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The map label for this gene is glgX [H]

Identifier: 118578861

GI number: 118578861

Start: 437849

End: 439939

Strand: Direct

Name: glgX [H]

Synonym: Ppro_0419

Alternate gene names: 118578861

Gene position: 437849-439939 (Clockwise)

Preceding gene: 118578860

Following gene: 118578865

Centisome position: 10.92

GC content: 66.38

Gene sequence:

>2091_bases
ATGAGCGCGGCCGATACCAGGCCGGGCCGGGGACGGAGCCATCCGCTGGGGGCCACAATCGTTCCGGGCGGAGTGAATTT
CAGTCTCTTCTCCCGGGACTGCAGCGCCGTCCAGCTGCTCCTGTTCGACCGGGTTGATGACGGCAGGCCGGCGCGCGTCA
TAATGCTGGATCCGCGGAAGAACCGCAGCTACCACTACTGGCACCTGTTCATTCCCGGCCTGGGTCCGGGTCAGCTGTAC
GGCTACCGGGTCGCCGGTCCGCGCGGACCCGGCGGGGGGGGGCGCTTCGACCCGGACAAGCTGCTCCTGGATCCCTACGG
CAGGGGGGTGGCTGTCCCTGCCGGCTACAGCCGCGCAGCCGCCATGGCGCCGGGTGACAACTGCGGCCTGGCCATGAAGA
GCGTGGTGGCCGACCCGCGCGGGTACGACTGGGAGGGGGACGCCCCCCTGGAGCGCCCCTTCTCCCGAACCCTGATCTAT
GAGCTGCACCTGGCCGGCTTCACCCGTCACCCTTCCTCGGGCGTCACCCCCGACAAGCGCGGCACCTACGCCGGACTGGT
GGAGAAGATTCCCTACCTGCAGCAACTGGGGGTTACCGCCGTGGAACTCCTGCCGGTGTTCCAGTTCGATCCCCAGGACG
CCCCCCCGGGGTTCAGCAACTACTGGGGGTACAGCCCGGTCTCCTTCTTCGCCCCCCACGCGGCCTACAGCTCCCGCCAA
GACCCGCTGGGGCCGCTGGACGAATTCCGCGATATGGTCAAGGCGCTCCACCGGGCCGGCATCGAGGTGGTCCTGGATGT
GGTCTTCAATCACACCGCCGAGGGGGACCAAGGGGGAGCCACCATCTGCTACCGGGGCCTGGCCAATGATTTCTACTACA
TGCTGGAGCCGGACGGCATCAGCTACGCCAACTACTCCGGCTGCGGCAACACCTTCAACGCCAACAATCCCATCGCCCGG
CGGCTGATTATTGACAGCCTGCACCACTGGGTCAGGGAGATGCACGTGGACGGTTTCCGCTTCGACCTGGCCTCCATCCT
CTCCCGGGACGAGCAGGGCCGCCCCCTGGAGAATCCGCCCCTGCTGTGGGATATCGAAACCGACCCTGCCCTAGCCGGGA
TCAAGCTGATCGCCGAGGCCTGGGACGCGGCCGGGCTCTACCAGGTGGGCAGCTTCATCGGAGACAGCTGGAAGGAGTGG
AACGGCAGGTTCCGGGACGACGTGCGCAGCTTCCTGCGCGGCGACCGCGGAACGGTCTCGCGCTTTGCCTCCCGGCTGCT
TGCCAGCCCCGATATCTATGGCCACGAGGAGCGGGAAGCGGAGCAGAGCATCAACTTCGTCACCTGCCACGACGGCTTCA
CCCTCAACGACCTGGTCTCCTTTAACCGCAAGCATAATCAGGCCAATGGTGAGGATAACCGGGACGGCAGCAATGAGAAC
CTGAGTTGGAACTGCGGCCAGGAGGGGCCCAGCAGTGATCCGTCGGTGGAGGCGCTGCGCAACCGCCAAGTGAAGAACTT
TCTGGCGGTGACCCTGCTGGCCCTGGGCACCCCCATGCTCCTGATGGGGGACGAGGTGCGCAGGACGCAGGGGGGGAACA
ACAATGCCTACTGCCAGGACAACGAGACCGGCTGGTTCGACTGGCGGCTTTTGGAACGCTACGCCGACGTGCACCGCTTT
GCGCGCATGCTGATAGCGGCACGCCAGCGACGCGACCTGGCGCTTGAGGATCCGGGGCTGACCCTCAACCAGCTCCTGGG
CCAGGCGCGCCTGGAATGGCATGGCGTCAGGGCAGGACAGCCGGACTGGGGGGATGACTCCCACAGCATCGCCCTGACGG
CCTGGAGTCAGAGCGGACGGTTCGCTATTCATCTTATGGTGAATGCCTGGCGGGAAGCGCTGGTCTTCCAACTCCCACCC
GCCCCGGACGTGCCGGGGGGCTGCTGGCGCCGTTGGTTGGACACATCCCTGGCGTCGCCGGACGACATCGTAGCGCTGGA
AGATGCTCCCCCCATGGAGAGGGCATGCTACCCGTTGCCACCCCACTCGCTGGCTGCCATGCTTGCCGCTGCTCCCAGCT
GTGCCGTTTAA

