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The map label for this gene is hly [H]

Identifier: 118477929

GI number: 118477929

Start: 2439530

End: 2440780

Strand: Direct

Name: hly [H]

Synonym: BALH_2275

Alternate gene names: 118477929

Gene position: 2439530-2440780 (Clockwise)

Preceding gene: 118477928

Following gene: 118477930

Centisome position: 46.4

GC content: 32.93

Gene sequence:

>1251_bases
GTGGCTGTCTGTATGAAAAAAGCAAATGGAATAGCTAAAAGTGTTGCGGTAGCGTCGGTTATTATGTCTGGATCACTTGG
TTTACAAGCAACATCTGCATTTGCAGATTCTAAAGGAACTGTAGAAAATCTTCAAAATGGTGGAAAAGTCTATAATAGTT
TTAAAACTACTTATGACATGAAGCAGAATATTAAAAATTCAATAAAGGTTTCTTTTATTGAGGATCCTTACGCAGATAAA
AAGATTGCAATTGTTACCACTGATGGTAGTAATATAGATGCGAAATATACAATTAATGGCGGATACTATAACGCAGGTTT
AAAATGGCCATCGGCGTATCATACGGAAGCAGAAATAACAAGTGGTGATAGCGCTCAATTTCATAAAGCTGCCCCAGTGA
ATACAATGACTTCAGCAAAGGTTACTTCTGAAGTAGGATATACACTTGGCGGAAGTGTGAAAGTTGGGGTAAATGATAAA
GGGCCAAATGCCGATGCGAGTATTACAGGTAGCTTTGCTTGGAAAGAAAGTGTATCTTACGATCAAGTAGACTATAAAAC
GGTATTAGAGACTCATACAGATAAAAAATTGAATTGGAAAGTAGGATTCCAATCGTTTAATTATCCGGAGTGGGGAATTT
ATAATCGTGATTCATTTAATACTTTCTATGGTAATCAACTATTTATGAAATCACGTAGTTATAATGAAGGAACGAATAAT
TTTGTTTCAAAAGATACAGTACCTGCTTTAACAGGATATGGTTTTTCTCCTAATGTAGTAGCAGTTATTACTGCAGATAA
AACAGAATCTACATCTGATTTAAAAATAACAAATCGTAGAATATCAGATCAGTACAATATCGAATGGGTAAGTTCGAAAT
GGTGGGGAACAAATAATAAAGATACATATAATGAGTTCTTTACAAACAACTATAAATTAGACTGGAAAAACCATCAAGTT
ACTCTTGATAACCAAAAAGCCCTTGAAGAACAAATAAGTAGTATCAAAAATGTGAATAACCAACTTAACAAAGGAAAAGG
GAAATTATCTTTTTCAATGAATGGAAACCAACTCAAAGCGACATCTAGCAATGCTGGTTATGGTATTAGTTACGAGGATA
AAAATTGGGGTATCTTTGTGAATGGTGAAAAGATCTATACGTTTAATGAAAAAACAACTGTAGGTAATATTTCGAATGAC
ATTAACAAATTAAACATCAAAGGACCTTATATTGAGATTAAAAAAATCTAA

Upstream 100 bases:

>100_bases
ATAAAGATTAATAAATTTCTACATTGTAACGTTATGAATCTATCTTTTGTATTGAGAGCTAAAAATAATACGTTTATATC
TTTTAAATGTTTAAAAAGGA

Downstream 100 bases:

>100_bases
TCAGTAAATAAGGTACAATTGATGGATAATTATTTCATCAAAATATGAGTATAAAGGTTTATTTTAAATCTGTATAACGT
AATGAAAGGACCAGATAAAC

Product: hemolysin II

Products: NA

Alternate protein names: Alpha-HL; Alpha-toxin [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAVCMKKANGIAKSVAVASVIMSGSLGLQATSAFADSKGTVENLQNGGKVYNSFKTTYDMKQNIKNSIKVSFIEDPYADK
KIAIVTTDGSNIDAKYTINGGYYNAGLKWPSAYHTEAEITSGDSAQFHKAAPVNTMTSAKVTSEVGYTLGGSVKVGVNDK
GPNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNYPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNN
FVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNKDTYNEFFTNNYKLDWKNHQV
TLDNQKALEEQISSIKNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKNWGIFVNGEKIYTFNEKTTVGNISND
INKLNIKGPYIEIKKI

