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The map label for this gene is topB [H]

Identifier: 117919934

GI number: 117919934

Start: 1729880

End: 1731994

Strand: Direct

Name: topB [H]

Synonym: Shewana3_1486

Alternate gene names: 117919934

Gene position: 1729880-1731994 (Clockwise)

Preceding gene: 117919931

Following gene: 117919935

Centisome position: 34.79

GC content: 51.35

Gene sequence:

>2115_bases
ATGATCCTCTATATTGCTGAAAAACCAAGCCTTGGGCGTGCAATTGCCGATGTGCTGCCTAAACCCCACAAAAAAGGTGA
CGGGTTTATTGAAGACGCCCAGGGCAACTGTGTGTCTTGGTGTGTGGGGCACTTGCTCGAACAGGCCGAGCCCGATGCCT
ATAATCCCGAGTTTAAATCGTGGAAGTTTGAGCATTTACCCATAGTGCCCGAGAAGTGGCAGCTTAAACCTAAGGCCGCG
ACCCGAAGCCAACTCACAGTACTTAAAAAACTGGTTAAACAGGCCGATACGCTAGTGAACGCTGGCGATCCCGACCGTGA
AGGGCAGCTATTGGTTGATGAAGTGATCGCCTATTTGGGCGTGACGGGCGATAAATTACAGCAAACGCAGCGACTCCTTA
TCAGTGACTTAAACCCGCAGGCGGTGAAACGTGCCCTGTCGCAGCTACGCAGTAACCGTGAGTTTATTCCTTTGTCGACT
TCGGCACTTGCCCGAAGCCGCGCCGATTGGCTCTATGGCATGAATATGACTCGGGCCTACACTATTCAAGGGAAAAAGGT
CGGCTATCAAGGCGTACTATCGGTCGGTCGAGTACAGACACCACTGCTTGGGCTTATCGTGCGCCGCGATGAAGAAATCG
CGAACTTTCAATCTAAGCCTTTTTATGAGGTTCTGGCTCACTTAGTGACGGACAAAGATCAGACTTTTACGGCGAAGTGG
CAACCCAGTGAAGCTTGTTTACCCTATATGGATGAGGAAGGGCGCGTGCTCGCTAAGGGCTTAGCGCAAAATGTGGTGCG
GCGGATCACCGATAAACCCGCCAAAGTAACCCAGTTAAGCACTAAGGATAAGCAGCAGAATCCGCCTTTACCCTACAGTT
TATCGGCGCTGCAAATCGATGCCGCCAAGCGCTTTGGTATGAGCGCGAAGGACGTGCTCGATACCTGCCAGAGCTTATAC
GAGCGCCATAAGCTAATCACCTATCCACGCTCAGACAGCCGTTATTTGCCCACAGAGCAGCATGCGCTTGCAAGCGGGGT
ATTAGCGGCCATCATGGGTGGTGCGCCCGAGTTAGTCGCACACGCTGAGGCGCCGAATCCTCGGCTTAAATCTAAGGCGT
GGGATGATAAAAAAGTCGATGCCCATCACGCCATTATTCCAACGGAAAAACAGCCCAATCTAGCTGGTTTAAGCCAGCGG
GAAAGACAGCTTTATCTGCATATTGCTCGCCAGTATTTGGCACAGTTTTACCCCGCCTATTGCTACAGTGAAACTGCGGT
CGAGGTGACGATCGAAGGTGGTCTTTTTAAAACTAAGGCAAAACAGGATAAGTCCCTCGGTTGGAAACAACTGTTTGCCA
GAGAAGAGAACGAACGAAAAGACGCATCATCCGCAGCGAGTTTTGCGCAAGGCGATAAGGACGCAGAGGAAGGAGAATAT
GTTGGTCAACTACCGCCGCTTACCTTAGGGCAAGTGCTTCATTGTCACCAAGGTGAATTAGTCGAGAAGCAAACTCAACC
CCCCAAAGCCTTTACCGATGCGACCTTGCTGAGTGCTATGACAGGGATTAACCGTTATGTGACAGATCCCGAGATCCGCA
AAATTTTAAAGGAAACCGATGGCTTAGGTACGGAAGCGACTCGCGCAGGCATTATTGAGCTGCTGTTTAAGCGAGGCTTT
ATTCAGCGTCAGGGGAAGTCGATTGTGGCCACTGAGATAGGCAAGGGTTTAATCAATAGTTTACCCATTAGCGCCACGAC
ACCCGATATGACGGCGCTGTGGGAGGCAAGCCTAAACGGTATCTGCCATAAAGAAATCTCATATCACGGGTTTATGCAGC
CACTGCTGGGGACGCTAAATAACTTAATCGAAAATGCAGGCGCTCAATTACCCTCAGCCCTCAATGGCTTAAAAGGGCAG
GGCTTTAAGCGTGCCGCGACTAAAAAGTCCGGTTATCGAAAAGCCGGAACCAGAGGCGCTGCAGGCGGGAAAAAAGCCGG
AACGGGCGCTAAAAAAGGGAATGCTAGCAATTCGGCCGCAGCATCTTCTACCTCCACGACAGCTAAACCGACTGCCCCTA
GGCGGCGAACGAGCAAAGTGCCTGCCCAAGCTTGA

