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The map label for this gene is epsE [H]

Identifier: 118578974

GI number: 118578974

Start: 572384

End: 574036

Strand: Direct

Name: epsE [H]

Synonym: Ppro_0535

Alternate gene names: 118578974

Gene position: 572384-574036 (Clockwise)

Preceding gene: 118578973

Following gene: 118578975

Centisome position: 14.28

GC content: 65.34

Gene sequence:

>1653_bases
ATGACCGAGTTGACGAAAAAGGGAAGTTTGGGCCAGATCCTGACGGCATCCAGAATCATCAGTGAGATCGATATTCTGGC
CGCGCTGGAGGAACAGGCCCGCAGCGGCTGCCGCTTCGGCGAGGCACTGGTCCGGCTGGGGGTCGCCACCCAGGAGGATG
TGGACTGGGCGCTCTCCAGCCAGCTGGATATTCCCTATATCCGGCTCAAGCGGGAACTGGTCGATCCGGGTGCGATTGCC
CTGGTGCCGGCCGCCATGGCCCGCCGGTTCTCCTGTATCCCGCTCTTCCGCGCCGGCGACGAGCTGAACATCGCCATTGC
CGACCCCCTGAACCGCGCCGCCATTCAGGCCCTGGAACTGGCCACCGGCCTGCGGGTGAGCATCTCCGTTGCCCTGCTGC
GGGAAATCATGGAGATGGTCGACGAATGCTACGGCCCGGCCATGAACGACAGCCTCGGCCTGGAATCGGTGCTTTTTTCC
GGCAGGGTGCTGGAAGCGATCAACGCCGATCTGAGCGGCTCCAAGCTGCTGGACGGCCTGCTGATCACCATCATAAAGAA
CAGGCTCTCCTCCCTCTCCCTGCATCCGAGCGGCTCCCGGGTGCTGATCCGCGGACGACGGCAGGGGGTCTCCTCGGAGA
TCGGTACGCTCTCCGGCGAACACTACCCCGATTTCCTCCAGAGGCTGCGCCTGCGCGCCTCGATCTCCCCCGCCGCGGAC
CCGGTCTCGGAGGGGTGTTTCAGCTTCGACTACCGCTCCCGCAGGGCGCACTTCCGGGTCGCCCTGCTGCTGACGCCCGC
CGGTGACTACGTAACGCTTACGTTGCTGGTTCCGGAGAACTTTCCCGAGCACCTGGCCGACCTGGAGCTGCCCGAAGAAC
AGCTCGGCGCCTTCAGACACCTGGCACGGGCCAAACAGGGTATCACCTTCTTTGCCTCATCCTCTCCCGCGGAGCGTAGC
CATTTCATGGGTCTGATGCTGGAAGAGGGCGGGACCGACGGCAGGAACGTCCTCATCCTCGGGGACTCCCCCGTCTGTCC
CGGCAATCCTTTTCCGCGGATACCGCTCCCCCGGGAGGGCAAGGAGCGCGCCGGCCTGATAACGGCGGCTCTGGATCACG
ACCCGGATATCCTGGTGATCGAGGATGTGGGGGAGGGGCTTGCCTTCAGCGCCGCCTGTCGCGCCGCCATGCGCGGCAAG
CTGGTGCTGGCCGGCCTGGAGAGCAGGGGGAACCGGGATGCCCTGCGCCGGCTTTTGTTCTGCCAGCAGCAGCATTTCCT
GCTGCCGCTCTTCGTCAATGGCCTGGTATCCCTCAGCGGGGTGCTCAGTCTCTGCCCGCAGTGCCGCGCCGAATACACCC
CCTCCCGGGAAGAGCGGGCCGCCCTGCGCCTGGAGCAGGAACCTGCCAGCTTTTTCCGTTCCGCCGGGTGCGATGCCTGC
GGGGACAGCGGATTTCGCGAGCGGCGCTTCCTGATGGATGCGCTGGTCTTTGACGACGAACTGCTGCAGCTCTTTCGCCA
GGCGGCCGATGTTACCTCCCTGGAGGTTTTTATCTCCCGGCGGGGCCGTCAGGGGATCAGGCAGCAGGCTGCGCGGCTGC
TCAAAGAGGGTGAACTGTCGCCCGAGGAGTACGTCTCCTCGGTGCTGATGTAG

