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The map label for this gene is pilT [H]

Identifier: 118578975

GI number: 118578975

Start: 574099

End: 575175

Strand: Direct

Name: pilT [H]

Synonym: Ppro_0536

Alternate gene names: 118578975

Gene position: 574099-575175 (Clockwise)

Preceding gene: 118578974

Following gene: 118578976

Centisome position: 14.32

GC content: 62.77

Gene sequence:

>1077_bases
ATGGCACGTATTGACGCACTTTTCAGGATGATGAAAGAGCAGGGGGCATCGGACCTGCACCTCTCCACCGGCAATCCGCC
CATTTTTCGCCTGCATGGCGAGATGGTCCGGCTCAACTTCAAATCCCTGGGGCACGACGAGCTGCACTCGATCCTGTTCG
AGATCTTGAATGCCAAGCAGAAGGAGCAGTTCGAGACGACCAAGGATCTGGACTTCGCCTATGCCGTTCCGGACCTGGCG
CGCTTCCGGGGCAACATCCTCATGCAGCACCGGGGCATCGCCGCGGTGTTCCGCATCATACCGGGCAAGGTCCTCTCCGC
CGACGACCTGAACCTGCCCGACGGGGTGCGGCGCATGACCAATTTCAAGAAGGGGCTGGTGCTGGTCACCGGCCCCACCG
GCTCGGGCAAATCCACCACCCTGGCCGCCCTGATCGACCACATCAACTCCACCCGCAAGGAGCATATCCTCACCCTGGAG
GACCCGCTGGAGTTCATCCACGAGAACAAGCAGTCGCTGCTCAACCAGCGCCAGATCGGCGAGCACACCCAGAGCTTCTC
CGCAGCCCTCAAGGCGGCCCTGCGGGAGGATCCGGACATCATCCTGGTGGGGGAGATGCGCGACCTGGAGACCATTCAGC
TGGCCATGAGCGCCGCCGAGACCGGACACCTGGTGTTCGGCACCCTGCACACCAACACGGCAGCCAAGACCATCGACCGG
ATCATCGACGTCTTTCCCACCGAGCAGCAGCAGCAGGTGCGGGCCATGTTGTCCGAGTCGCTCAGGGGGGTGGTCTGCCA
GCAGCTGCTCAAGACCGCCGAGGGGAAGGGGCGCGTGGCCGCCTTCGAAATCATGCTCGGCACCCCTGCCATCGGCAACC
TGATCCGCGAGGCCAAGACCTTCCAGATCCCCTCCATCATCCAGACCGCCAAGAAGGACGGCATGCAGCTCATGGACCAG
CATCTGCTGGACCTGCTCAAGACCAAGAAGGTCAACCCGGAAGAGGCCTGCCGCTGCGCCATCGACAAGAAGCAGTTCGA
ACAGTACCTGCCGGCTGACAGCGCCAAGGCGGACTAA

Upstream 100 bases:

>100_bases
TGTCGCCCGAGGAGTACGTCTCCTCGGTGCTGATGTAGCGGAGACCCTGTCCGAGCGCTTTCCCGCAGATGACAATTTCG
GGATTGATGGGAGCATATCC

Downstream 100 bases:

>100_bases
GCCCGGAATACGAAGATAACTATGCGAGGAATATTATGAATTTCAATCACTTTGATGAATCAGGCAATGCCGTGATGGTG
GATGTGAGCCACAAACAGCC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQKEQFETTKDLDFAYAVPDLA
RFRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMTNFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLE
DPLEFIHENKQSLLNQRQIGEHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
IIDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKTFQIPSIIQTAKKDGMQLMDQ
HLLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD

Sequences:

>Translated_358_residues
MARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQKEQFETTKDLDFAYAVPDLA
RFRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMTNFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLE
DPLEFIHENKQSLLNQRQIGEHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
IIDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKTFQIPSIIQTAKKDGMQLMDQ
HLLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD
>Mature_357_residues
ARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQKEQFETTKDLDFAYAVPDLAR
FRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMTNFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLED
PLEFIHENKQSLLNQRQIGEHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDRI
IDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKTFQIPSIIQTAKKDGMQLMDQH
LLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=299, Percent_Identity=45.1505016722408, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1789723, Length=154, Percent_Identity=43.5064935064935, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786296, Length=149, Percent_Identity=44.2953020134228, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39868; Mature: 39736

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQ
CCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEEHHHCCHHHHHHHHHHHHCCHH
KEQFETTKDLDFAYAVPDLARFRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMT
HHHHHHHHCCCHHHHCHHHHHHHCCHHHHHCCHHHHHHHCCCCEECCCCCCCCHHHHHHH
NFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLEDPLEFIHENKQSLLNQRQIG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHH
EHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
IIDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHH
FQIPSIIQTAKKDGMQLMDQHLLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQ
CHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEEHHHCCHHHHHHHHHHHHCCHH
KEQFETTKDLDFAYAVPDLARFRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMT
HHHHHHHHCCCHHHHCHHHHHHHCCHHHHHCCHHHHHHHCCCCEECCCCCCCCHHHHHHH
NFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLEDPLEFIHENKQSLLNQRQIG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHH
EHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
IIDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHH
FQIPSIIQTAKKDGMQLMDQHLLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]