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The map label for this gene is pheS

Identifier: 162382771

GI number: 162382771

Start: 4364447

End: 4365481

Strand: Reverse

Name: pheS

Synonym: BALH_4142

Alternate gene names: 162382771

Gene position: 4365481-4364447 (Counterclockwise)

Preceding gene: 118479709

Following gene: 118479707

Centisome position: 83.04

GC content: 39.9

Gene sequence:

>1035_bases
ATGGAAGCACGTTTAAAAGAGCTAAAGCAAAAAGCGTTAGAGCTTATTGAAGAAGCGAAAGAGCTAAAAGGCTTAAACGA
CGTACGCGTAGCGTATTTAGGAAAAAAAGGCCCAATTACAGAAGTATTACGCGGCATGGGAAAATTATCAGCAGAAGAGC
GTCCACGTATGGGAGCATTAGTAAATGAAGTACGTGAAGCGATTCAAACGCGTCTAGATGACAAAATCAGTAACTTAGAA
AAAGCAGTAATTGAAGCGAAATTAGCTACTGAAACAATTGATGTTACATTGCCAGGTCGTCCTGTTGAAACAGGTTGTCA
CCATCCGTTAACAGCTGTTGTTGAACAAATTGAAGATGTATTCATCGGTATGGGTTATGAAGTAGCTGAAGGAACAGAAG
TAGAAAAAGACTACTACAACTTCGAAGCATTAAACTTACCGAAAGATCACCCAGCGCGTGATATGCAAGATACATTCTAC
ATTACAGAAGAAACGTTATTACGTACACATACATCTTCTGTGCAAGCACGTACGATGGAAAATAATAAAGAAAAAGGCCC
AATTAAAATTATTTGTCCTGGTAAAGTGTATCGCCGTGATGACGATGATGCGACGCATTCACACCAGTTCATGCAAATTG
AAGGTCTTGTAATCGATAAAAACATTCGCATGAGTGACTTAAAAGGTACACTGCAAGTATTCGTGAAAAAGATGTTCGGT
GAAGATCGTGAAATCCGTCTTCGTCCAAGTTTCTTCCCATTCACAGAGCCATCTGTAGAGATGGATATTTCTTGTATGAT
GTGTCACGGCAAAGGTTGCGGCACTTGTAAAGGAACTGGTTGGATTGAAATTTTAGGTGCAGGTATGGTTCACCCGAACG
TACTTGAAATGGCTGGTTATGATTCAAAAGAATATCAAGGTTTCGCATTCGGTATGGGCGCAGAGCGTATCGCAATGTTG
AAATACGGCGTAGATGACATTCGTCATTTCTATACAAATGATGTACGTTTCTTACAACAATTCAAACGAGCGTAA

Upstream 100 bases:

>100_bases
TGGGCAGACATGTTCTGTCAATTTAGGGTGGTACCGCGAATTTACCTCGTCCCTTTTTGGGAGCGAGGTTTTTTTATTTT
TAAAATTAGGAGGGTTCCAA

Downstream 100 bases:

>100_bases
GGGAAGGAGAGGATAAGTATGTTCGTATCATATCGTTGGTTACAAGAGTATGTAGATATTAAAGATGTAACAGCACAAGA
ACTAGCAGACAAAATCACGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MEARLKELKQKALELIEEAKELKGLNDVRVAYLGKKGPITEVLRGMGKLSAEERPRMGALVNEVREAIQTRLDDKISNLE
KAVIEAKLATETIDVTLPGRPVETGCHHPLTAVVEQIEDVFIGMGYEVAEGTEVEKDYYNFEALNLPKDHPARDMQDTFY
ITEETLLRTHTSSVQARTMENNKEKGPIKIICPGKVYRRDDDDATHSHQFMQIEGLVIDKNIRMSDLKGTLQVFVKKMFG
EDREIRLRPSFFPFTEPSVEMDISCMMCHGKGCGTCKGTGWIEILGAGMVHPNVLEMAGYDSKEYQGFAFGMGAERIAML
KYGVDDIRHFYTNDVRFLQQFKRA

Sequences:

