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The map label for this gene is amtB [C]

Identifier: 118578926

GI number: 118578926

Start: 508714

End: 510237

Strand: Direct

Name: amtB [C]

Synonym: Ppro_0487

Alternate gene names: 118578926

Gene position: 508714-510237 (Clockwise)

Preceding gene: 118578925

Following gene: 118578927

Centisome position: 12.69

GC content: 63.65

Gene sequence:

>1524_bases
ATGAAAGCTCAAGGATGGCTTTCACACTACTGTTTCATTCCCCGGATGGAGCAGCTTGCCGTCGTACTGATCCTCGGCAT
TACGCTGTTTCTGTCTCCCTTTGTCGCATCGGCCCCGGCTGAAGAGACGTCGGCCACTGCTCCCGCGGCAACCTCCTCTG
CCGCTCAAGGCGCGGCAGCCGCGAACTCTCCGTCCGCCGAAGCCGTGTCGGTCGTCGCTCCTGCCGAGCCCCCCGATGTC
GCTCCGGTGCTCGACACCGGCGACACCGCCTGGATGCTTGTCTCCTCTGCCCTGGTGCTGCTGATGATCCCCGGCTTGGC
GCTCTTCTACGGCGGCATGGTCCGCCAGAAGAACGTGCTCTCCACCATGATGCATTCCCTGGTGGCCATGGGCATTGTCG
GCGTGCAGTGGGCGGTGATCGGCTATTCCCTTGCCTTTGCTCCCGACATGGGGGGCATGGGGCTGGTCGGAGACTTCAGC
AAGGCCATGCTGGACGGCCTGGTTATCGTCAAGGACGCTGCCAGCGGCACGGTTGACTGGGCCCTGTTCCAGAATTACAC
CAAGGAGCCGGGCGCGATTCCCGAGCTGGTGTTTGCCATGTACCAGTGCATGTTCGCCATGATCACCGTGGCGCTGATTT
CCGGCGCCATGGCGGAGCGGGTCAAGTTTTCGGCCTACTGCCTGTTCGTGCTGCTCTGGACCACCCTGGTGTACGATCCG
CTGGCACACTGGGTCTGGATGACCGAAGGCTGGCTGTTCAAGAAGGGGGCGCTTGATTTCGCCGGTGGCACGGTTGTTCA
CCTCTCCTCCGGTATTTCGGCCTTGGTGTTTCTGAAGTTCCTGGGCAAACGCCACGGATTTCCCCATGAGCGCATGGCGC
CCCACAACCTGCCCCTGACACTCTTGGGCGTGGGTCTGCTCTGGTTCGGCTGGTTCGGATTCAACGCCGGTTCGGCCATC
GTGGGCGTCAACAACTCCGACGCGGCCGGTGGCCTGGCAGGTCTGGCCTTCGCCACCACCACCATAGCGCCGGCAGCAGC
CGGTCTCTCCTGGATGATCGCCGAGTGGATCCACTCAGGGCGCCCCAGCGCCCTCGGCTTCGGCTCCGGTGTCGTGGCCG
GCCTGGTGGTCATCACGCCGGCAGCCGGGTTCGTGCAGCCGGGAGCCGCCCTGCTGATGGGGCTTATGGCCGGTGTGGTC
TGCTACGGCGGCGTGCTGATGAAGGCCAAGCTGAAGTACGACGACTCCCTGGACGCCTTCGGTGTTCACGGCGTGGGCGG
TACCTTCGGCGCCATCCTGACCGGCGTGTTCGCCACGGTGGGCGCCACCGGCCTCCTGGCCGGCAACGCCCAGCAGCTGA
TGACCCAGCTGATCGCCATCCTGGCGGCAGGCGCCTATGCCGTCATCGTAACCCTGATCATATCCTTTGTTCTCGACAAG
ACCATTGGTCTGCGGGTGGAGAAGGAAGACGAGATCATGGGTCTGGACCAGACACAGCATTCCGAGTCGGGCTACAACAT
GTAG

Upstream 100 bases:

