Click here to switch to the map view.

The map label for this gene is dus2 [H]

Identifier: 118476082

GI number: 118476082

Start: 374734

End: 375744

Strand: Direct

Name: dus2 [H]

Synonym: BALH_0325

Alternate gene names: 118476082

Gene position: 374734-375744 (Clockwise)

Preceding gene: 118476081

Following gene: 118476083

Centisome position: 7.13

GC content: 41.35

Gene sequence:

>1011_bases
GTGAGAGGATTTGAATTTGAAATGATAGATAATTTTTGGCGTGATTTACCACGACCATTTTTTGTACTTGCACCAATGGA
AGATGTGACAGACGTTGTTTTTCGTCACGTAGTAAGTGAGGCTGGCCGTCCAGATGTATTCTTCACAGAGTTCACAAATT
CGGATAGCTATTGTCATCCAGAAGGTATGAAAAGCGTACGTGGCCGTTTAATTTTTACAGAAGACGAACAGCCAATGGTG
GCACATATTTGGGGAGATAACCCTGAATATTTCCGTCAAATGAGTATCGGCATGGCAGAACTAGGATTTAAAGGTATCGA
TATTAATATGGGCTGCCCTGTACCGAACGTAGCATCAAGAGGAAAAGGTAGTGGTCTTATTCTACGTCCAGATGTTGCGG
CAGAACTTATTCAAGCTGCAAAAGCAGGCGGACTACCTGTCAGCGTAAAAACACGCCTTGGCTTTAAAGAGTTAAGCGAA
TGGGAAGATTGGTTAACGCACATTTTCAAACAAGATATTGCGAACCTTTCTATTCATTTACGCACAAGAGAAGAAATGAG
CCAAGTAGATGCGCACTGGGAGCTAATTCCGGAGATTAAAAAATTACGTGATCGCATTGCACCAAATACGCTCCTAACAA
TCAACGGAGACATCCTTGATCGTAAAATGGGACTGGAACTTGCTGAAAAATATGGTATTGATGGCGTAATGATCGGACGA
GGCATCTTTAAAAATCCATTTGCGTTTGAAAAAGAGCCAAGAGAGCATAGCAGTAAAGAGCATCTAGATCTTTTAAGACT
ACAACTTGATCTTCAAGATCAATATGCAGAAGTACTGCCACGATCCATAACAGGATTACATCGCTTCTTCAAAATTTATG
TAAAAGGATTCCCTGGGGCTGCTGAACTACGAAATCAATTGATGAGCACGAAATCGACTGATGAAGTGCGTGGGTTGCTT
GATAAGTTTGAGGCGAGTGTTAATGTGGTAGAAGGTAGTGAGACAGTGTAA

Upstream 100 bases:

>100_bases
TGTAGCTTGGCTTAAGTTGGTATAATAAAAAAGCAGTTGATATAGGAAAATCTATACCATCTGCTTTTTGGTTTATTGAG
AGTTTGAAATTGTATTCATA

Downstream 100 bases:

>100_bases
CTGTTGAAAGTTAGAGGGGCTGGAGTGTAGTAATTTTATTAGTGTAAGGTGAACCGTATCATAAGTAAGCGAGGTATTCT
ATATAAAACAAGGGTAAGAA

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRGFEFEMIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMV
AHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSE
WEDWLTHIFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGR
GIFKNPFAFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRGLL
DKFEASVNVVEGSETV

Sequences:

>Translated_336_residues
MRGFEFEMIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMV
AHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSE
WEDWLTHIFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGR
GIFKNPFAFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRGLL
DKFEASVNVVEGSETV
>Mature_336_residues
MRGFEFEMIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMV
AHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSE
WEDWLTHIFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGR
GIFKNPFAFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRGLL
DKFEASVNVVEGSETV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=322, Percent_Identity=24.8447204968944, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI239788483, Length=174, Percent_Identity=32.183908045977, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI239788462, Length=174, Percent_Identity=32.183908045977, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=28.75, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=28.9915966386555, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI145693211, Length=339, Percent_Identity=25.3687315634218, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=26.7605633802817, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=312, Percent_Identity=26.2820512820513, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585320, Length=265, Percent_Identity=26.4150943396226, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38228; Mature: 38228

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFEFEMIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHP
CCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
EGMKSVRGRLIFTEDEQPMVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASR
HHHHHHCCEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHCC
GKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHL
CCCCCEEECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEE
RTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGR
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECC
GIFKNPFAFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGA
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AELRNQLMSTKSTDEVRGLLDKFEASVNVVEGSETV
HHHHHHHHCCCCHHHHHHHHHHHHHCEEEECCCCCC
>Mature Secondary Structure
MRGFEFEMIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHP
CCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
EGMKSVRGRLIFTEDEQPMVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASR
HHHHHHCCEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHCC
GKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHL
CCCCCEEECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEE
RTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDILDRKMGLELAEKYGIDGVMIGR
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECC
GIFKNPFAFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGA
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AELRNQLMSTKSTDEVRGLLDKFEASVNVVEGSETV
HHHHHHHHCCCCHHHHHHHHHHHHHCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]