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The map label for this gene is hyfB [H]

Identifier: 118578778

GI number: 118578778

Start: 336070

End: 338067

Strand: Direct

Name: hyfB [H]

Synonym: Ppro_0336

Alternate gene names: 118578778

Gene position: 336070-338067 (Clockwise)

Preceding gene: 118578775

Following gene: 118578779

Centisome position: 8.38

GC content: 64.41

Gene sequence:

>1998_bases
ATGTTTTATGCTCCGCAGGTGGGGGGCGACCCTGCATTCCTGGCGATAGTTGCCGCGCTTGCCATGGGCTGCGCCGGTCT
CCCCGGCCTGTTCATGCGCACACCCGGCCCCGGACAGATCCTGGCCACGGGTATCACGATCCTGGCCAGCCTGGCCGGCA
TTCCGGCAGCGCTGATGCTGCTGTTCAGCGACAGGGTCCCGGCCTATGTTCTTGAATGGGGACTCCCCTTCGGACCGTGC
GAACTGGCCATCGATCCGCTCTCGGCCTTTTTCCTGCTGCCGGTCTTTCTGGTGGCCGGGGCGGGATCGCTCTTTGCCCT
GGGGTACTGGCCTGCCACAAAGAATCGCGGCAGCGAGCCCATGCTGACGTTTTTTTACGGCCTTCTGGCCTCATCGATGG
CGATACTGCTCATGGCGCGTAACAGCGCCTTTTTTTTGATGGTATGGGAGGTCATGGCGCTGTCGGCCTATTTCCTGCTG
GTAACGGAGCATGGCCGGGAGGACGTGCGGCGGGCCGGAATCGTCTATCTGGTTGCCACCCATTGCGGCACCGCCGCCCT
GTTCGTCTACTTCTCCCTGCTGGCCAGCAGCAGCGGCTCGTTCCTCCTCCCCCCTGCCGGCTCGCTCTCCGCGCTGCCGG
CCGCCTCCCTGGCCATTGCCCTGGCGGCCTTCATCGGCTTCGGCGCCAAGGGGGGCATCATGCCGCTGCACATCTGGCTC
CCATCGGCCCACGCCAACGCACCCAGCCACGTCTCCGCCATGATGTCCGGGGTCATGCTCAAGATGGGGCTCTACGGCAT
CTTCCGCACCCTGACCTTTTTCCGCGACCCGCCGCTCTCCTGGGGAGCCTTTCTGGCCCTGACCGGAATCGCCTCGGCCC
TGCTGGGCATCTCCTTCGCCGTTGCCCAGTGTGACCTGAAGCGCCTACTGGCCTGCAGCAGCATCGAGAATATCGGCATC
ATCACCACTGGCCTGGGGGTGGCCATGATGGGCATCTCCAGCCACAACACGACCCTGGCCTACCTGGGCATGGCGGGCGC
CATGCTGCACATCCTCAACCACAGCCTGTTCAAACCGCTGCTCTTCCTCGGTTCCGGTGCCATCATCCACGCCAGCGGAA
CGCGCGACATGAACCTGATGGGAGGGCTGGCCAAGGGGATGCCGAAGACGGCCTTCCTGGTTCTGGTCGGCTCCCTGGCC
ATCTGCGGCATTCCCCCCCTGAACGGTTTTGTCAGTGAATTCCTGCTCTACCTGGGCTTCTTCTCCCAGCTCCAGGCCGC
AAGCCTGACCTTCCTGGTGCTGTTGGCGCCGCTCCTGGCGCTGGTGGGGGGGCTGGCGCTGATTGCCTTTACCAAGCTGT
ACTGCTGCATTTTCCTCGGTGTACCCCGCAATGTGGAGGCCGGCCATACCCATGAGGCGGGTCCTGCCATGCTGATTCCC
CTGGCGCTCCTGGCGCTGCTCTGCCTGCTGGTCGGTCTCTTGCCCCAGTACGGGCTGCGCCTGGTTGAACCGGCCCTGGC
GCTGTTCGTTCCGCCCGGGGCGAGCGCCACCCTCTCCCTGGGCTATGCCTTGACCCTAGAGCAGCTCTCGCAGCTTGGGC
TGTTCCTGCTGCTGGTTTCGGCCCTGGTGTTCTTTTTTTGGCGCATGCTCTTGGCGCGGGCGCCCCAGGCCAGCGGACCC
ACCTGGGGATGCGGCTATCAGCGGGGAACGGCGCGGATCCAGTACACGGCCACATCGTTTTCCGAGTACGCCGTTTCCGT
CTTCAACGGTTTCATCCGCCAGCGCATCCAGCGTCCCGCCCTGGCGGGGCTCTTTCCGCTGACGGCCGACTGCGTTGACC
GGCCCACGGAGACAGTGCTGGACCGGATCATCGCTCCGCTGTTCACCCTGGCCGGGCTGCTGTTCGCTTTTCTGATGCGC
CTGCAGCACGGCCGGATGCATGTGTACATGATCTATATCTTTGCCACGCTGCTGATCCTGATGCTCTGGGCACACTGA

