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The map label for this gene is zraS [H]

Identifier: 118578927

GI number: 118578927

Start: 510328

End: 511080

Strand: Direct

Name: zraS [H]

Synonym: Ppro_0488

Alternate gene names: 118578927

Gene position: 510328-511080 (Clockwise)

Preceding gene: 118578926

Following gene: 118578933

Centisome position: 12.73

GC content: 59.63

Gene sequence:

>753_bases
ATGAAGAATCCGGCCGATACGTATGGCCAGGGGAGACTCGACGAAGAGCTCCTGAAGCAGCTGGTCCATAATGAAAAGAT
GGCCGAACTGGGCAGGATGTCCGCGGGGATCGTGCACGAACTGAATGCGCCGCTCTCGGTCATCGCCTCGGCGGCTCAGA
TGATCCTGCGCGAGACGGATGTGCCGGAGTTCGTGCGCGAGATGGTCGGACGCATCGGCGTCGAGGCTCAGCGTCTCTCC
CATCTGGCCAGGGGAATGCTCAGTCTGAGCTGTCGAGACTCGACCCTCCAGGAGGTGGACATCAATCTCACCATCCGCTT
TGTCCTTGATTTCATCTCCTACGAGGCGGCCCGGCGCGCAGTCCTGGTCGTGCCGAAACTGGATTTTCGTCTGCCGGTGA
TCCTGTTGGACGGCAACAAGCTCAAGCAGATCCTGCTCAATCTCGTCATGAATGCGTTGCAGGCCATGGAAGATCAAGGG
GGGGTGTTGCGCGTGGAGACCGGCTCTCCGTTGCCGGGCGAGGTCGACATCGTCATCAATGACAATGGTCCGGGGATTCC
CGCCGAGTCCCTGGAAAAGATATTCCAGCCCGGTTTCACCACCAAGAAGGAAGGAGAGGGGACCGGCCTGGGGCTGTTCG
TTACCAGGATGTTGGTGGAACAATTGGGGGGGCGCATTGAGGTGGACAGCCGCATTAGCGAAGGGACCACATTCAGGGTG
ACACTGTCAACGGGGGAGCGGAGCGAGGATTGA

Upstream 100 bases:

>100_bases
CAACATGTAGTGCCATCCTCCTGGTATTCAGCATAACCGGCAGAAGCCGCCCATTCGGGAGGCTTCTGCCCTTTGCATAT
AAACACACACCGGAGTGGAT

Downstream 100 bases:

>100_bases
TTGTCAGACCGGCTCTTCCAGCAGGGGGATGATGACTACTTTCATGCTTCCCCTGCGCCAGAGACCGATCTTCTGTGCGG
CTGCCCGGGAGAGGTCGATG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKNPADTYGQGRLDEELLKQLVHNEKMAELGRMSAGIVHELNAPLSVIASAAQMILRETDVPEFVREMVGRIGVEAQRLS
HLARGMLSLSCRDSTLQEVDINLTIRFVLDFISYEAARRAVLVVPKLDFRLPVILLDGNKLKQILLNLVMNALQAMEDQG
GVLRVETGSPLPGEVDIVINDNGPGIPAESLEKIFQPGFTTKKEGEGTGLGLFVTRMLVEQLGGRIEVDSRISEGTTFRV
TLSTGERSED

Sequences:

>Translated_250_residues
MKNPADTYGQGRLDEELLKQLVHNEKMAELGRMSAGIVHELNAPLSVIASAAQMILRETDVPEFVREMVGRIGVEAQRLS
HLARGMLSLSCRDSTLQEVDINLTIRFVLDFISYEAARRAVLVVPKLDFRLPVILLDGNKLKQILLNLVMNALQAMEDQG
GVLRVETGSPLPGEVDIVINDNGPGIPAESLEKIFQPGFTTKKEGEGTGLGLFVTRMLVEQLGGRIEVDSRISEGTTFRV
TLSTGERSED
>Mature_250_residues
MKNPADTYGQGRLDEELLKQLVHNEKMAELGRMSAGIVHELNAPLSVIASAAQMILRETDVPEFVREMVGRIGVEAQRLS
HLARGMLSLSCRDSTLQEVDINLTIRFVLDFISYEAARRAVLVVPKLDFRLPVILLDGNKLKQILLNLVMNALQAMEDQG
GVLRVETGSPLPGEVDIVINDNGPGIPAESLEKIFQPGFTTKKEGEGTGLGLFVTRMLVEQLGGRIEVDSRISEGTTFRV
TLSTGERSED

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=37.4449339207048, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=31.8777292576419, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790300, Length=242, Percent_Identity=33.4710743801653, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=30.1724137931034, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=25.5474452554745, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 27452; Mature: 27452

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPADTYGQGRLDEELLKQLVHNEKMAELGRMSAGIVHELNAPLSVIASAAQMILRETD
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VPEFVREMVGRIGVEAQRLSHLARGMLSLSCRDSTLQEVDINLTIRFVLDFISYEAARRA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHCCE
VLVVPKLDFRLPVILLDGNKLKQILLNLVMNALQAMEDQGGVLRVETGSPLPGEVDIVIN
EEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEC
DNGPGIPAESLEKIFQPGFTTKKEGEGTGLGLFVTRMLVEQLGGRIEVDSRISEGTTFRV
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEE
TLSTGERSED
EEECCCCCCC
>Mature Secondary Structure
MKNPADTYGQGRLDEELLKQLVHNEKMAELGRMSAGIVHELNAPLSVIASAAQMILRETD
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VPEFVREMVGRIGVEAQRLSHLARGMLSLSCRDSTLQEVDINLTIRFVLDFISYEAARRA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHCCE
VLVVPKLDFRLPVILLDGNKLKQILLNLVMNALQAMEDQGGVLRVETGSPLPGEVDIVIN
EEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEC
DNGPGIPAESLEKIFQPGFTTKKEGEGTGLGLFVTRMLVEQLGGRIEVDSRISEGTTFRV
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEE
TLSTGERSED
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]