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The map label for this gene is prpD [H]

Identifier: 118477756

GI number: 118477756

Start: 2240338

End: 2241858

Strand: Direct

Name: prpD [H]

Synonym: BALH_2094

Alternate gene names: 118477756

Gene position: 2240338-2241858 (Clockwise)

Preceding gene: 118477755

Following gene: 118477757

Centisome position: 42.62

GC content: 36.88

Gene sequence:

>1521_bases
TTGTTAGCACCGCCCGCCAGATGGGTGTTTGACCGACACCCATCATTAATAATAACGAATTTTCGACAGAAAGGGAAGGA
TAGCATGATTAAAACAAATGAAATTAAACAAAAAGATGCAATATTAGAAGAGATTACGGATTATGTATTAAATAAAGAGG
TAACAAGTGCAGAAGCATTCAGTACTGCTCGTTACGTATTACTTGATACACTTGGATGCGGAATTTTAGCATTACAATAT
CCAGAGTGTACGAAATTATTAGGACCAGTTGTACCAGGAACAATCGTGCCAAATGGAACACGAGTGCCAGGTACGTCTTA
TGTATTAGATCCAGTGAAAGGTGCATTTAATATCGGATGTATGATCCGTTGGTTAGACTATAACGATACTTGGCTTGCAG
CAGAATGGGGACATCCATCTGATAACCTTGGCGGCATTTTAGCAGTTGCAGATTATATTAGCCGTGTTCGTATATCAGAA
GGAAAAGAACCGTTAAAAGTACGTGAAGTATTAGAAATGATGATTAAAGCACATGAAATTCAAGGTGTATTAGCTTTAGA
AAACAGCTTAAACCGGGTTGGTCTTGACCACGTATTATACGTAAAAGTAGCAACAACTGCTGTAGTTGCGAAAATGCTTG
GCGGAACACGTGAAGAAATCTTTAATGCATTATCACATGCATGGATTGATAATTCTAGTCTTCGTACATATCGTCACGCT
CCAAATACTGGATCACGTAAATCATGGGCAGCAGGTGATGCAACAAGTCGCGGTGTTCACCTTGCAATGACTGCTTTAAA
AGGTGAAATGGGTTATCCAACAGCATTATCTGCACCGGGTTGGGGATTCCAAGATGTATTATTTAATAAACAAGAATTAA
AGTTAGCTAGACCATTAGAGTCTTATGTAATGGAAAATGTATTATTTAAAGTTTCATATCCAGCAGAATTCCATGCACAA
ACAGCTGCAGAATGTGCTGTAAAATTACATCCGGAAATTAAAGAAAGATTAGATGAAATTGACCGTATTACAATTACAAC
TCATGAATCAGCAATTCGTATTATTGATAAAGAAGGTCCATTAAATAACCCAGCTGATCGTGATCATTGTTTACAATATA
TTACGGCAATTGGTTTATTAAAGGGAGATATCGTTGCGGATGATTATGAGGATGCAGTAGCAAATGATCCACGTGTAGAT
GAATTACGTAATAAGATGGTTGTTGTTGAAAACAAACAGTACAGTTTAGATTACCTTGACCCGAACAAGCGCTCAATCGC
CAACGCTGTTCAAGTTCATTTCAAGGATGGAACTGTAACAGAAAACGTGGAATGTGAATATCCACTTGGTCACCGTTTCC
GTAGAGACGAAGCAATTCCAAAAGTTGTTCAAAAATTCACTGCAAATATGGCAGGTCATTATTCTAGTAAACAGCAAGAA
CAAATTCATGAAGTTTGTTTAAATGAAGAGAAACTAGAAAATATGAATGTAAACGAATTTGTAGATCTATTCTTAATTTA
A

Upstream 100 bases:

>100_bases
CGCACGTTATTGAACAACATACGAACAATCGTTTGTTCCGTCCACGTGTAAATTATATTGGCGAGCGACATGTACTTAGT
AAGTAAAAACAACTGGGGAG

Downstream 100 bases:

>100_bases
TAATAGGGGGAATTTAGAATGGCTTGGGTTGTGAATAAACAGTCAACACAAGAGGAGCTTGCGAATCGCTTCCGAGCTTT
AGTAGAAGCAAATGAAATTT

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLAPPARWVFDRHPSLIITNFRQKGKDSMIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQY
PECTKLLGPVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISE
GKEPLKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHA
PNTGSRKSWAAGDATSRGVHLAMTALKGEMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQ
TAAECAVKLHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVD
ELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQE
QIHEVCLNEEKLENMNVNEFVDLFLI

Sequences:

>Translated_506_residues
MLAPPARWVFDRHPSLIITNFRQKGKDSMIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQY
PECTKLLGPVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISE
GKEPLKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHA
PNTGSRKSWAAGDATSRGVHLAMTALKGEMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQ
TAAECAVKLHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVD
ELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQE
QIHEVCLNEEKLENMNVNEFVDLFLI
>Mature_506_residues
MLAPPARWVFDRHPSLIITNFRQKGKDSMIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQY
PECTKLLGPVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISE
GKEPLKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHA
PNTGSRKSWAAGDATSRGVHLAMTALKGEMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQ
TAAECAVKLHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVD
ELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQE
QIHEVCLNEEKLENMNVNEFVDLFLI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=466, Percent_Identity=62.8755364806867, Blast_Score=630, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=52.880658436214, Blast_Score=522, Evalue=1e-149,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 56687; Mature: 56687

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAPPARWVFDRHPSLIITNFRQKGKDSMIKTNEIKQKDAILEEITDYVLNKEVTSAEAF
CCCCCCCHHHCCCCCEEEECCHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
STARYVLLDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGC
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
MIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEPLKVREVLEMMIKAHEI
EEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGVLALENSLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHA
CCHHHHHHCHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECC
PNTGSRKSWAAGDATSRGVHLAMTALKGEMGYPTALSAPGWGFQDVLFNKQELKLARPLE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
SYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTHESAIRIIDKEGP
HHHHHHHHEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCEEEEEECCCC
LNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLD
CCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHCCCCCHHHHCCCEEEEECCCEEEECCC
PNKRSIANAVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQE
CCHHHHHHEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QIHEVCLNEEKLENMNVNEFVDLFLI
HHHHHHCCHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLAPPARWVFDRHPSLIITNFRQKGKDSMIKTNEIKQKDAILEEITDYVLNKEVTSAEAF
CCCCCCCHHHCCCCCEEEECCHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
STARYVLLDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGC
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
MIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEPLKVREVLEMMIKAHEI
EEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGVLALENSLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHA
CCHHHHHHCHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECC
PNTGSRKSWAAGDATSRGVHLAMTALKGEMGYPTALSAPGWGFQDVLFNKQELKLARPLE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
SYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTHESAIRIIDKEGP
HHHHHHHHEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCEEEEEECCCC
LNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLD
CCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHCCCCCHHHHCCCEEEEECCCEEEECCC
PNKRSIANAVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQE
CCHHHHHHEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QIHEVCLNEEKLENMNVNEFVDLFLI
HHHHHHCCHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]