Upstream 100 bases:

>100_bases
AGAACGGCGTCGTCTACCAGAGGGACCTGGGCAAGGAGAGCGCCAGGCTCGCGGCGTCCATGACGTTCTTCAACCCGGAC
CCTAGCTGGAAGAAGGTGGA

Downstream 100 bases:

>100_bases
GGTCGCCAGGCACTTCACGGAGAAAGGAGCACCCAAGGGGTGCAACCATGCAACAGGGCACTGGCAATATCTGATTGTGA
AGGCCGTCGCTTTGGGCGGG

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSAADTRPGRGRSHPLGATIVPGGVNFSLFSRDCSAVQLLLFDRVDDGRPARVIMLDPRKNRSYHYWHLFIPGLGPGQLY
GYRVAGPRGPGGGGRFDPDKLLLDPYGRGVAVPAGYSRAAAMAPGDNCGLAMKSVVADPRGYDWEGDAPLERPFSRTLIY
ELHLAGFTRHPSSGVTPDKRGTYAGLVEKIPYLQQLGVTAVELLPVFQFDPQDAPPGFSNYWGYSPVSFFAPHAAYSSRQ
DPLGPLDEFRDMVKALHRAGIEVVLDVVFNHTAEGDQGGATICYRGLANDFYYMLEPDGISYANYSGCGNTFNANNPIAR
RLIIDSLHHWVREMHVDGFRFDLASILSRDEQGRPLENPPLLWDIETDPALAGIKLIAEAWDAAGLYQVGSFIGDSWKEW
NGRFRDDVRSFLRGDRGTVSRFASRLLASPDIYGHEEREAEQSINFVTCHDGFTLNDLVSFNRKHNQANGEDNRDGSNEN
LSWNCGQEGPSSDPSVEALRNRQVKNFLAVTLLALGTPMLLMGDEVRRTQGGNNNAYCQDNETGWFDWRLLERYADVHRF
ARMLIAARQRRDLALEDPGLTLNQLLGQARLEWHGVRAGQPDWGDDSHSIALTAWSQSGRFAIHLMVNAWREALVFQLPP
APDVPGGCWRRWLDTSLASPDDIVALEDAPPMERACYPLPPHSLAAMLAAAPSCAV

Sequences:

>Translated_696_residues
MSAADTRPGRGRSHPLGATIVPGGVNFSLFSRDCSAVQLLLFDRVDDGRPARVIMLDPRKNRSYHYWHLFIPGLGPGQLY
GYRVAGPRGPGGGGRFDPDKLLLDPYGRGVAVPAGYSRAAAMAPGDNCGLAMKSVVADPRGYDWEGDAPLERPFSRTLIY
ELHLAGFTRHPSSGVTPDKRGTYAGLVEKIPYLQQLGVTAVELLPVFQFDPQDAPPGFSNYWGYSPVSFFAPHAAYSSRQ
DPLGPLDEFRDMVKALHRAGIEVVLDVVFNHTAEGDQGGATICYRGLANDFYYMLEPDGISYANYSGCGNTFNANNPIAR
RLIIDSLHHWVREMHVDGFRFDLASILSRDEQGRPLENPPLLWDIETDPALAGIKLIAEAWDAAGLYQVGSFIGDSWKEW
NGRFRDDVRSFLRGDRGTVSRFASRLLASPDIYGHEEREAEQSINFVTCHDGFTLNDLVSFNRKHNQANGEDNRDGSNEN
LSWNCGQEGPSSDPSVEALRNRQVKNFLAVTLLALGTPMLLMGDEVRRTQGGNNNAYCQDNETGWFDWRLLERYADVHRF
ARMLIAARQRRDLALEDPGLTLNQLLGQARLEWHGVRAGQPDWGDDSHSIALTAWSQSGRFAIHLMVNAWREALVFQLPP
APDVPGGCWRRWLDTSLASPDDIVALEDAPPMERACYPLPPHSLAAMLAAAPSCAV
>Mature_695_residues
SAADTRPGRGRSHPLGATIVPGGVNFSLFSRDCSAVQLLLFDRVDDGRPARVIMLDPRKNRSYHYWHLFIPGLGPGQLYG
YRVAGPRGPGGGGRFDPDKLLLDPYGRGVAVPAGYSRAAAMAPGDNCGLAMKSVVADPRGYDWEGDAPLERPFSRTLIYE
LHLAGFTRHPSSGVTPDKRGTYAGLVEKIPYLQQLGVTAVELLPVFQFDPQDAPPGFSNYWGYSPVSFFAPHAAYSSRQD
PLGPLDEFRDMVKALHRAGIEVVLDVVFNHTAEGDQGGATICYRGLANDFYYMLEPDGISYANYSGCGNTFNANNPIARR
LIIDSLHHWVREMHVDGFRFDLASILSRDEQGRPLENPPLLWDIETDPALAGIKLIAEAWDAAGLYQVGSFIGDSWKEWN
GRFRDDVRSFLRGDRGTVSRFASRLLASPDIYGHEEREAEQSINFVTCHDGFTLNDLVSFNRKHNQANGEDNRDGSNENL
SWNCGQEGPSSDPSVEALRNRQVKNFLAVTLLALGTPMLLMGDEVRRTQGGNNNAYCQDNETGWFDWRLLERYADVHRFA
RMLIAARQRRDLALEDPGLTLNQLLGQARLEWHGVRAGQPDWGDDSHSIALTAWSQSGRFAIHLMVNAWREALVFQLPPA
PDVPGGCWRRWLDTSLASPDDIVALEDAPPMERACYPLPPHSLAAMLAAAPSCAV