Upstream 100 bases:

>100_bases
AGAGCGAGTTAAATCGGCCATCAAGATAAAACGGCGGTTTGCACTCTTGTCTGCTTTGGGCTAAATTACACTGTAATTAT
ATCCAGTGTTTAATGTAGAT

Downstream 100 bases:

>100_bases
TGACACATATGTGATCAAACTGTCGCTAAACCACGGGATTTGGTAACGATATTTAATTTATTCCGCGATATTTAATTACC
GCCAATCACGATATTTCGTG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDAQGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQTFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHALASGVLAAIMGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDAEEGEY
VGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEIGKGLINSLPISATTPDMTALWEASLNGICHKEISYHGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGTRGAAGGKKAGTGAKKGNASNSAAASSTSTTAKPTAPRRRTSKVPAQA

Sequences:

>Translated_704_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDAQGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQTFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHALASGVLAAIMGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDAEEGEY
VGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEIGKGLINSLPISATTPDMTALWEASLNGICHKEISYHGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGTRGAAGGKKAGTGAKKGNASNSAAASSTSTTAKPTAPRRRTSKVPAQA
>Mature_704_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDAQGNCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPEKWQLKPKAA
TRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLGVTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQTFTAKW
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPTEQHALASGVLAAIMGGAPELVAHAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQR
ERQLYLHIARQYLAQFYPAYCYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDAEEGEY
VGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETDGLGTEATRAGIIELLFKRGF
IQRQGKSIVATEIGKGLINSLPISATTPDMTALWEASLNGICHKEISYHGFMQPLLGTLNNLIENAGAQLPSALNGLKGQ
GFKRAATKKSGYRKAGTRGAAGGKKAGTGAKKGNASNSAAASSTSTTAKPTAPRRRTSKVPAQA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=637, Percent_Identity=23.2339089481947, Blast_Score=91, Evalue=5e-18,
Organism=Escherichia coli, GI1788061, Length=672, Percent_Identity=55.3571428571429, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=551, Percent_Identity=24.1379310344828, Blast_Score=103, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=643, Percent_Identity=23.950233281493, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585251, Length=535, Percent_Identity=24.4859813084112, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77182; Mature: 77182

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDAQGNCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPEKWQLKPKAATRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQTFTAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEEECC
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEECHHEEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPTEQHALASGVLAAIMGGAPELVA
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHE
HAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQRERQLYLHIARQYLAQFYPAY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDAEEGEY
HCCCCEEEEEECCCEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCC
VGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETD
CCCCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLGTEATRAGIIELLFKRGFIQRQGKSIVATEIGKGLINSLPISATTPDMTALWEASLNG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ICHKEISYHGFMQPLLGTLNNLIENAGAQLPSALNGLKGQGFKRAATKKSGYRKAGTRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHCCCHHCCCCCC
AGGKKAGTGAKKGNASNSAAASSTSTTAKPTAPRRRTSKVPAQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEDAQGNCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPEKWQLKPKAATRSQLTVLKKLVKQADTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLQQTQRLLISDLNPQAVKRALSQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLIVRRDEEIANFQSKPFYEVLAHLVTDKDQTFTAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEEECC
QPSEACLPYMDEEGRVLAKGLAQNVVRRITDKPAKVTQLSTKDKQQNPPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEECHHEEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPTEQHALASGVLAAIMGGAPELVA
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHE
HAEAPNPRLKSKAWDDKKVDAHHAIIPTEKQPNLAGLSQRERQLYLHIARQYLAQFYPAY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CYSETAVEVTIEGGLFKTKAKQDKSLGWKQLFAREENERKDASSAASFAQGDKDAEEGEY
HCCCCEEEEEECCCEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCC
VGQLPPLTLGQVLHCHQGELVEKQTQPPKAFTDATLLSAMTGINRYVTDPEIRKILKETD
CCCCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLGTEATRAGIIELLFKRGFIQRQGKSIVATEIGKGLINSLPISATTPDMTALWEASLNG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ICHKEISYHGFMQPLLGTLNNLIENAGAQLPSALNGLKGQGFKRAATKKSGYRKAGTRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHCCCHHCCCCCC
AGGKKAGTGAKKGNASNSAAASSTSTTAKPTAPRRRTSKVPAQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]