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The map label for this gene is pps [H]

Identifier: 118478414

GI number: 118478414

Start: 2941054

End: 2942001

Strand: Reverse

Name: pps [H]

Synonym: BALH_2780

Alternate gene names: 118478414

Gene position: 2942001-2941054 (Counterclockwise)

Preceding gene: 118478416

Following gene: 118478413

Centisome position: 55.96

GC content: 39.35

Gene sequence:

>948_bases
ATGTACTACTGCTACAATCAAGAACACATGCATAGTAAGGAGAGATGTTACATGAGTTCTTTCGTTCTCGATTTTCAGGA
AATAGAAAAAACGCAGCTTTCACTTGTTGGTGGAAAAGGTTTGAATTTAGGGGCGTTATCTAACATGCAAGAGATACAAG
TGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGCAAAATGAAGGGCTTCAAGCTTTGTTGCAA
CAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGCAAGAAGATTAGAGAAGTTATTATGGCGGT
ACAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTTTGGAAATGAACATGCGTATGCCGTGCGCT
CTAGTGCTACGGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAGATACGTATTTAAATATTATCGGAGAAAAC
GCAATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGATCGTGCAGTTATGTACCGCATGCAAAATGGTTT
TGAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTCCCGCAGGCTTCAGGAATTTTATTTACCGCTG
ATCCGATTACTTCTAACCGGAAAGTGTTATCAATCGATGCCAGTTTTGGACTTGGCGAGGCATTAGTATCGGGACTCGTC
TCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATTGCAACTAAAAAATTAGCTATTTATGCTTT
AAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAATTCAAACACTATCTGACCAACAAATATTAC
AACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAGATATTGAATGGTGTTAA

Upstream 100 bases:

>100_bases
TGTAATGGAAGTGAATTAGAGGGGGAGAAATAAAAATAATTAACTCTTCCTAACTTAATTATACCACGTAAATAAAGTTC
GAAATAGACTGTTTCTTCTC

Downstream 100 bases:

>100_bases
GTCGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAGAAAATGATGAGGGAAA
TCATGTGTATATATCAGTTG

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MYYCYNQEHMHSKERCYMSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQALLQ
QLTKLKREERVRIGEISKKIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGEN
AILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLV
SADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWC

Sequences:

>Translated_315_residues
MYYCYNQEHMHSKERCYMSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQALLQ
QLTKLKREERVRIGEISKKIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGEN
AILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLV
SADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWC
>Mature_315_residues
MYYCYNQEHMHSKERCYMSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCVTTAGYEKAIEQNEGLQALLQ
QLTKLKREERVRIGEISKKIREVIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGEN
AILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLV
SADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWC

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=317, Percent_Identity=33.4384858044164, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17564524, Length=305, Percent_Identity=30.1639344262295, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 35352; Mature: 35352

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYCYNQEHMHSKERCYMSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCV
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCHHHCCCCCEEE
TTAGYEKAIEQNEGLQALLQQLTKLKREERVRIGEISKKIREVIMAVQIPSDVVEAVAHY
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVM
HHHHCCCCEEEEECCCCHHHCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
YRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLV
HHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
SADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGR
CCCCCEECCHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
QIEAYFGCPQDIEWC
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MYYCYNQEHMHSKERCYMSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQEIQVPEGFCV
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCHHHCCCCCEEE
TTAGYEKAIEQNEGLQALLQQLTKLKREERVRIGEISKKIREVIMAVQIPSDVVEAVAHY
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVM
HHHHCCCCEEEEECCCCHHHCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
YRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLV
HHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
SADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGR
CCCCCEECCHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
QIEAYFGCPQDIEWC
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]