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The map label for this gene is ppsA [H]

Identifier: 118478413

GI number: 118478413

Start: 2939344

End: 2940969

Strand: Reverse

Name: ppsA [H]

Synonym: BALH_2779

Alternate gene names: 118478413

Gene position: 2940969-2939344 (Counterclockwise)

Preceding gene: 118478414

Following gene: 118478411

Centisome position: 55.94

GC content: 38.25

Gene sequence:

>1626_bases
GTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTTATCTTTTTTCTTGTTAACGAC
TAATGCTCCTATGCGTAAAGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAGCTTTACCTGCTAGCAGAGATT
ATTTAATAAACACTTTAGGGAAATCGGATCCACTTGTAAGAGATGCATTAACGACTATAATCGAGCGAGATGATTTTATT
ACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAGTAAAAGTAAGCCACCAGTAAGTTCGCAACCAGAAATCGAAAA
TGATCAAGCAATCGTTACGAATTTAATAAAGAATAGTGAAGCATCAATCGAAGAGTTAAAACGAAACATGCAAACGAAAT
CAGAGGTGGATGTACTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATTATTTAACCCGCAAAGTATAGCT
GTCATCATGGCAGGTATGAACGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCTAGGTGAAAAGAATGCAGCAGA
CACGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGGATGTTGCTGATGCAATTCGTC
CGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAACTTGTTCAGTATAAAGGCGGA
GAAAAAGCTCGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGGCGAAATCGACATTACGAAAAC
GCGCTGGAGTGAGAAGCCAACAACCATTATCCCAATGATATTAAATAATATAAGAGACTTTGAAGCGGGTGCTAGTAAGC
AGAAGTTTGAAAAAGGACTGCAGGAAGCTTTGAATAAAGAAGAAGAGTTATTAGACCGATTGCAACATTTGCCGAAGGGT
AAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGTAATATCCGTAATTTTATTGGCTATCGTGAATATCCAAAATACGG
CATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGAAAGAAGCTGAGCAACTTGTACAAAGCGGTGTCATTTGTG
AGGTCGATGATATATACTATTTAACTTTTGAAGAACTTCATGAAGTTGTTCGCACGAATAAACTAGATTACGAGCTTATC
CAAAAACAAAAAAATGATTATAAATTATATGAAAAATTAGCACCTCCTCGTATAATGACGTCTGATGGAGAAATCATTAC
AGGTAAATATAAACGAGAAAACCTCCCAGCTGACGCGATTGTAGGTCTGCCTGTTTCTTCCGGAGTAGTTGAGGGGAGAG
CCCGCGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTGGTTACGGCATTTACGGACCCTGGCTGG
ACGCCGTTGTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGCGGGCTCATGACACACGGAGCAGTAATCGCACGTGA
ATATGGCTTACCAGCAGTCGTCGGGGTGGAGAATGCTACAAAGCTAATAAAAGACGGACAACGAATTCGGGTACATGGAA
CAGAAGGGTATATTGAAATGTTGTAA

Upstream 100 bases:

>100_bases
TATTGAATGGTGTTAAGTCGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAG
AAAATGATGAGGGAAATCAT

Downstream 100 bases:

>100_bases
TGAAACAAAGGATCATCCGCTATTATAGTGGATGATCCTTTATTATGTAGTTGACTTATTTCTTTAACCCAGCCGCATAA
AAAGGGCCATGAACTGCAAT

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTIIERDDFI
TLLPDEEKEKSASKSKPPVSSQPEIENDQAIVTNLIKNSEASIEELKRNMQTKSEVDVLDFILEDIQQLKKVLFNPQSIA
VIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGG
EKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKG
KQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELI
QKQKNDYKLYEKLAPPRIMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGW
TPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEML

Sequences:

>Translated_541_residues
MYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTIIERDDFI
TLLPDEEKEKSASKSKPPVSSQPEIENDQAIVTNLIKNSEASIEELKRNMQTKSEVDVLDFILEDIQQLKKVLFNPQSIA
VIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGG
EKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKG
KQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELI
QKQKNDYKLYEKLAPPRIMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGW
TPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEML
>Mature_541_residues
MYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLALPASRDYLINTLGKSDPLVRDALTTIIERDDFI
TLLPDEEKEKSASKSKPPVSSQPEIENDQAIVTNLIKNSEASIEELKRNMQTKSEVDVLDFILEDIQQLKKVLFNPQSIA
VIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGG
EKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKG
KQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELI
QKQKNDYKLYEKLAPPRIMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGW
TPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEML

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=180, Percent_Identity=33.8888888888889, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=324, Percent_Identity=29.6296296296296, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 61113; Mature: 61113

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLALPASRDYLINTLG
CEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEEECHHHHCCCCCCHHHHHHCC
KSDPLVRDALTTIIERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDQAIVTNLIKNSE
CCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
ASIEELKRNMQTKSEVDVLDFILEDIQQLKKVLFNPQSIAVIMAGMNASTWINEKMEQWL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
GEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCC
EKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGL
CHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHH
QEALNKEEELLDRLQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALL
HHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELIQKQKNDYKLYEKLAPPRIMT
HHHHHHHHCCCEEEECCEEEEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCEEE
SDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGW
CCCCEEECCCCCCCCCCCCEEECCCCCCHHCCCEEEEEEEHHCCCCCCCEEEEEECCCCC
TPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCC
L
C
>Mature Secondary Structure
MYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLALPASRDYLINTLG
CEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEEECHHHHCCCCCCHHHHHHCC
KSDPLVRDALTTIIERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDQAIVTNLIKNSE
CCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
ASIEELKRNMQTKSEVDVLDFILEDIQQLKKVLFNPQSIAVIMAGMNASTWINEKMEQWL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
GEKNAADTLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCC
EKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGL
CHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHH
QEALNKEEELLDRLQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALL
HHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELIQKQKNDYKLYEKLAPPRIMT
HHHHHHHHCCCEEEECCEEEEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCEEE
SDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGW
CCCCEEECCCCCCCCCCCCEEECCCCCCHHCCCEEEEEEEHHCCCCCCCEEEEEECCCCC
TPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]