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The map label for this gene is amiA [C]

Identifier: 118477928

GI number: 118477928

Start: 2438006

End: 2439241

Strand: Direct

Name: amiA [C]

Synonym: BALH_2274

Alternate gene names: 118477928

Gene position: 2438006-2439241 (Clockwise)

Preceding gene: 118477926

Following gene: 118477929

Centisome position: 46.38

GC content: 35.28

Gene sequence:

>1236_bases
TTGAAGAACAAATTAATTGCTACAGGAATTCTTGCTGGAAGTTTACTATCATATTCTACTAGTATTGTTGCAGATACTCA
CAGATTCCCAGATATTCCTGCATGGGCTGACAAATCCGTTACTTATTTAGTTGATAAACAAGTATTGAGTGGTTATCCAG
ATGGGACTTTTGGTTCAAGTGATACACTAGATAGAGCTTCTGCAGCAACAATTATGACAAAGGCTCTTGGTATACACATT
GATTTAAATGCAAAACCATCTTTTAAAGATTCACAAAACCACTGGGGAACCCCTTATATTGCCGCAGCTGAAAAGGCAGG
AATCATTAAAGGTGAAGGAAATGGAATATTTAATCCTTCTGGAAAAGTTACTCGTGTTGCTATGGCTACTATGCTAGTGA
ATGCATATAAACTACAAAATAAAAACACTAGCAATGGACAACGTAAATTTGAAGATTTAAAGGGCCATTGGGGTGAAAAG
TTCGCAAATACTTTAATTGATTTGAAAATTTCAGTTGGTACAGATAATGGCTGGCAACCAAATAGATTCATAACACGCGC
TGAAGCTGCACAACTAACTGCAAAAACAGATATGCTTCAATATAGTCATAGTAATCCTTTAGAAAATAAAACCATAATTA
TTGACCCCGGACATGGCGGCGAAGATCCTGGAAAAAACACAAAGGGATTACCTGAAAGTAAGATTGTACTAGATACTTCT
TTACGTCTTCAACAATTGCTTGAAAAACATACTCCATTTACAGTTTTACTAACTCGTGAATCTGATACTAGACCTGGACA
TGATCAAAAAAGCTCTTTACAGGAACGTGTTAAGTTTGCTAAGCAAAATCAGGGTGATATCTTTATAAGCATTCATGCAA
ATGCTTTTAACGGTAATGCAAAAGGGACAGAAACATATTACTATAAATCTTCTAAATCTGAAAAAACAAATCCTCATGTG
GAAGAAAGTCGTGTTTTAGCTGAAAAAATTCAAGAACGATTAGTAGACGCACTTCAAACACGTGATAGAGGTGTTAAACA
TGGAGATCTTCATGTTATAAGAGAAAATGACATGCCAGCTGTGTTAACGGAACTTGCTTTTATAGATAATGGTATTGATT
ACAGTAAGTTATCTACAGAAAATGGAAGACAGATTGCAGCAGAAGCCATTTACGAGGGGATTTTAGATTATTATGAATGG
AAGGGGAATAATGTATCTGAATATAGGTTGCAATAG

Upstream 100 bases:

>100_bases
CCAACTTAATCTATACTTTATTCGAAGTCATATGATTTAAAGATGAACTATAATTGATATAATTTACCAACTTGTAATAA
TTTCTTAGAGGTGCTAAATT

Downstream 100 bases:

>100_bases
GTAAATGAATATATAGTAATTCTCGAAATAAAGAATTCTATAATTTTTTTGCACTTTTTTAAACAATAATTTTAAATATG
TATTTAAAATTATTGTTTAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKNKLIATGILAGSLLSYSTSIVADTHRFPDIPAWADKSVTYLVDKQVLSGYPDGTFGSSDTLDRASAATIMTKALGIHI
DLNAKPSFKDSQNHWGTPYIAAAEKAGIIKGEGNGIFNPSGKVTRVAMATMLVNAYKLQNKNTSNGQRKFEDLKGHWGEK
FANTLIDLKISVGTDNGWQPNRFITRAEAAQLTAKTDMLQYSHSNPLENKTIIIDPGHGGEDPGKNTKGLPESKIVLDTS
LRLQQLLEKHTPFTVLLTRESDTRPGHDQKSSLQERVKFAKQNQGDIFISIHANAFNGNAKGTETYYYKSSKSEKTNPHV
EESRVLAEKIQERLVDALQTRDRGVKHGDLHVIRENDMPAVLTELAFIDNGIDYSKLSTENGRQIAAEAIYEGILDYYEW
KGNNVSEYRLQ

