Click here to switch to the map view.

The map label for this gene is kinE [H]

Identifier: 118476083

GI number: 118476083

Start: 376046

End: 378079

Strand: Direct

Name: kinE [H]

Synonym: BALH_0326

Alternate gene names: 118476083

Gene position: 376046-378079 (Clockwise)

Preceding gene: 118476082

Following gene: 118476084

Centisome position: 7.15

GC content: 35.4

Gene sequence:

>2034_bases
TTGGAGGCTATGCAAATGTTAAGTGATCAAACGAGATATTCTAGGCTCGCGAATATAACCAAAATAATAAATACAAAATT
AGAACTACGTGAAGTATTGCAGCGTGTAACAATGGCGATATCAGAGGAGATTGTTAGGTGTGACGCTGTTGGAATTTATT
TACCTGAAGAAGATGGAACATTTAGAGGGTTTGCAGGAAAACCAGAGACCATAAATGGCGTAACGCTCGATACGCAGGTA
ATTGACCCTGAAGTAGACTTACTGGCAAAAGAAGTTATTGAAACTAAAAAAACCATCTATATTCCTGATACCTCAAAGGA
TCATCGACCGGATCCGAGACCAGTCGATGCGTTCAAAATTAAGTCTTTATTAGCTCTGCCTATCTCATTTGGGCAAGAGT
TATTTGGTCTTGTTTTTTTATTTGATTATGGAGTTCCAATGAACTTAACAGGTTCAGAAATTCAAAGTGTTGAAGCTTAT
GTAAACATGGCGGCGGTCGCCATTCAAAACGCAAAAAATTTAACCCAAAAAGAAAATCTTATTGCCGAGAAGCAGCTGTT
ACTAAATGTTACCCGTGATTTATCAATGTGTTCCTCAATACAGGAGAGTTTTGAAAAATGCTTTTTTTACTTAGGACAGA
TTTTAGAGAGTAAAAACATGGCTGCCCACCTTTTAGATCCGCTAGACAAAATAACGATTAAGACAACGGAGTTAAGCTCG
GGCTGTGATTGGATAGAAGCAGATTGGATGGAGAAAAGCTATGAAGCCAAGATTCAAGAGATTATTCGAACAAAACATAT
AGATAGTAAGGGTTTACTGATGATTCCATTGGTTTCAATGGGAGAAGTATTAGGGGTAATCGTCGTTGGCAAAGAGGAAA
AAACTCATAATTACGATGATTCCCGAATTCAACTGGCAAAATCTATCGTTGATGCTACAGCCCCTACATTTTCAAACTTG
TTATATATGGATCAACTTGAAAGCATGGTGGAAGAACGAACGAGAGAACTAGCTGCTGCTAATGAAAAAGTGACAAGTGT
GATTGAAAGTATTACGGATGGATTCTTGACTTTGAATAATAAATGGGAATTTACGTATGTAAATAAACATCAACATTTCC
CACAAAGAAAAACAGCAAAAGACGTATTAGGTAAGAATATATGGGAGGTCTTCCCAAGTAGCGTAGATACAGTTATATAT
AAGGAGCTTCATCGTGTAATGTCAGAGCGAACTACAGTTCATTTTGAATTTTTTTCTACATTTGATGAATATTGGCATGA
AGTTATTGCATATCCGTATGATGATGGTATTTGTTGTATTTGTAAAAACATAACAGAAAAAAAGCAATATGAGCAGGAAT
TGAAAAGGTTATCCAACATAGAGTTAATAGGACAAATGGCAGCAGGTATCAGCCACGAGATTAGAAATCCAATGACAACA
ATACGAGGATTTTTGCAGTTATTAAAAGAAGAGAATAACTATGAGAAACATAATAACTACTTTAATTTAATGGTTGAAGA
ACTTGACCGTGCCAATTCTATTATTACTGAATTTCTCTCAATAGGTAATACAAAGAAATCAGATTTGCAGATGTTAGATT
TAAACTCAATTATCCACGATATTATTCCTTTAATAAAGATAGATACGCATAATCAAAATAAATATATTCAAGTCGATACA
AATGACGTTCCAGCATTACTTTTAAATCGTAATGAGATAAGGCAATTATTAATGAACCTATACCGTAATGGCTTAGAAGC
GATGAGTACAGGGAAAACTCTAACCATTAGCACCTATAAGGAAGGGAAAAATTGTGTGGTGCTTGCAGTGCGGGATGAAG
GGAAAGGTATTAGCCCTGAAGTATTAGAGAAACTGGGGACTCCATTTTACACAACCAAAGAGAATGGAACTGGATTGGGA
TTAGGCGTATGTTATGCCATTGCTACCCGTCATAATGCAAAAATAGAAATTCAAACAGGATCGGAAGGCACTACCTTTTT
TGTTAAATTTAATTATGAAAATAATAAAAAATAA

Upstream 100 bases:

