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The map label for this gene is alkA [H]

Identifier: 118476177

GI number: 118476177

Start: 484209

End: 485084

Strand: Direct

Name: alkA [H]

Synonym: BALH_0424

Alternate gene names: 118476177

Gene position: 484209-485084 (Clockwise)

Preceding gene: 118476176

Following gene: 118476178

Centisome position: 9.21

GC content: 35.39

Gene sequence:

>876_bases
ATGGGGAAACGTATGTGGAGCGAACATGTTACGTTAGAGTATCCGTATTATTTTGAAGAAGTATTAAAACGTTTATCTTT
TGATCCTCTTAACGTCATTCAATTAGATGAGAAAGTTATTTATGTTCCGATTTGTATAGACGAGGAACAGATTGTTGTTC
GCCTGCAAGGGATTGGTACTGTTCAAAATCCACAATTTTGGATTTCTAGTCAGACAGGAGATCCAGAGAAAGTCATGAAA
CGAATGAGGGCCATTTTTCATTGGAATGAACCGTTTCAAGATATACAAAATCATTTTTTAAACACATCATTACGTCCACT
ATTTGAAACATATGCTTATACTCCAATTATTTTAGAATTTGATTATTTTGCTTGCCTTCTTCGCTGTATCATTCATCAAC
AAATAAATTTGAAATTTGCTACTGTATTAACAGAACAGTTTGTGAAACGATATGGAACAGAAAAGAACGGCGTATTCTTT
TTCCCAACTCCAGAAAGAGTAGCAAACATTTCAATAGAGGAATTGAGAGAGCAGAAGTTTAGTCAGCGAAAAGCTGAATA
TATAGTAGGATTAGGTCAAAGTATTGCCAGCAGTACATTAAATTTAGCGAGTATAGAAACCAGGGCGGAAGAAGAGGTTT
CAGCACAATTGTTACCAATTAGGGGGATTGGTACATGGACAGTGCAAAATTTTTTAATGTTTGGGCTTGGACGGAAAAAT
ATGTTTCCGAAAGCGGACATTGGGATTCAGCGTGCAGTACAAGGTTTATTTCAATTAGATGATAAACCTGATGATGCATT
TTTAGAAAAAGTGAAACAAGAGTGTGAACCATACTGCAGTTATGCAGCGTTATATTTATGGAAAAGTATAGAGTAG

Upstream 100 bases:

>100_bases
AAGGGTATCATTTTAAAAGTCTAGATGACTTAGTAAAAAGCAATCAACCGTAAGCATGTATGCTTGCGGTTTTCTCATGC
TATAATGATGTGTAAAAAGG

Downstream 100 bases:

>100_bases
AGGTGTAATAATGATACAAAAGCAACATGAGAGTAAGTTGGAAGTTGGTCAAACATTTCCTGTGACAATTAAACGTCTTG
GGATTAACGGAGAAGGCGTT

Product: DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGKRMWSEHVTLEYPYYFEEVLKRLSFDPLNVIQLDEKVIYVPICIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMK
RMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFF
FPTPERVANISIEELREQKFSQRKAEYIVGLGQSIASSTLNLASIETRAEEEVSAQLLPIRGIGTWTVQNFLMFGLGRKN
MFPKADIGIQRAVQGLFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE

Sequences:

>Translated_291_residues
MGKRMWSEHVTLEYPYYFEEVLKRLSFDPLNVIQLDEKVIYVPICIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMK
RMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFF
FPTPERVANISIEELREQKFSQRKAEYIVGLGQSIASSTLNLASIETRAEEEVSAQLLPIRGIGTWTVQNFLMFGLGRKN
MFPKADIGIQRAVQGLFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
>Mature_290_residues
GKRMWSEHVTLEYPYYFEEVLKRLSFDPLNVIQLDEKVIYVPICIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMKR
MRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFF
PTPERVANISIEELREQKFSQRKAEYIVGLGQSIASSTLNLASIETRAEEEVSAQLLPIRGIGTWTVQNFLMFGLGRKNM
FPKADIGIQRAVQGLFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 33952; Mature: 33820

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRMWSEHVTLEYPYYFEEVLKRLSFDPLNVIQLDEKVIYVPICIDEEQIVVRLQGIGT
CCCCCCCCCEEEECCHHHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCEEEEEEECCCC
VQNPQFWISSQTGDPEKVMKRMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEF
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEH
DYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTPERVANISIEELREQKF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHHHHH
SQRKAEYIVGLGQSIASSTLNLASIETRAEEEVSAQLLPIRGIGTWTVQNFLMFGLGRKN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCC
MFPKADIGIQRAVQGLFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKRMWSEHVTLEYPYYFEEVLKRLSFDPLNVIQLDEKVIYVPICIDEEQIVVRLQGIGT
CCCCCCCCEEEECCHHHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCEEEEEEECCCC
VQNPQFWISSQTGDPEKVMKRMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEF
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEH
DYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTPERVANISIEELREQKF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHHHHH
SQRKAEYIVGLGQSIASSTLNLASIETRAEEEVSAQLLPIRGIGTWTVQNFLMFGLGRKN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCC
MFPKADIGIQRAVQGLFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]