Upstream 100 bases:

>100_bases
GGAAGGCTGAGGCGCCCCGGCGGTTCCCGCGGTGAGGGAGTTTTGGCGTAGTGCCAAGGCACATTCTATGGTATGAACAT
CCAGTTACGGGAGAGGAACC

Downstream 100 bases:

>100_bases
CGGAGACCCTGTCCGAGCGCTTTCCCGCAGATGACAATTTCGGGATTGATGGGAGCATATCCATGGCACGTATTGACGCA
CTTTTCAGGATGATGAAAGA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTELTKKGSLGQILTASRIISEIDILAALEEQARSGCRFGEALVRLGVATQEDVDWALSSQLDIPYIRLKRELVDPGAIA
LVPAAMARRFSCIPLFRAGDELNIAIADPLNRAAIQALELATGLRVSISVALLREIMEMVDECYGPAMNDSLGLESVLFS
GRVLEAINADLSGSKLLDGLLITIIKNRLSSLSLHPSGSRVLIRGRRQGVSSEIGTLSGEHYPDFLQRLRLRASISPAAD
PVSEGCFSFDYRSRRAHFRVALLLTPAGDYVTLTLLVPENFPEHLADLELPEEQLGAFRHLARAKQGITFFASSSPAERS
HFMGLMLEEGGTDGRNVLILGDSPVCPGNPFPRIPLPREGKERAGLITAALDHDPDILVIEDVGEGLAFSAACRAAMRGK
LVLAGLESRGNRDALRRLLFCQQQHFLLPLFVNGLVSLSGVLSLCPQCRAEYTPSREERAALRLEQEPASFFRSAGCDAC
GDSGFRERRFLMDALVFDDELLQLFRQAADVTSLEVFISRRGRQGIRQQAARLLKEGELSPEEYVSSVLM

Sequences:

>Translated_550_residues
MTELTKKGSLGQILTASRIISEIDILAALEEQARSGCRFGEALVRLGVATQEDVDWALSSQLDIPYIRLKRELVDPGAIA
LVPAAMARRFSCIPLFRAGDELNIAIADPLNRAAIQALELATGLRVSISVALLREIMEMVDECYGPAMNDSLGLESVLFS
GRVLEAINADLSGSKLLDGLLITIIKNRLSSLSLHPSGSRVLIRGRRQGVSSEIGTLSGEHYPDFLQRLRLRASISPAAD
PVSEGCFSFDYRSRRAHFRVALLLTPAGDYVTLTLLVPENFPEHLADLELPEEQLGAFRHLARAKQGITFFASSSPAERS
HFMGLMLEEGGTDGRNVLILGDSPVCPGNPFPRIPLPREGKERAGLITAALDHDPDILVIEDVGEGLAFSAACRAAMRGK
LVLAGLESRGNRDALRRLLFCQQQHFLLPLFVNGLVSLSGVLSLCPQCRAEYTPSREERAALRLEQEPASFFRSAGCDAC
GDSGFRERRFLMDALVFDDELLQLFRQAADVTSLEVFISRRGRQGIRQQAARLLKEGELSPEEYVSSVLM
>Mature_549_residues
TELTKKGSLGQILTASRIISEIDILAALEEQARSGCRFGEALVRLGVATQEDVDWALSSQLDIPYIRLKRELVDPGAIAL
VPAAMARRFSCIPLFRAGDELNIAIADPLNRAAIQALELATGLRVSISVALLREIMEMVDECYGPAMNDSLGLESVLFSG
RVLEAINADLSGSKLLDGLLITIIKNRLSSLSLHPSGSRVLIRGRRQGVSSEIGTLSGEHYPDFLQRLRLRASISPAADP
VSEGCFSFDYRSRRAHFRVALLLTPAGDYVTLTLLVPENFPEHLADLELPEEQLGAFRHLARAKQGITFFASSSPAERSH
FMGLMLEEGGTDGRNVLILGDSPVCPGNPFPRIPLPREGKERAGLITAALDHDPDILVIEDVGEGLAFSAACRAAMRGKL
VLAGLESRGNRDALRRLLFCQQQHFLLPLFVNGLVSLSGVLSLCPQCRAEYTPSREERAALRLEQEPASFFRSAGCDACG
DSGFRERRFLMDALVFDDELLQLFRQAADVTSLEVFISRRGRQGIRQQAARLLKEGELSPEEYVSSVLM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=468, Percent_Identity=22.6495726495727, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789723, Length=369, Percent_Identity=24.390243902439, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 60195; Mature: 60064