>100_bases
AAATAATCTGTTCCTGCACGAGGGGCCGGCCCCATCGCCATGATGGTGGCCGGACTCTCGTTTTCCGCACCCACACACCA
CTTATCGAAGGAGATACTGC

Downstream 100 bases:

>100_bases
TGCCATCCTCCTGGTATTCAGCATAACCGGCAGAAGCCGCCCATTCGGGAGGCTTCTGCCCTTTGCATATAAACACACAC
CGGAGTGGATATGAAGAATC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKAQGWLSHYCFIPRMEQLAVVLILGITLFLSPFVASAPAEETSATAPAATSSAAQGAAAANSPSAEAVSVVAPAEPPDV
APVLDTGDTAWMLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSLVAMGIVGVQWAVIGYSLAFAPDMGGMGLVGDFS
KAMLDGLVIVKDAASGTVDWALFQNYTKEPGAIPELVFAMYQCMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDP
LAHWVWMTEGWLFKKGALDFAGGTVVHLSSGISALVFLKFLGKRHGFPHERMAPHNLPLTLLGVGLLWFGWFGFNAGSAI
VGVNNSDAAGGLAGLAFATTTIAPAAAGLSWMIAEWIHSGRPSALGFGSGVVAGLVVITPAAGFVQPGAALLMGLMAGVV
CYGGVLMKAKLKYDDSLDAFGVHGVGGTFGAILTGVFATVGATGLLAGNAQQLMTQLIAILAAGAYAVIVTLIISFVLDK
TIGLRVEKEDEIMGLDQTQHSESGYNM

Sequences:

>Translated_507_residues
MKAQGWLSHYCFIPRMEQLAVVLILGITLFLSPFVASAPAEETSATAPAATSSAAQGAAAANSPSAEAVSVVAPAEPPDV
APVLDTGDTAWMLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSLVAMGIVGVQWAVIGYSLAFAPDMGGMGLVGDFS
KAMLDGLVIVKDAASGTVDWALFQNYTKEPGAIPELVFAMYQCMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDP
LAHWVWMTEGWLFKKGALDFAGGTVVHLSSGISALVFLKFLGKRHGFPHERMAPHNLPLTLLGVGLLWFGWFGFNAGSAI
VGVNNSDAAGGLAGLAFATTTIAPAAAGLSWMIAEWIHSGRPSALGFGSGVVAGLVVITPAAGFVQPGAALLMGLMAGVV
CYGGVLMKAKLKYDDSLDAFGVHGVGGTFGAILTGVFATVGATGLLAGNAQQLMTQLIAILAAGAYAVIVTLIISFVLDK
TIGLRVEKEDEIMGLDQTQHSESGYNM
>Mature_507_residues
MKAQGWLSHYCFIPRMEQLAVVLILGITLFLSPFVASAPAEETSATAPAATSSAAQGAAAANSPSAEAVSVVAPAEPPDV
APVLDTGDTAWMLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSLVAMGIVGVQWAVIGYSLAFAPDMGGMGLVGDFS
KAMLDGLVIVKDAASGTVDWALFQNYTKEPGAIPELVFAMYQCMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDP
LAHWVWMTEGWLFKKGALDFAGGTVVHLSSGISALVFLKFLGKRHGFPHERMAPHNLPLTLLGVGLLWFGWFGFNAGSAI
VGVNNSDAAGGLAGLAFATTTIAPAAAGLSWMIAEWIHSGRPSALGFGSGVVAGLVVITPAAGFVQPGAALLMGLMAGVV
CYGGVLMKAKLKYDDSLDAFGVHGVGGTFGAILTGVFATVGATGLLAGNAQQLMTQLIAILAAGAYAVIVTLIISFVLDK
TIGLRVEKEDEIMGLDQTQHSESGYNM

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=432, Percent_Identity=43.75, Blast_Score=312, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17550182, Length=454, Percent_Identity=30.8370044052863, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=29.3064876957494, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=456, Percent_Identity=26.7543859649123, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=450, Percent_Identity=27.5555555555556, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=37.1681415929204, Blast_Score=265, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=33.6384439359268, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321559, Length=444, Percent_Identity=32.4324324324324, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI161078314, Length=435, Percent_Identity=32.4137931034483, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52767; Mature: 52767

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]