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The map label for this gene is trmA [C]

Identifier: 118476178

GI number: 118476178

Start: 485095

End: 486474

Strand: Direct

Name: trmA [C]

Synonym: BALH_0425

Alternate gene names: 118476178

Gene position: 485095-486474 (Clockwise)

Preceding gene: 118476177

Following gene: 118476179

Centisome position: 9.23

GC content: 37.32

Gene sequence:

>1380_bases
ATGATACAAAAGCAACATGAGAGTAAGTTGGAAGTTGGTCAAACATTTCCTGTGACAATTAAACGTCTTGGGATTAACGG
AGAAGGCGTTGGTTATTTTAAGAGACAAGTTGTTTTCATTCCAGGGGCATTACCAGGAGAAGAAGTTGTTGCAGAAACGA
CGAAAATTCAGCGTGGCTTCGCTGAAGCGAAAGTGAAAAAAGTTCGTAAAGCTTCACCGCATCGTGTGAAAGCACCGTGC
CCAGTATATGAGGAGTGTGGCGGTTGCCAATTGCAACATTTAGATTATAAAGAACAATTGAATCAAAAGCGTGATATCGT
TGTACAAGCATTCCAAAAGTATATGAACAACGGTTTGGAAGAGAAAATTCGTCCGACGCTTGGCATGGAAAACCCATGGC
ATTATCGTAATAAGAGTCAATTACAAGTAGGACGTAAAGATGAAAAGGTTATTACGGGGCTGTATAAACAAAATTCACAT
CAGTTAATTGATATTGCTCATTGTATGATTCAACATAAAGCAACGAATGAAGCGACAAAAGTTGTAAGACGTATTTTAGA
AAAATTAAATGTTTCTATTTACAATGAGAAAAAACAAAAAGGTTTAGTACGTACAATTGTGACACGTACTGCAGTGCAAA
CAGGGGAAGTACAAGTTACACTTATTACAACAAAAGAAGAGTTGCCAAATAAAGAACAATTTATTGCAGAAGTACAAAAA
CAGATGCCAGCTGTTAAATCAATTATGCAAAATGTAAACTGGCGTAAAACATCGGTTATTTTCGGTGATAAAACATTTAA
ATTAGCTGGAAAAGAAGTAATTCAAGAAACACTTGGTGATTTATCATTTGAATTATCAGCACGTGCATTCTTCCAGTTGA
ATCCAGAACAAACGGTTGTTTTATACAATGAAGCGAAAAAAGCCGCTGCTTTAACAGGCAATGAAAAAATTGTAGATGCA
TATTGTGGCGTTGGTACAATCGGTCTTTGGCTTGCAAATGATGCAGCGGAAGTACGTGGTATGGATGTAATTCCAGAAGC
GATTGCAGATGCAAGAAAAAATGCGAAGCGTCACGGATTTACAAATACGAAATATGAAGCAGGTAAAGCTGAACAATGGT
TACCTAAATGGGTAAAAGAAGGATGGCGTCCAGATGTAATTGTTGTCGATCCACCACGTACAGGTTGCGATGATAAATTA
CTGGAAACAATTTTAAAAGTGAAGCCGAAACAAGTTGTTTACGTATCTTGTAACCCTTCTTCCTTAGCACGTGATGTACA
AGCGTTAATGAAGAGTTATGAAGTGGAATATGTACAACCAGTTGATATGTTCCCGCACACAGCTCATGTAGAAAATGTAG
TGAAGCTTGTTAGAAAGTAA

Upstream 100 bases:

>100_bases
CCTGATGATGCATTTTTAGAAAAAGTGAAACAAGAGTGTGAACCATACTGCAGTTATGCAGCGTTATATTTATGGAAAAG
TATAGAGTAGAGGTGTAATA

Downstream 100 bases:

>100_bases
AGTTTATTCATATTCCCTCATGATATGAGGGAATATTTTCTGTGAAGGAGAATAAGATGAACAATTATAAATTAACAATT
CAGTACGACGGTGCACGTTT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFQKYMNNGLEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK

Sequences:

>Translated_459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFQKYMNNGLEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
>Mature_459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFQKYMNNGLEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI51173878, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI269784678, Length=383, Percent_Identity=25.8485639686684, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI269784680, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI269784676, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI20149688, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789148, Length=458, Percent_Identity=26.6375545851528, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=24.8041775456919, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1790403, Length=211, Percent_Identity=25.5924170616114, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=32.7272727272727, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=466, Percent_Identity=24.4635193133047, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24666579, Length=445, Percent_Identity=28.5393258426966, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51945; Mature: 51945

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGF
CCCCCCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
AEAKVKKVRKASPHRVKAPCPVYEECGGCQLQHLDYKEQLNQKRDIVVQAFQKYMNNGLE
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHCHHHHHHHHHHHHHHCCCHH
EKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSHQLIDIAHCMIQHKATNEATK
HHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
VVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHEECCCEEEEEEECHHHCCCHHHHHHHHHH
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEE
LYNEAKKAAALTGNEKIVDAYCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGF
EEECCCHHHHCCCCCHHHHHHCCCCHHHEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCC
TNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKLLETILKVKPKQVVYVSCNPS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHH
SLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
HHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGF
CCCCCCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
AEAKVKKVRKASPHRVKAPCPVYEECGGCQLQHLDYKEQLNQKRDIVVQAFQKYMNNGLE
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHCHHHHHHHHHHHHHHCCCHH
EKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSHQLIDIAHCMIQHKATNEATK
HHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
VVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHEECCCEEEEEEECHHHCCCHHHHHHHHHH
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEE
LYNEAKKAAALTGNEKIVDAYCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGF
EEECCCHHHHCCCCCHHHHHHCCCCHHHEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCC
TNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKLLETILKVKPKQVVYVSCNPS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHH
SLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
HHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]