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The map label for this gene is ytzG [H]

Identifier: 118479842

GI number: 118479842

Start: 4503769

End: 4504506

Strand: Reverse

Name: ytzG [H]

Synonym: BALH_4280

Alternate gene names: 118479842

Gene position: 4504506-4503769 (Counterclockwise)

Preceding gene: 118479843

Following gene: 118479841

Centisome position: 85.68

GC content: 34.96

Gene sequence:

>738_bases
GTGAAAAACGTGAGATTAGATAAATTATTAGCAAATATGGGATATGGTAGTAGAAAAGAAGTAAAGAAATTATTGAAAGA
CGGCGTTGTGAAAATTGATGGAATATCGGTGAAAGATGCAAAGGTTCATGTAAATGTAGAAGAACAAGAAGTTATGATTC
ACGGTGAAGTTGTGGAATATAAAGAGTTTGTTTACTTAATGATGCATAAACCACAAGGCGTTATTTCGGCGACAGAAGAT
GATAATCATGAAACGGTAATAGATTTATTAGAGTTAGAGGATGCGATTTTTAATCCGTTCCCAGTTGGAAGACTTGATAT
TGATACAGAAGGTTTCTTATTAATAACAAATGACGGGAAGTTATCACATCAATTGTTATCTCCGAAAAAGCATGTGCCGA
AAAAATATTATGCACACGTTGCAGGAGTAGTAACAGAAGAGGATGTAAAAGAGTTTGCTAAAGGTGTTATTTTAGATGAT
GGCTATGAGACAAAGCCAGGTGCACTTACAATATTAAAAAGCGATGATATTTCTGAAATCGAGCTTGTCATTACAGAAGG
AAAGTTCCATCAAGTGAAGCGTATGTTTGAAGCGGTAGGGAAAAAAGTAGTCTACTTAAAGAGAACAGAGATGGGACCGT
TAGTGTTAGATGAAGAGCTAGAACTTGGACAATACCGAGAGTTAACAGATGAAGAAGTAGAGATGTTAAAAACATATCAA
GTAGACAATGAGAAATAG

Upstream 100 bases:

>100_bases
CGTTAGAGAAAATCCAGCGTAAACTTGGCAATAGATTCAAAATAAAGTTGAAATCAAAAGGGGCGTAATTACGTCCCCTT
TTCTATGATAAACAAAGAAG

Downstream 100 bases:

>100_bases
TGCTATTTTTAGGCGCAGAAAAGACCCCTTTCTAATGAGTTAGAAAGGGGTATGTATTTATTTACATACAATTATTTTTC
GTTATGAGGTCATTCTCACT

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKNVRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATED
DNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDD
GYETKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQ
VDNEK

Sequences:

>Translated_245_residues
MKNVRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATED
DNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDD
GYETKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQ
VDNEK
>Mature_245_residues
MKNVRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATED
DNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDD
GYETKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQ
VDNEK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=37.5, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=32.3886639676113, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=30.0847457627119, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=30.939226519337, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27859; Mature: 27859

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEY
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCEEECCEEEEEECHHEEEEECHHHHH
KEFVYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGK
HHEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCC
LSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEI
HHHHHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEE
ELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQ
EEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCHHHCCCHHHHHHHHHHC
VDNEK
CCCCC
>Mature Secondary Structure
MKNVRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEY
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCEEECCEEEEEECHHEEEEECHHHHH
KEFVYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGK
HHEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCC
LSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEI
HHHHHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEE
ELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQ
EEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCHHHCCCHHHHHHHHHHC
VDNEK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]