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=586, Percent_Identity=44.19795221843, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789839, Length=333, Percent_Identity=27.3273273273273, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77021; Mature: 76890

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAADTRPGRGRSHPLGATIVPGGVNFSLFSRDCSAVQLLLFDRVDDGRPARVIMLDPRK
CCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHEEHHHHCCCCCCCEEEEEECCCC
NRSYHYWHLFIPGLGPGQLYGYRVAGPRGPGGGGRFDPDKLLLDPYGRGVAVPAGYSRAA
CCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCE
AMAPGDNCGLAMKSVVADPRGYDWEGDAPLERPFSRTLIYELHLAGFTRHPSSGVTPDKR
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCC
GTYAGLVEKIPYLQQLGVTAVELLPVFQFDPQDAPPGFSNYWGYSPVSFFAPHAAYSSRQ
CCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHCCHHHHCCCC
DPLGPLDEFRDMVKALHRAGIEVVLDVVFNHTAEGDQGGATICYRGLANDFYYMLEPDGI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCC
SYANYSGCGNTFNANNPIARRLIIDSLHHWVREMHVDGFRFDLASILSRDEQGRPLENPP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
LLWDIETDPALAGIKLIAEAWDAAGLYQVGSFIGDSWKEWNGRFRDDVRSFLRGDRGTVS
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCHHH
RFASRLLASPDIYGHEEREAEQSINFVTCHDGFTLNDLVSFNRKHNQANGEDNRDGSNEN
HHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
LSWNCGQEGPSSDPSVEALRNRQVKNFLAVTLLALGTPMLLMGDEVRRTQGGNNNAYCQD
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCEEECC
NETGWFDWRLLERYADVHRFARMLIAARQRRDLALEDPGLTLNQLLGQARLEWHGVRAGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCC
PDWGDDSHSIALTAWSQSGRFAIHLMVNAWREALVFQLPPAPDVPGGCWRRWLDTSLASP
CCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCC
DDIVALEDAPPMERACYPLPPHSLAAMLAAAPSCAV
CCEEEECCCCCHHHCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAADTRPGRGRSHPLGATIVPGGVNFSLFSRDCSAVQLLLFDRVDDGRPARVIMLDPRK
CCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHEEHHHHCCCCCCCEEEEEECCCC
NRSYHYWHLFIPGLGPGQLYGYRVAGPRGPGGGGRFDPDKLLLDPYGRGVAVPAGYSRAA
CCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCE
AMAPGDNCGLAMKSVVADPRGYDWEGDAPLERPFSRTLIYELHLAGFTRHPSSGVTPDKR
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCC
GTYAGLVEKIPYLQQLGVTAVELLPVFQFDPQDAPPGFSNYWGYSPVSFFAPHAAYSSRQ
CCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHCCHHHHCCCC
DPLGPLDEFRDMVKALHRAGIEVVLDVVFNHTAEGDQGGATICYRGLANDFYYMLEPDGI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCC
SYANYSGCGNTFNANNPIARRLIIDSLHHWVREMHVDGFRFDLASILSRDEQGRPLENPP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
LLWDIETDPALAGIKLIAEAWDAAGLYQVGSFIGDSWKEWNGRFRDDVRSFLRGDRGTVS
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCHHH
RFASRLLASPDIYGHEEREAEQSINFVTCHDGFTLNDLVSFNRKHNQANGEDNRDGSNEN
HHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
LSWNCGQEGPSSDPSVEALRNRQVKNFLAVTLLALGTPMLLMGDEVRRTQGGNNNAYCQD
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCEEECC
NETGWFDWRLLERYADVHRFARMLIAARQRRDLALEDPGLTLNQLLGQARLEWHGVRAGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCC
PDWGDDSHSIALTAWSQSGRFAIHLMVNAWREALVFQLPPAPDVPGGCWRRWLDTSLASP
CCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCC
DDIVALEDAPPMERACYPLPPHSLAAMLAAAPSCAV
CCEEEECCCCCHHHCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]