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The map label for this gene is purF [H]

Identifier: 117919931

GI number: 117919931

Start: 1725902

End: 1727416

Strand: Direct

Name: purF [H]

Synonym: Shewana3_1483

Alternate gene names: 117919931

Gene position: 1725902-1727416 (Clockwise)

Preceding gene: 117919930

Following gene: 117919934

Centisome position: 34.71

GC content: 48.71

Gene sequence:

>1515_bases
ATGTGTGGTATCGTCGGAATAGTTGGCCAATCATCGGTTAATCAGACCATTTATGACGCACTGACCGTGCTTCAACACAG
AGGTCAGGATGCGGCAGGTATTGTGACCGTTGATCGTGGTGCTTTCAGACTACGTAAAGCCAATGGTCTGGTCAAAGATG
TATTTGAAGTCAAACACATGCAACGCCTACAGGGCAATGCAGGTATTGGCCACGTACGTTATCCTACTGCAGGCAGTTCC
AGCGCGTCAGAAGCGCAGCCTTTCTATGTTAACTCGCCATTTGGGATTTCATTAGCCCATAACGGTAACTTAACCAACAC
GGTTGAATTAGCTGAAGGCCTGATCAAAAAACGTCGCCATGTGAACACCACATCCGACTCAGAAGTATTATTAAACCTGC
TGGCCGATGAGCTGCAAAAAACCACTAGCTTAATGCTGACGCCTGACGAAGTGTTCGACACTATCGCCAAAGTGCACGAG
CAAGCCCGTGGCGCCTATGCGGTTGTGGCCATGATTATCGGTCAAGGTCTGGTGGCATTTCGCGATCCATTCGGTATTCG
TCCACTGGTATTAGGTAAGCACGAAACCCCAACCGGTACTGAGTACATGGTGGCCTCTGAGAGCGTGGCCCTCGATGCCG
TGGGTTTTGAAGTGATGCGTGATGTGGCGCCAGGCGAAGCGATTTATATCTCTCTCGATGGCCAGCTTTACACTCGCCAA
TGCGCGAAAGAGCCAAGCTACTCACCTTGTATCTTCGAATTCGTGTATTTTGCCCGTCCAGATTCGACTATCGACAACGT
CTCTGTTTACGCCAGCCGCGTAAACATGGGGGCTAAGCTCGGTGAAAAGATCAAAAAAGAATGGTACGACCATGATATCG
ACGTGGTGATCCCTATTCCTGAAACCTCATGCGATATCGCATTAGAAATCGCCCGTTGCATGGACTTACCTTACCGCCAA
GGTTTTGTGAAGAACCGTTATATCGGCCGCACCTTTATTATGCCTGGTCAACAGGAGCGTAAAAAATCGGTACGCCGTAA
ACTCAATGCCATCAACACTGAGTTTAAAGGTAAGAACGTTTTGTTAGTCGATGACTCTATCGTGCGTGGTACCACGTCGG
AGCAAATCATTGAGATGGCGCGTGAAGCGGGTGCTAAGAAAGTGTACTTTGCCTCGGCGGCACCGGAAATCCGTTTCCCG
AACGTTTATGGTATCGATATGCCAACCTCGAATGAGCTGATTGCCCACGGTCGTGATGCCGATGAAATTGCTAAGCTGAT
TGGCGCCGATGGCATTATCTTCCAAAATCTGCCGGATCTGGTTGAAGCGGTGAGAATGGAAAACCCAGAGATCAAACGTT
TCGAAACCTCAGTGTTCGACGGTCACTACATCACTAACGATGTTGACCAATCTTACCTCGACCACTTAACTCAGTTACGT
AACGACGATGCCAAGGCCGACCGTAATAAAGACATTGGCACTAACTTAGAGTTACACAACGTTTGCCATCCATAA

Upstream 100 bases:

>100_bases
GATGAAGCACTTAAGCCGTATGGCTGGGGATTTACTTCGTCTTTTACTTATATTTCAACATGAAAGGGCATAGAACATCT
TACAATGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
GACGAAATTCTTAAGATGAAATTCTTAAGATAAAAGAGTGTGCTTGGCGTTAAGTGGGCACACTTTAAAATGCACAATAA
AAAAGCCAGTGATAAGTCAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHE
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHE
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP
>Mature_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHE
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.2105263157895, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.3598409542744, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=31.7919075144509, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=462, Percent_Identity=32.2510822510823, Blast_Score=228, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=510, Percent_Identity=49.8039215686275, Blast_Score=489, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=34.6076458752515, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=35.3065539112051, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI28573187, Length=479, Percent_Identity=33.8204592901879, Blast_Score=254, Evalue=8e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55783; Mature: 55783

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHEQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
HHHHHHHCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHEQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
HHHHHHHCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]