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELTKKGSLGQILTASRIISEIDILAALEEQARSGCRFGEALVRLGVATQEDVDWALSS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHC
QLDIPYIRLKRELVDPGAIALVPAAMARRFSCIPLFRAGDELNIAIADPLNRAAIQALEL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
ATGLRVSISVALLREIMEMVDECYGPAMNDSLGLESVLFSGRVLEAINADLSGSKLLDGL
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHH
LITIIKNRLSSLSLHPSGSRVLIRGRRQGVSSEIGTLSGEHYPDFLQRLRLRASISPAAD
HHHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
PVSEGCFSFDYRSRRAHFRVALLLTPAGDYVTLTLLVPENFPEHLADLELPEEQLGAFRH
HHHHCCEECCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHH
LARAKQGITFFASSSPAERSHFMGLMLEEGGTDGRNVLILGDSPVCPGNPFPRIPLPREG
HHHHHCCCEEEECCCCCHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
KERAGLITAALDHDPDILVIEDVGEGLAFSAACRAAMRGKLVLAGLESRGNRDALRRLLF
CHHCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
CQQQHFLLPLFVNGLVSLSGVLSLCPQCRAEYTPSREERAALRLEQEPASFFRSAGCDAC
HHHCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCC
GDSGFRERRFLMDALVFDDELLQLFRQAADVTSLEVFISRRGRQGIRQQAARLLKEGELS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
PEEYVSSVLM
HHHHHHHHCC
>Mature Secondary Structure 
TELTKKGSLGQILTASRIISEIDILAALEEQARSGCRFGEALVRLGVATQEDVDWALSS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHC
QLDIPYIRLKRELVDPGAIALVPAAMARRFSCIPLFRAGDELNIAIADPLNRAAIQALEL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
ATGLRVSISVALLREIMEMVDECYGPAMNDSLGLESVLFSGRVLEAINADLSGSKLLDGL
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHH
LITIIKNRLSSLSLHPSGSRVLIRGRRQGVSSEIGTLSGEHYPDFLQRLRLRASISPAAD
HHHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
PVSEGCFSFDYRSRRAHFRVALLLTPAGDYVTLTLLVPENFPEHLADLELPEEQLGAFRH
HHHHCCEECCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHH
LARAKQGITFFASSSPAERSHFMGLMLEEGGTDGRNVLILGDSPVCPGNPFPRIPLPREG
HHHHHCCCEEEECCCCCHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
KERAGLITAALDHDPDILVIEDVGEGLAFSAACRAAMRGKLVLAGLESRGNRDALRRLLF
CHHCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
CQQQHFLLPLFVNGLVSLSGVLSLCPQCRAEYTPSREERAALRLEQEPASFFRSAGCDAC
HHHCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCC
GDSGFRERRFLMDALVFDDELLQLFRQAADVTSLEVFISRRGRQGIRQQAARLLKEGELS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
PEEYVSSVLM
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]