Upstream 100 bases:

>100_bases
CTCATACGGCGCTGGAGGTTCACTATTCACATTTACCCGTTGCTGTGAGTCTTTTCCAGAAAAACACTCTATGTATCATC
CTTTCCAAGGAGCTCCTATC

Downstream 100 bases:

>100_bases
ACCAATCACGGGGGGCTTATGACGAACAGCCTGATTCACATCCTGCTGGCGCTGATCATGCCGCCGCTCCTGCTGGGGGT
GATCGCCAAGACCAAGGCGG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MFYAPQVGGDPAFLAIVAALAMGCAGLPGLFMRTPGPGQILATGITILASLAGIPAALMLLFSDRVPAYVLEWGLPFGPC
ELAIDPLSAFFLLPVFLVAGAGSLFALGYWPATKNRGSEPMLTFFYGLLASSMAILLMARNSAFFLMVWEVMALSAYFLL
VTEHGREDVRRAGIVYLVATHCGTAALFVYFSLLASSSGSFLLPPAGSLSALPAASLAIALAAFIGFGAKGGIMPLHIWL
PSAHANAPSHVSAMMSGVMLKMGLYGIFRTLTFFRDPPLSWGAFLALTGIASALLGISFAVAQCDLKRLLACSSIENIGI
ITTGLGVAMMGISSHNTTLAYLGMAGAMLHILNHSLFKPLLFLGSGAIIHASGTRDMNLMGGLAKGMPKTAFLVLVGSLA
ICGIPPLNGFVSEFLLYLGFFSQLQAASLTFLVLLAPLLALVGGLALIAFTKLYCCIFLGVPRNVEAGHTHEAGPAMLIP
LALLALLCLLVGLLPQYGLRLVEPALALFVPPGASATLSLGYALTLEQLSQLGLFLLLVSALVFFFWRMLLARAPQASGP
TWGCGYQRGTARIQYTATSFSEYAVSVFNGFIRQRIQRPALAGLFPLTADCVDRPTETVLDRIIAPLFTLAGLLFAFLMR
LQHGRMHVYMIYIFATLLILMLWAH

Sequences:

>Translated_665_residues
MFYAPQVGGDPAFLAIVAALAMGCAGLPGLFMRTPGPGQILATGITILASLAGIPAALMLLFSDRVPAYVLEWGLPFGPC
ELAIDPLSAFFLLPVFLVAGAGSLFALGYWPATKNRGSEPMLTFFYGLLASSMAILLMARNSAFFLMVWEVMALSAYFLL
VTEHGREDVRRAGIVYLVATHCGTAALFVYFSLLASSSGSFLLPPAGSLSALPAASLAIALAAFIGFGAKGGIMPLHIWL
PSAHANAPSHVSAMMSGVMLKMGLYGIFRTLTFFRDPPLSWGAFLALTGIASALLGISFAVAQCDLKRLLACSSIENIGI
ITTGLGVAMMGISSHNTTLAYLGMAGAMLHILNHSLFKPLLFLGSGAIIHASGTRDMNLMGGLAKGMPKTAFLVLVGSLA
ICGIPPLNGFVSEFLLYLGFFSQLQAASLTFLVLLAPLLALVGGLALIAFTKLYCCIFLGVPRNVEAGHTHEAGPAMLIP
LALLALLCLLVGLLPQYGLRLVEPALALFVPPGASATLSLGYALTLEQLSQLGLFLLLVSALVFFFWRMLLARAPQASGP
TWGCGYQRGTARIQYTATSFSEYAVSVFNGFIRQRIQRPALAGLFPLTADCVDRPTETVLDRIIAPLFTLAGLLFAFLMR
LQHGRMHVYMIYIFATLLILMLWAH
>Mature_665_residues
MFYAPQVGGDPAFLAIVAALAMGCAGLPGLFMRTPGPGQILATGITILASLAGIPAALMLLFSDRVPAYVLEWGLPFGPC
ELAIDPLSAFFLLPVFLVAGAGSLFALGYWPATKNRGSEPMLTFFYGLLASSMAILLMARNSAFFLMVWEVMALSAYFLL
VTEHGREDVRRAGIVYLVATHCGTAALFVYFSLLASSSGSFLLPPAGSLSALPAASLAIALAAFIGFGAKGGIMPLHIWL
PSAHANAPSHVSAMMSGVMLKMGLYGIFRTLTFFRDPPLSWGAFLALTGIASALLGISFAVAQCDLKRLLACSSIENIGI
ITTGLGVAMMGISSHNTTLAYLGMAGAMLHILNHSLFKPLLFLGSGAIIHASGTRDMNLMGGLAKGMPKTAFLVLVGSLA
ICGIPPLNGFVSEFLLYLGFFSQLQAASLTFLVLLAPLLALVGGLALIAFTKLYCCIFLGVPRNVEAGHTHEAGPAMLIP
LALLALLCLLVGLLPQYGLRLVEPALALFVPPGASATLSLGYALTLEQLSQLGLFLLLVSALVFFFWRMLLARAPQASGP
TWGCGYQRGTARIQYTATSFSEYAVSVFNGFIRQRIQRPALAGLFPLTADCVDRPTETVLDRIIAPLFTLAGLLFAFLMR
LQHGRMHVYMIYIFATLLILMLWAH

