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The map label for this gene is hycD [H]

Identifier: 118578779

GI number: 118578779

Start: 338086

End: 338994

Strand: Direct

Name: hycD [H]

Synonym: Ppro_0337

Alternate gene names: 118578779

Gene position: 338086-338994 (Clockwise)

Preceding gene: 118578778

Following gene: 118578780

Centisome position: 8.44

GC content: 65.02

Gene sequence:

>909_bases
ATGACGAACAGCCTGATTCACATCCTGCTGGCGCTGATCATGCCGCCGCTCCTGCTGGGGGTGATCGCCAAGACCAAGGC
GGCCTTTGCCGGCCGGCTCGGTGCGCCCTTCCTGCAGCCGTACTACGACATCCTCCGTCTGCTGCGCAAGGGAAGCGTAT
TCAGCACCACCACCACCTGGATCTTCCGCGCTGGTCCCATCGTGGGCCTGGCAACGACCCTGGTAGCCAGCCTGCTGGTC
CCTTTCGGCGCCCATCCCGCGCCGATCTCCTTCCAGGGGGACATGATCCTCTACGCCTACCTGTTTGCCCTGGGGCGCTT
CTTCACCATGGCCGCCGCCCTTGACACCGGCTCCAGCTTCGAGGGGATGGGCACGGCCCGCGAGGCCACCTACTCCTGCC
TGGCCGAACCGACCCTCTTCTTCGCCCTGATCACCCTCTCCCGGCTCTCGGGGAGTTACTCCCTGACCGGTATGCTCTCC
GGCCTGGATCAGCGGCTCTGGCTGAATGCCGGCGCCGCCATGCTGCTGTTGATCGGCGGCATGTTCATCGTTCTCTTGGC
TGAAAACTGTCGCATCCCCTTCGACGACCCCACCACCCACCTGGAGCTGACCATGATCCACGAGGTGATGGTGCTGGACC
ACAGCGGCCCGGCCTTCGGACTGATCCTGTACGGGGCGGCGCTGAAGCTGTTCGTGCTGGGCGCCTTCTTCATGCACCTG
GCGCTCCCCTTCACCAGCGCTAACCCCCTGGCCGACTGGGGGATCTTCGTCGTTTCCATGCTGCTCTTGGCGGTCGCCAT
CGGCGTGGTGGAATCGGTCATGGCCCGCCTCAGGCTGGTCCGGGTTCCGCAACTGCTGGTGGCCGCAACCATCCTGGCCG
CGTTTTCCACCCTGCTCGTCATCAGGTGA

Upstream 100 bases:

>100_bases
GCGCCTGCAGCACGGCCGGATGCATGTGTACATGATCTATATCTTTGCCACGCTGCTGATCCTGATGCTCTGGGCACACT
GAACCAATCACGGGGGGCTT

Downstream 100 bases:

>100_bases
CTCCATGTTCTCATTACCCGACCAGCTGCTCGTCCTCGTCCTGTTGATCAACTTCATCACCATCGGCACCAGCCGCCTGG
TCTTCTCCATCCGCGCCGTT

Product: NAD-dependent dehydrogenase subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTNSLIHILLALIMPPLLLGVIAKTKAAFAGRLGAPFLQPYYDILRLLRKGSVFSTTTTWIFRAGPIVGLATTLVASLLV
PFGAHPAPISFQGDMILYAYLFALGRFFTMAAALDTGSSFEGMGTAREATYSCLAEPTLFFALITLSRLSGSYSLTGMLS
GLDQRLWLNAGAAMLLLIGGMFIVLLAENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMHL
ALPFTSANPLADWGIFVVSMLLLAVAIGVVESVMARLRLVRVPQLLVAATILAAFSTLLVIR

Sequences:

>Translated_302_residues
MTNSLIHILLALIMPPLLLGVIAKTKAAFAGRLGAPFLQPYYDILRLLRKGSVFSTTTTWIFRAGPIVGLATTLVASLLV
PFGAHPAPISFQGDMILYAYLFALGRFFTMAAALDTGSSFEGMGTAREATYSCLAEPTLFFALITLSRLSGSYSLTGMLS
GLDQRLWLNAGAAMLLLIGGMFIVLLAENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMHL
ALPFTSANPLADWGIFVVSMLLLAVAIGVVESVMARLRLVRVPQLLVAATILAAFSTLLVIR
>Mature_301_residues
TNSLIHILLALIMPPLLLGVIAKTKAAFAGRLGAPFLQPYYDILRLLRKGSVFSTTTTWIFRAGPIVGLATTLVASLLVP
FGAHPAPISFQGDMILYAYLFALGRFFTMAAALDTGSSFEGMGTAREATYSCLAEPTLFFALITLSRLSGSYSLTGMLSG
LDQRLWLNAGAAMLLLIGGMFIVLLAENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMHLA
LPFTSANPLADWGIFVVSMLLLAVAIGVVESVMARLRLVRVPQLLVAATILAAFSTLLVIR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=288, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082115, Length=248, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 32469; Mature: 32338

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSLIHILLALIMPPLLLGVIAKTKAAFAGRLGAPFLQPYYDILRLLRKGSVFSTTTTW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
IFRAGPIVGLATTLVASLLVPFGAHPAPISFQGDMILYAYLFALGRFFTMAAALDTGSSF
HEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGTAREATYSCLAEPTLFFALITLSRLSGSYSLTGMLSGLDQRLWLNAGAAMLLLIGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
MFIVLLAENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMHL
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPFTSANPLADWGIFVVSMLLLAVAIGVVESVMARLRLVRVPQLLVAATILAAFSTLLV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IR
CC
>Mature Secondary Structure 
TNSLIHILLALIMPPLLLGVIAKTKAAFAGRLGAPFLQPYYDILRLLRKGSVFSTTTTW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
IFRAGPIVGLATTLVASLLVPFGAHPAPISFQGDMILYAYLFALGRFFTMAAALDTGSSF
HEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGTAREATYSCLAEPTLFFALITLSRLSGSYSLTGMLSGLDQRLWLNAGAAMLLLIGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
MFIVLLAENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMHL
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPFTSANPLADWGIFVVSMLLLAVAIGVVESVMARLRLVRVPQLLVAATILAAFSTLLV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]