>100_bases
TTCTGCTAGTGCAAAGAGGTTAGGTTGGATTCAGGCTGATAATAAGTGGGGGGATGAATCACCCCTCACTTATTAAAGTT
GCACTTTATTACGCCTATAA

Downstream 100 bases:

>100_bases
TCTTATTTAGATTAGTTACGGTAAACTGTATTACAAATAAAAAGTTAAAAATAATTAACATTTCAGGAGGAAACCCAATG
GCAATGAGCAACAACGATAT

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MEAMQMLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQV
IDPEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAY
VNMAAVAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFEKCFFYLGQILESKNMAAHLLDPLDKITIKTTELSS
GCDWIEADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNL
LYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIY
KELHRVMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTT
IRGFLQLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVDT
NDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGKNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLG
LGVCYAIATRHNAKIEIQTGSEGTTFFVKFNYENNKK

Sequences:

>Translated_677_residues
MEAMQMLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQV
IDPEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAY
VNMAAVAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFEKCFFYLGQILESKNMAAHLLDPLDKITIKTTELSS
GCDWIEADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNL
LYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIY
KELHRVMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTT
IRGFLQLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVDT
NDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGKNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLG
LGVCYAIATRHNAKIEIQTGSEGTTFFVKFNYENNKK
>Mature_677_residues
MEAMQMLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGTFRGFAGKPETINGVTLDTQV
IDPEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKIKSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAY
VNMAAVAIQNAKNLTQKENLIAEKQLLLNVTRDLSMCSSIQESFEKCFFYLGQILESKNMAAHLLDPLDKITIKTTELSS
GCDWIEADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDDSRIQLAKSIVDATAPTFSNL
LYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNNKWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIY
KELHRVMSERTTVHFEFFSTFDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTT
IRGFLQLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHDIIPLIKIDTHNQNKYIQVDT
NDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYKEGKNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLG
LGVCYAIATRHNAKIEIQTGSEGTTFFVKFNYENNKK

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=30.5676855895196, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=26.530612244898, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=26.6094420600858, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI48994928, Length=359, Percent_Identity=20.891364902507, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786600, Length=252, Percent_Identity=20.6349206349206, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=214, Percent_Identity=25.7009345794392, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788393, Length=201, Percent_Identity=26.865671641791, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 77044; Mature: 77044

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAMQMLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCC
FRGFAGKPETINGVTLDTQVIDPEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKI
CCCCCCCCCCCCCEEEECEEECCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHH
KSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAAVAIQNAKNLTQKENL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEKQLLLNVTRDLSMCSSIQESFEKCFFYLGQILESKNMAAHLLDPLDKITIKTTELSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEEEECCC
GCDWIEADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCCCH
SRIQLAKSIVDATAPTFSNLLYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
KWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHRVMSERTTVHFEFFST
EEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEHHHHH
FDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTT
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IRGFLQLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHD
HHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IIPLIKIDTHNQNKYIQVDTNDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYK
HHHHEEEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHCCHHHHHCCCEEEEEEEC
EGKNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCYAIATRHNAKIEIQTG
CCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEC
SEGTTFFVKFNYENNKK
CCCCEEEEEEECCCCCC
>Mature Secondary Structure
MEAMQMLSDQTRYSRLANITKIINTKLELREVLQRVTMAISEEIVRCDAVGIYLPEEDGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCC
FRGFAGKPETINGVTLDTQVIDPEVDLLAKEVIETKKTIYIPDTSKDHRPDPRPVDAFKI
CCCCCCCCCCCCCEEEECEEECCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHH
KSLLALPISFGQELFGLVFLFDYGVPMNLTGSEIQSVEAYVNMAAVAIQNAKNLTQKENL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEKQLLLNVTRDLSMCSSIQESFEKCFFYLGQILESKNMAAHLLDPLDKITIKTTELSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEEEECCC
GCDWIEADWMEKSYEAKIQEIIRTKHIDSKGLLMIPLVSMGEVLGVIVVGKEEKTHNYDD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCCCH
SRIQLAKSIVDATAPTFSNLLYMDQLESMVEERTRELAAANEKVTSVIESITDGFLTLNN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
KWEFTYVNKHQHFPQRKTAKDVLGKNIWEVFPSSVDTVIYKELHRVMSERTTVHFEFFST
EEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEHHHHH
FDEYWHEVIAYPYDDGICCICKNITEKKQYEQELKRLSNIELIGQMAAGISHEIRNPMTT
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IRGFLQLLKEENNYEKHNNYFNLMVEELDRANSIITEFLSIGNTKKSDLQMLDLNSIIHD
HHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IIPLIKIDTHNQNKYIQVDTNDVPALLLNRNEIRQLLMNLYRNGLEAMSTGKTLTISTYK
HHHHEEEECCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHCCHHHHHCCCEEEEEEEC
EGKNCVVLAVRDEGKGISPEVLEKLGTPFYTTKENGTGLGLGVCYAIATRHNAKIEIQTG
CCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEC
SEGTTFFVKFNYENNKK
CCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]