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The map label for this gene is oppA [H]

Identifier: 118475982

GI number: 118475982

Start: 229230

End: 230966

Strand: Direct

Name: oppA [H]

Synonym: BALH_0218

Alternate gene names: 118475982

Gene position: 229230-230966 (Clockwise)

Preceding gene: 118475981

Following gene: 118475983

Centisome position: 4.36

GC content: 34.83

Gene sequence:

>1737_bases
GTGGGGACAATGAAGAAAAAGACAAAAAAATGGGCTGGTGTATTTTCGGTATTACTGGGTAGTTCGCTTGTGTTATCTGC
TTGTGGGGGACAGGAGGATACGGCTTCTACAGAATCGGTAAAAAAGCAAGACTTAAAAGACGCTAAGATCGAATCAATTC
CAGCTACAGATAAAAAGAAAAGTCCAGAGAAAGCAAATCAGCGAAAGGATACTTTTATTACGGCCATTTCTAAGCCGGGG
GGAGTTTTCCTTCCATATTTCCAAGAGAATGGTTGGGATGGAAATGTAACGTCTGTAATTTTTGCGTCACTAGTAACAAC
AGACAAACAAAGTAAACCTATTCCGGATCTTGCAGAAAAATGGGATATTTCTGCTGACCAGTTAACATATACATTCCACT
TACGTAAAAATTTAAAATTTAGTGATGGATCGCCTTTAACTGCAGATGACGTAGCATTTACGTTAACACTACTTCATGAT
AAAGCGTATGAAGGTGGATTGGATATTGCTCAATATGCTGTTAAAGGCGGGAAAGAATATAAAGAAGGAAAAACAACTTC
TATTGAAGGGATTCAAGTTGTTGATCCACAGACAATTAAAATTACAACTGAAAAAGTTAATTCACAGGCGCTAACTAATT
TAGGTGGCCCAGTGCTATCAAAAGCTTATTATGGAAAAGATTATAAACAAAATACAAGTTTGGACTATTTAAAAGCATTA
TATGGGCAACCAATTGCAGCGGGTCCATATAAATTTGAAAAATATGTTCCAGGTCAAGAAGTACGCTTTGTTGCTAACGA
ACATTATTATGCAGGTAAACCAAAAATCAAAAACTTTATTTATAAAATCACATCAGGTGATACTGGATTCCAATTATTCC
AAACAGGTGAACTGGACTATAGTGGATTTAGAGCGAACCCTGAAAATATAGACCAATTAAAAGGATTAGAGTTTGCAAAT
ATTAATCTTGAATCATCAAGTGATATTGCTTATATCTATGTAAATAATAAGAAGTCGTATTTGAAAGATAAAAAGGTACG
CCAAGCACTTACTTATGGATTAGACCGTAAGAAGTATGTGGATACAGCTTTACAAGGATATGGCTCTGTTGCTAATGTAC
CAATTGCTCCAGTTTCATGGGCATATACAGAAGAAGGTATTAATAAATATCCACACGACGTAGAAAAGGCTAAAAAATTA
TTGGATGAAGCTGGTTGGAAAGTAGGTTCTGACGGGGTTCGTGAAAAAGATGGTCAAAAGTTAAAATTAACATACTATGC
ATCAAATACAGGTAAGACAAATGATATCTTTATTCCAATTGCAAAAGAAAGTTACAAAGAAATTGGTGTTGAATTAAATC
CAGAGTTGATGGACTTTAATACGATGCTTTCAAAAGTAGGAAAAGGTGACTACGATTTAGCGGCAGTTTCAACACCAGGG
ATTAGTGATCCAAGTGAAGTTGTAAGTGAGTACTTATCAACTAATCCAAAAAGTGATACGGGTTATAATAATCCGAAAGT
AGATGAGTTGATTGTAAAAGGTATAGGAACGACAGATATTGAAAAACGTAAAGCGATTTATAAAGAGCTGTATAAAGAGT
TAAGTGATGATCCACCAGTTATTTTATTAAACTATCGTAAACTACTTTATGCTCATAATGCACGTATAAAAGGAATTGAT
CCAGAAAAGTACGATAGCATCAGTTCCAACTTACCAGTGTTATCTATTGAACAATAA

Upstream 100 bases:

>100_bases
TTTTATGATTAAAACCGACTTTTCCGATTGGCTATAAACATGCTGTGGGAGGCATACATAAGCTAATAAGTTCAAAGGGT
ATCACTATTAAAAAGTTAGG

Downstream 100 bases:

>100_bases
GGGTTGACCTAAATAATAGAAGACGTTTTTTCACACAGCAATGAAATGGTAGCTGTTTTGAAAAAATGTTCTTCTATTTT
TAAGTAAATAGGATTGGGGA

Product: oligopeptide transporter, periplasmic-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MGTMKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTESVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPG
GVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHD
KAYEGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKAL
YGQPIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFAN
INLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKL
LDEAGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPG
ISDPSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGID
PEKYDSISSNLPVLSIEQ

Sequences:

>Translated_578_residues
MGTMKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTESVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPG
GVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHD
KAYEGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKAL
YGQPIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFAN
INLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKL
LDEAGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPG
ISDPSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGID
PEKYDSISSNLPVLSIEQ
>Mature_577_residues
GTMKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTESVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGG
VFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDK
AYEGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALY
GQPIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANI
NLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLL
DEAGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGI
SDPSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDP
EKYDSISSNLPVLSIEQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=27.4468085106383, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=27.2527472527473, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1789887, Length=502, Percent_Identity=24.1035856573705, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=26.1569416498994, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787495, Length=485, Percent_Identity=24.3298969072165, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787551, Length=524, Percent_Identity=22.5190839694656, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081878, Length=475, Percent_Identity=24.2105263157895, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789397, Length=464, Percent_Identity=22.198275862069, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64083; Mature: 63952

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTMKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTESVKKQDLKDAKIESIPATDKKK
CCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHCHHCCCCCCCCCC
SPEKANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEK
CCHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
WDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEY
CCCCHHHEEEEEEEECCCCCCCCCCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHH
KEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKAL
CCCCCCCCCCEEEECCCEEEEEHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHH
YGQPIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDY
HCCCCCCCCCCHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCC
SGFRANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYV
CCCCCCHHHHHHHCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHH
DTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQK
HHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCE
LKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPG
EEEEEEECCCCCCCEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
ISDPSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPV
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCE
ILLNYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ
EEECCHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
GTMKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTESVKKQDLKDAKIESIPATDKKK
CCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHCHHCCCCCCCCCC
SPEKANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEK
CCHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
WDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEY
CCCCHHHEEEEEEEECCCCCCCCCCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHH
KEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKAL
CCCCCCCCCCEEEECCCEEEEEHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHH
YGQPIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDY
HCCCCCCCCCCHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCC
SGFRANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYV
CCCCCCHHHHHHHCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHH
DTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQK
HHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCE
LKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPG
EEEEEEECCCCCCCEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
ISDPSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPV
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCE
ILLNYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ
EEECCHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]