>Translated_344_residues
MEARLKELKQKALELIEEAKELKGLNDVRVAYLGKKGPITEVLRGMGKLSAEERPRMGALVNEVREAIQTRLDDKISNLE
KAVIEAKLATETIDVTLPGRPVETGCHHPLTAVVEQIEDVFIGMGYEVAEGTEVEKDYYNFEALNLPKDHPARDMQDTFY
ITEETLLRTHTSSVQARTMENNKEKGPIKIICPGKVYRRDDDDATHSHQFMQIEGLVIDKNIRMSDLKGTLQVFVKKMFG
EDREIRLRPSFFPFTEPSVEMDISCMMCHGKGCGTCKGTGWIEILGAGMVHPNVLEMAGYDSKEYQGFAFGMGAERIAML
KYGVDDIRHFYTNDVRFLQQFKRA
>Mature_344_residues
MEARLKELKQKALELIEEAKELKGLNDVRVAYLGKKGPITEVLRGMGKLSAEERPRMGALVNEVREAIQTRLDDKISNLE
KAVIEAKLATETIDVTLPGRPVETGCHHPLTAVVEQIEDVFIGMGYEVAEGTEVEKDYYNFEALNLPKDHPARDMQDTFY
ITEETLLRTHTSSVQARTMENNKEKGPIKIICPGKVYRRDDDDATHSHQFMQIEGLVIDKNIRMSDLKGTLQVFVKKMFG
EDREIRLRPSFFPFTEPSVEMDISCMMCHGKGCGTCKGTGWIEILGAGMVHPNVLEMAGYDSKEYQGFAFGMGAERIAML
KYGVDDIRHFYTNDVRFLQQFKRA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=312, Percent_Identity=29.8076923076923, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4758340, Length=276, Percent_Identity=28.9855072463768, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1788007, Length=340, Percent_Identity=50.2941176470588, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=29.1970802919708, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=29.1970802919708, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=245, Percent_Identity=34.2857142857143, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=272, Percent_Identity=30.1470588235294, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=271, Percent_Identity=28.4132841328413, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=30.6569343065693, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137424, Length=293, Percent_Identity=26.962457337884, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BACAA (C3PAE9)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868839.1
- ProteinModelPortal:   C3PAE9
- SMR:   C3PAE9
- EnsemblBacteria:   EBBACT00000129490
- GeneID:   7851429
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4814
- GeneTree:   EBGT00050000002461
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38976; Mature: 38976

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARLKELKQKALELIEEAKELKGLNDVRVAYLGKKGPITEVLRGMGKLSAEERPRMGAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
VNEVREAIQTRLDDKISNLEKAVIEAKLATETIDVTLPGRPVETGCHHPLTAVVEQIEDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
FIGMGYEVAEGTEVEKDYYNFEALNLPKDHPARDMQDTFYITEETLLRTHTSSVQARTME
HHCCCEEECCCCCCHHHHCCCEEECCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHH
NNKEKGPIKIICPGKVYRRDDDDATHSHQFMQIEGLVIDKNIRMSDLKGTLQVFVKKMFG
CCCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHC
EDREIRLRPSFFPFTEPSVEMDISCMMCHGKGCGTCKGTGWIEILGAGMVHPNVLEMAGY
CCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCC
DSKEYQGFAFGMGAERIAMLKYGVDDIRHFYTNDVRFLQQFKRA
CCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEARLKELKQKALELIEEAKELKGLNDVRVAYLGKKGPITEVLRGMGKLSAEERPRMGAL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
VNEVREAIQTRLDDKISNLEKAVIEAKLATETIDVTLPGRPVETGCHHPLTAVVEQIEDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
FIGMGYEVAEGTEVEKDYYNFEALNLPKDHPARDMQDTFYITEETLLRTHTSSVQARTME
HHCCCEEECCCCCCHHHHCCCEEECCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHH
NNKEKGPIKIICPGKVYRRDDDDATHSHQFMQIEGLVIDKNIRMSDLKGTLQVFVKKMFG
CCCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHC
EDREIRLRPSFFPFTEPSVEMDISCMMCHGKGCGTCKGTGWIEILGAGMVHPNVLEMAGY
CCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCC
DSKEYQGFAFGMGAERIAMLKYGVDDIRHFYTNDVRFLQQFKRA
CCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA