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The map label for this gene is rlmI [H]

Identifier: 117919993

GI number: 117919993

Start: 1802402

End: 1803313

Strand: Direct

Name: rlmI [H]

Synonym: Shewana3_1545

Alternate gene names: 117919993

Gene position: 1802402-1803313 (Clockwise)

Preceding gene: 117919992

Following gene: 117919995

Centisome position: 36.25

GC content: 51.54

Gene sequence:

>912_bases
ATGCAACGTTTTATAGATGCACTGGCGACGATAGAAGTCGGTGAAGATGCCACGCGATTATTCCATGGCCGCGGTGGTTA
TTACCCTGGCTGCGAACATCTTTGTTTAGATTGGTTCGCGCCAGTATTGTTATTGACCAGTTTTATTCCCCTCGATGCGG
CTGAGTTGGCCGAGTGCCAACAGGCGATTACGGCGCGCTGGCAAGCGATACAGCCCAATCGGTTGTTAAACTTGGTGTAT
CAATACCGCAGTGCAGGCGAAACCTTTAGCCAAGTACTGGCGGGTGAGGTGCCCGATCCCCATGTGGTCACCGAGAACGG
CGCCCAGTTTCAGGTGCATTTATTGCGGGGACAAAACCACGGTTTGTTCCTTGATATGGCCAATGGCCGTGAATGGGTCA
GAGCCCATGCTCGCCATAAGAAAGTGCTGAATTTGTTTGCTTATACCTGTGGTTTCTCGGTGGCAGCACTGCAGGGCGGC
GCCGATGAAGTCGTGAACATGGACATGAGTAAAGGCGCGTTAGCCATAGGCAAGCAGAATCATCTTCTCAATGGCTTTAG
CGCGGGCGCACGTTTCTTAGGGCACGATATTTTTAAGTCATGGGGTAAGTTACGCAAGTTAGGCCCCTATGACATAGTTG
TTGCCGATCCGCCGAGTAATCAAAAGGGCAGTTTCGTGGCGACTAAGGATTACGTGCGTTTGATCAGGCATTTACCCGAG
TTGTTAGCCGAGAATGCCGAGGTGCTACTCTGCTTAAATGCACCGGAACTCGATACTCAATTTTTGCGCCAGCAGGTCGA
AGACGCAGCGCCCGCGCTCGAATTCCTTGAGCGCTTACCCAATCCCGCCGCATTTGTTGATGTTTCTGAGGAGAAATCAT
TAAAGGTGCTGCGCTACCGCTTAAAGGCGTAA

Upstream 100 bases:

>100_bases
ATCCGACGATGTGGATGATAAACTGGTGAGCCAATCGACAAGCACCGCTCCGATTTCTGAACCTGTCGATACCCATTCAA
CAGATAATAAGTAGTAGATA

Downstream 100 bases:

>100_bases
GCCCTCTGGTCTCATTAAACGCTGTCATACAACGCCGTCATGAAATGCCATCATAAGCCCTTGAGAGGCTTTATGATGGC
GTCGAATGATGTCGCTTCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLDAAELAECQQAITARWQAIQPNRLLNLVY
QYRSAGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAAPALEFLERLPNPAAFVDVSEEKSLKVLRYRLKA

Sequences:

>Translated_303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLDAAELAECQQAITARWQAIQPNRLLNLVY
QYRSAGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAAPALEFLERLPNPAAFVDVSEEKSLKVLRYRLKA
>Mature_303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLDAAELAECQQAITARWQAIQPNRLLNLVY
QYRSAGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAAPALEFLERLPNPAAFVDVSEEKSLKVLRYRLKA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=297, Percent_Identity=29.2929292929293, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787181, Length=233, Percent_Identity=29.1845493562232, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 33707; Mature: 33707

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLDAAELAECQ
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAITARWQAIQPNRLLNLVYQYRSAGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCC
GLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCEEEECCCC
HLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENAEVLLCLNAPELDTQFLRQQVEDAAPALEFLERLPNPAAFVDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
LKA
HCC
>Mature Secondary Structure
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLDAAELAECQ
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAITARWQAIQPNRLLNLVYQYRSAGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCC
GLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCEEEECCCC
HLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENAEVLLCLNAPELDTQFLRQQVEDAAPALEFLERLPNPAAFVDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
LKA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA