGADDSAVRADM
AYAALLSGVVLSNAGLGIVHGFASSVGGEYDIPHGMLCATLLAEATRTNIRELRASRNEYPALKKYAQAGQLFSGRDATD
CSEGCNMLIEKLEEWQQQLAIPKLSAYGIGIFDTERLASRTRSKSNPVDLTLQSMQAILAARV
>Mature_382_residues
PAIHFGAGMFDRLVDFSLPYGRNALLVTGRGSLQRQGYLHSLFNSFSKAGIQYAHIIVDHEPSPELIDHAVSCYRDLSID
VVLAVGGGSVIDAGKAIAAMLPSGDAVERYIEGLPGAVPHNGRKVPFIAVPTTSGTGSEVTNNAVISRTGKNGFKRSLRH
SSFVPDVAVVDPLLMCSASRELTASSGMDACTQLLEAYVSPFATPYTDLLAFQGLEYFARSFLSACSDGADDSAVRADMA
YAALLSGVVLSNAGLGIVHGFASSVGGEYDIPHGMLCATLLAEATRTNIRELRASRNEYPALKKYAQAGQLFSGRDATDC
SEGCNMLIEKLEEWQQQLAIPKLSAYGIGIFDTERLASRTRSKSNPVDLTLQSMQAILAARV

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994951, Length=388, Percent_Identity=29.8969072164948, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787493, Length=386, Percent_Identity=29.2746113989637, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082107, Length=314, Percent_Identity=32.484076433121, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789163, Length=286, Percent_Identity=31.1188811188811, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789386, Length=366, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537053, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321181, Length=325, Percent_Identity=32.3076923076923, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24657991, Length=294, Percent_Identity=28.9115646258503, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 40848; Mature: 40717

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIHFGAGMFDRLVDFSLPYGRNALLVTGRGSLQRQGYLHSLFNSFSKAGIQYAHIIVD
CCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
HEPSPELIDHAVSCYRDLSIDVVLAVGGGSVIDAGKAIAAMLPSGDAVERYIEGLPGAVP
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
HNGRKVPFIAVPTTSGTGSEVTNNAVISRTGKNGFKRSLRHSSFVPDVAVVDPLLMCSAS
CCCCCCCEEEECCCCCCCCHHHCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
RELTASSGMDACTQLLEAYVSPFATPYTDLLAFQGLEYFARSFLSACSDGADDSAVRADM
CCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AYAALLSGVVLSNAGLGIVHGFASSVGGEYDIPHGMLCATLLAEATRTNIRELRASRNEY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PALKKYAQAGQLFSGRDATDCSEGCNMLIEKLEEWQQQLAIPKLSAYGIGIFDTERLASR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHH
TRSKSNPVDLTLQSMQAILAARV
HCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAIHFGAGMFDRLVDFSLPYGRNALLVTGRGSLQRQGYLHSLFNSFSKAGIQYAHIIVD
CCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
HEPSPELIDHAVSCYRDLSIDVVLAVGGGSVIDAGKAIAAMLPSGDAVERYIEGLPGAVP
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
HNGRKVPFIAVPTTSGTGSEVTNNAVISRTGKNGFKRSLRHSSFVPDVAVVDPLLMCSAS
CCCCCCCEEEECCCCCCCCHHHCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
RELTASSGMDACTQLLEAYVSPFATPYTDLLAFQGLEYFARSFLSACSDGADDSAVRADM
CCHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AYAALLSGVVLSNAGLGIVHGFASSVGGEYDIPHGMLCATLLAEATRTNIRELRASRNEY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PALKKYAQAGQLFSGRDATDCSEGCNMLIEKLEEWQQQLAIPKLSAYGIGIFDTERLASR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHH
TRSKSNPVDLTLQSMQAILAARV
HCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]