Sequences:

>Translated_411_residues
MKNKLIATGILAGSLLSYSTSIVADTHRFPDIPAWADKSVTYLVDKQVLSGYPDGTFGSSDTLDRASAATIMTKALGIHI
DLNAKPSFKDSQNHWGTPYIAAAEKAGIIKGEGNGIFNPSGKVTRVAMATMLVNAYKLQNKNTSNGQRKFEDLKGHWGEK
FANTLIDLKISVGTDNGWQPNRFITRAEAAQLTAKTDMLQYSHSNPLENKTIIIDPGHGGEDPGKNTKGLPESKIVLDTS
LRLQQLLEKHTPFTVLLTRESDTRPGHDQKSSLQERVKFAKQNQGDIFISIHANAFNGNAKGTETYYYKSSKSEKTNPHV
EESRVLAEKIQERLVDALQTRDRGVKHGDLHVIRENDMPAVLTELAFIDNGIDYSKLSTENGRQIAAEAIYEGILDYYEW
KGNNVSEYRLQ
>Mature_411_residues
MKNKLIATGILAGSLLSYSTSIVADTHRFPDIPAWADKSVTYLVDKQVLSGYPDGTFGSSDTLDRASAATIMTKALGIHI
DLNAKPSFKDSQNHWGTPYIAAAEKAGIIKGEGNGIFNPSGKVTRVAMATMLVNAYKLQNKNTSNGQRKFEDLKGHWGEK
FANTLIDLKISVGTDNGWQPNRFITRAEAAQLTAKTDMLQYSHSNPLENKTIIIDPGHGGEDPGKNTKGLPESKIVLDTS
LRLQQLLEKHTPFTVLLTRESDTRPGHDQKSSLQERVKFAKQNQGDIFISIHANAFNGNAKGTETYYYKSSKSEKTNPHV
EESRVLAEKIQERLVDALQTRDRGVKHGDLHVIRENDMPAVLTELAFIDNGIDYSKLSTENGRQIAAEAIYEGILDYYEW
KGNNVSEYRLQ

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall, S-layer (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=265, Percent_Identity=27.1698113207547, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR001119 [H]

Pfam domain/function: PF01520 Amidase_3; PF00395 SLH [H]

EC number: 3.5.1.28

Molecular weight: Translated: 45540; Mature: 45540

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLIATGILAGSLLSYSTSIVADTHRFPDIPAWADKSVTYLVDKQVLSGYPDGTFGSS
CCCCEEEHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCC
DTLDRASAATIMTKALGIHIDLNAKPSFKDSQNHWGTPYIAAAEKAGIIKGEGNGIFNPS
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCEEECCC
GKVTRVAMATMLVNAYKLQNKNTSNGQRKFEDLKGHWGEKFANTLIDLKISVGTDNGWQP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCEEEEEEEECCCCCCCC
NRFITRAEAAQLTAKTDMLQYSHSNPLENKTIIIDPGHGGEDPGKNTKGLPESKIVLDTS
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECH
LRLQQLLEKHTPFTVLLTRESDTRPGHDQKSSLQERVKFAKQNQGDIFISIHANAFNGNA
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCC
KGTETYYYKSSKSEKTNPHVEESRVLAEKIQERLVDALQTRDRGVKHGDLHVIRENDMPA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHH
VLTELAFIDNGIDYSKLSTENGRQIAAEAIYEGILDYYEWKGNNVSEYRLQ
HHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MKNKLIATGILAGSLLSYSTSIVADTHRFPDIPAWADKSVTYLVDKQVLSGYPDGTFGSS
CCCCEEEHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCC
DTLDRASAATIMTKALGIHIDLNAKPSFKDSQNHWGTPYIAAAEKAGIIKGEGNGIFNPS
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCEEECCC
GKVTRVAMATMLVNAYKLQNKNTSNGQRKFEDLKGHWGEKFANTLIDLKISVGTDNGWQP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCEEEEEEEECCCCCCCC
NRFITRAEAAQLTAKTDMLQYSHSNPLENKTIIIDPGHGGEDPGKNTKGLPESKIVLDTS
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECH
LRLQQLLEKHTPFTVLLTRESDTRPGHDQKSSLQERVKFAKQNQGDIFISIHANAFNGNA
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCC
KGTETYYYKSSKSEKTNPHVEESRVLAEKIQERLVDALQTRDRGVKHGDLHVIRENDMPA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHH
VLTELAFIDNGIDYSKLSTENGRQIAAEAIYEGILDYYEWKGNNVSEYRLQ
HHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10475962; 12004073 [H]