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=490, Percent_Identity=26.3265306122449, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI251831116, Length=272, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788827, Length=607, Percent_Identity=38.2207578253707, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI2367154, Length=563, Percent_Identity=31.2611012433393, Blast_Score=192, Evalue=8e-50,
Organism=Escherichia coli, GI1788831, Length=364, Percent_Identity=34.0659340659341, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788613, Length=381, Percent_Identity=28.0839895013123, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788829, Length=411, Percent_Identity=26.2773722627737, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1788614, Length=472, Percent_Identity=29.8728813559322, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70649; Mature: 70649

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYAPQVGGDPAFLAIVAALAMGCAGLPGLFMRTPGPGQILATGITILASLAGIPAALML
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LFSDRVPAYVLEWGLPFGPCELAIDPLSAFFLLPVFLVAGAGSLFALGYWPATKNRGSEP
HHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCH
MLTFFYGLLASSMAILLMARNSAFFLMVWEVMALSAYFLLVTEHGREDVRRAGIVYLVAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHCCEEEEEEH
HCGTAALFVYFSLLASSSGSFLLPPAGSLSALPAASLAIALAAFIGFGAKGGIMPLHIWL
HCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEE
PSAHANAPSHVSAMMSGVMLKMGLYGIFRTLTFFRDPPLSWGAFLALTGIASALLGISFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VAQCDLKRLLACSSIENIGIITTGLGVAMMGISSHNTTLAYLGMAGAMLHILNHSLFKPL
HHHHHHHHHHHHHCCCCCCEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFLGSGAIIHASGTRDMNLMGGLAKGMPKTAFLVLVGSLAICGIPPLNGFVSEFLLYLGF
HHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FSQLQAASLTFLVLLAPLLALVGGLALIAFTKLYCCIFLGVPRNVEAGHTHEAGPAMLIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LALLALLCLLVGLLPQYGLRLVEPALALFVPPGASATLSLGYALTLEQLSQLGLFLLLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALVFFFWRMLLARAPQASGPTWGCGYQRGTARIQYTATSFSEYAVSVFNGFIRQRIQRPA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCH
LAGLFPLTADCVDRPTETVLDRIIAPLFTLAGLLFAFLMRLQHGRMHVYMIYIFATLLIL
HHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MLWAH
HHHCC
>Mature Secondary Structure
MFYAPQVGGDPAFLAIVAALAMGCAGLPGLFMRTPGPGQILATGITILASLAGIPAALML
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LFSDRVPAYVLEWGLPFGPCELAIDPLSAFFLLPVFLVAGAGSLFALGYWPATKNRGSEP
HHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCH
MLTFFYGLLASSMAILLMARNSAFFLMVWEVMALSAYFLLVTEHGREDVRRAGIVYLVAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHCCEEEEEEH
HCGTAALFVYFSLLASSSGSFLLPPAGSLSALPAASLAIALAAFIGFGAKGGIMPLHIWL
HCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEE
PSAHANAPSHVSAMMSGVMLKMGLYGIFRTLTFFRDPPLSWGAFLALTGIASALLGISFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VAQCDLKRLLACSSIENIGIITTGLGVAMMGISSHNTTLAYLGMAGAMLHILNHSLFKPL
HHHHHHHHHHHHHCCCCCCEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFLGSGAIIHASGTRDMNLMGGLAKGMPKTAFLVLVGSLAICGIPPLNGFVSEFLLYLGF
HHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FSQLQAASLTFLVLLAPLLALVGGLALIAFTKLYCCIFLGVPRNVEAGHTHEAGPAMLIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LALLALLCLLVGLLPQYGLRLVEPALALFVPPGASATLSLGYALTLEQLSQLGLFLLLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALVFFFWRMLLARAPQASGPTWGCGYQRGTARIQYTATSFSEYAVSVFNGFIRQRIQRPA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCH
LAGLFPLTADCVDRPTETVLDRIIAPLFTLAGLLFAFLMRLQHGRMHVYMIYIFATLLIL
HHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MLWAH
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]