Sequences:

>Translated_416_residues
MAVCMKKANGIAKSVAVASVIMSGSLGLQATSAFADSKGTVENLQNGGKVYNSFKTTYDMKQNIKNSIKVSFIEDPYADK
KIAIVTTDGSNIDAKYTINGGYYNAGLKWPSAYHTEAEITSGDSAQFHKAAPVNTMTSAKVTSEVGYTLGGSVKVGVNDK
GPNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNYPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNN
FVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNKDTYNEFFTNNYKLDWKNHQV
TLDNQKALEEQISSIKNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKNWGIFVNGEKIYTFNEKTTVGNISND
INKLNIKGPYIEIKKI
>Mature_415_residues
AVCMKKANGIAKSVAVASVIMSGSLGLQATSAFADSKGTVENLQNGGKVYNSFKTTYDMKQNIKNSIKVSFIEDPYADKK
IAIVTTDGSNIDAKYTINGGYYNAGLKWPSAYHTEAEITSGDSAQFHKAAPVNTMTSAKVTSEVGYTLGGSVKVGVNDKG
PNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNYPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNNF
VSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNKDTYNEFFTNNYKLDWKNHQVT
LDNQKALEEQISSIKNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKNWGIFVNGEKIYTFNEKTTVGNISNDI
NKLNIKGPYIEIKKI

Specific function: Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005831
- InterPro:   IPR003963
- InterPro:   IPR001340
- InterPro:   IPR016183 [H]

Pfam domain/function: PF07968 Leukocidin [H]

EC number: NA

Molecular weight: Translated: 46101; Mature: 45969

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVCMKKANGIAKSVAVASVIMSGSLGLQATSAFADSKGTVENLQNGGKVYNSFKTTYDM
CEEEEECCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHHHCCCCEEHHHHHHHHH
KQNIKNSIKVSFIEDPYADKKIAIVTTDGSNIDAKYTINGGYYNAGLKWPSAYHTEAEIT
HHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCEEEC
SGDSAQFHKAAPVNTMTSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSY
CCCCCCEECCCCCCCCCCCHHHHHCCEEECCEEEEECCCCCCCCCCEEEEEEEECCCCCC
DQVDYKTVLETHTDKKLNWKVGFQSFNYPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNN
CCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCC
FVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNK
CCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCEEEECEECCCCCCEEEEECCCCCCCCC
DTYNEFFTNNYKLDWKNHQVTLDNQKALEEQISSIKNVNNQLNKGKGKLSFSMNGNQLKA
CCHHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEE
TSSNAGYGISYEDKNWGIFVNGEKIYTFNEKTTVGNISNDINKLNIKGPYIEIKKI
ECCCCCCEEEEECCCCEEEECCCEEEEECCCCEECCCCCCCEEEEECCCEEEEEEC
>Mature Secondary Structure 
AVCMKKANGIAKSVAVASVIMSGSLGLQATSAFADSKGTVENLQNGGKVYNSFKTTYDM
EEEEECCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHHHCCCCEEHHHHHHHHH
KQNIKNSIKVSFIEDPYADKKIAIVTTDGSNIDAKYTINGGYYNAGLKWPSAYHTEAEIT
HHHCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCEEEC
SGDSAQFHKAAPVNTMTSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSY
CCCCCCEECCCCCCCCCCCHHHHHCCEEECCEEEEECCCCCCCCCCEEEEEEEECCCCCC
DQVDYKTVLETHTDKKLNWKVGFQSFNYPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNN
CCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCC
FVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNK
CCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCEEEECEECCCCCCEEEEECCCCCCCCC
DTYNEFFTNNYKLDWKNHQVTLDNQKALEEQISSIKNVNNQLNKGKGKLSFSMNGNQLKA
CCHHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEE
TSSNAGYGISYEDKNWGIFVNGEKIYTFNEKTTVGNISNDINKLNIKGPYIEIKKI
ECCCCCCEEEEECCCCEEEECCCEEEEECCCCEECCCCCCCEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6500704; 1587866; 8943190; 1400487; 8168947; 8188346; 7559447 [H]