Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 117919868

GI number: 117919868

Start: 1649381

End: 1651009

Strand: Direct

Name: ada [H]

Synonym: Shewana3_1420

Alternate gene names: 117919868

Gene position: 1649381-1651009 (Clockwise)

Preceding gene: 117919866

Following gene: 117919869

Centisome position: 33.17

GC content: 51.32

Gene sequence:

>1629_bases
ATGAAGCAGACAAGCGTGAGCCAAAATTCAGCCCCACAACCGCCATCCGAGCCGACATCAAGCGACGCGCAGGATTGCCA
AATATCCGCCGGGATTTGCCGTGAAGCGCGCATGAGCCGCGATCCACGTTTTGACGGTAAGTTCTTTGTGGGCGTGTTAA
GTACGGGGATTTATTGCCGAACAGTGTGCCCAGCAGTAGCGCCGAAGGAAGAAAATGTGCGTTATTTTGACTCGGCCATT
AAAGCTGCACAGGCGGGACTCAGGCCTTGTCTACGTTGCCGCCCCGACAGTGCTCCTGGCTCTAATCCGTGGAAAGGCAC
TAACACAACCCTAGACAGAGCCATAGGTTTGATTGAAGCTGGCGCCTTATCTGGTGAGCACGGATTAACGGTCGAGGCCT
TGGCGGACAAATTGGGGATCAGCAGCCGTTATTTAAATAAATTATTTACCGCAGGATTTGGCACTTCACCTAAACAATTT
GCCCTCTATCGTCAGTTATTGTTCGCAAAGCAGTTGTTGCATCAGACGCAGTTGCCCATCACTCAGGTCGCCCTTGCCGC
AGGCTTTAACAGTATTCGCCGTTTTAACGAGGCATTTCAGCAGGCGCTGCAATTAACGCCCACCCAGTTGCGAAAATCCA
GTCAGAAATCGACTATTAAAATAGACGACGGGGAGAGTACTGAACTCGCTTGTTCACAGGAGATGCCAGCGCACAGCTTG
AGTCTGTATCAGTATTATCGACCGCCGCTCGATTGGTCGGCGCAATTAGCCTTTTATCGCCTGCGCGCCGTCGAAGGCAT
GGAATGGTTTAGTGAGAGTGTAGCCCCTCACTTCCATGAGGCGATTGACCCAAATATACCACTTGAGTATGGCCGCACCC
TGCAAATTGAGGATATTCGCGCAGTGGTACATATCGTCCATGAGGCGCCTTTGCATCGCTTTAAAATCACGCTGACGTTA
ACGCCTGACTCGCCCTTATCGGGTCTACAAAAGCTCATCACCCAAGTGCGGCGCATTTTAGATCTCGATGCAGATATGCA
GAAGATTGAACACAACCTTCAGCATTTGACCGATCTTAAACTCAGTAGCAAATCCGGGCTTAGGATCCCCGGCGCAGGCG
CGGTGTTTGAGGCGGGTTGCCGAGCCGTCTTAGGTCAGCAGGTGAGCGTAGTGCAGGCGACTAAATTACTGAATATATTG
GTCGAAGCCTATGGCGAGTGCTTTAGTTTAAACGGACGGGAATATCGCCTGTTTCCTACGCCGCAGGCGATTCGTGATGC
GAGTCTCGATGAGCTTAAAATGCCCGGTGCCCGCAAACTGGCGCTCAATGCGCTTGCAGCCTTTATCTGTGAGCAGCCCG
AAGCCTCAGTCGATGAGTGGATTGGGGTAAAAGGCATAGGCCCTTGGACCATTGCCTACGCCAAATTACGTGGACTTGGC
GATCCGAATATCTTCTTGCACACGGATTTAATTGTTAAAAAACAATTGTTAGCCTGTGTTGCCGAGCAAAGTGGCTTGGA
TATTGAAACGGTAAAACAGCTGGATTATACGGCGCTTGCACAGCGGGTGAGTGCGGATATCGCCCCTTGGGGAAGTTATT
TGACCTTCCAGCTGTGGTCCAATGCATAG

Upstream 100 bases:

>100_bases
ACGCGATGATCCAGTTCCGTTTTCCGCGAGTGTGCTAGCATAGGGTGACGTACAGTAACCGACAAGTACAAAATTGTGAA
TAAGAGTGTCGGATAGCCAA

Downstream 100 bases:

>100_bases
GCATTTCAGGCCACTCGTTATTGTTCCAGTGACTAGGAACCAGTGACGAGGCTCCATATGAGAGCCACTATTAACTATTT
GAAACACAGTACGAAAACAC

Product: transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKQTSVSQNSAPQPPSEPTSSDAQDCQISAGICREARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGALSGEHGLTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTIKIDDGESTELACSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVEGMEWFSESVAPHFHEAIDPNIPLEYGRTLQIEDIRAVVHIVHEAPLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQKIEHNLQHLTDLKLSSKSGLRIPGAGAVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGECFSLNGREYRLFPTPQAIRDASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLACVAEQSGLDIETVKQLDYTALAQRVSADIAPWGSYLTFQLWSNA

Sequences:

>Translated_542_residues
MKQTSVSQNSAPQPPSEPTSSDAQDCQISAGICREARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGALSGEHGLTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTIKIDDGESTELACSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVEGMEWFSESVAPHFHEAIDPNIPLEYGRTLQIEDIRAVVHIVHEAPLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQKIEHNLQHLTDLKLSSKSGLRIPGAGAVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGECFSLNGREYRLFPTPQAIRDASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLACVAEQSGLDIETVKQLDYTALAQRVSADIAPWGSYLTFQLWSNA
>Mature_542_residues
MKQTSVSQNSAPQPPSEPTSSDAQDCQISAGICREARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGALSGEHGLTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTIKIDDGESTELACSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVEGMEWFSESVAPHFHEAIDPNIPLEYGRTLQIEDIRAVVHIVHEAPLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQKIEHNLQHLTDLKLSSKSGLRIPGAGAVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGECFSLNGREYRLFPTPQAIRDASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLACVAEQSGLDIETVKQLDYTALAQRVSADIAPWGSYLTFQLWSNA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=310, Percent_Identity=29.0322580645161, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=185, Percent_Identity=31.8918918918919, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 59737; Mature: 59737

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTSVSQNSAPQPPSEPTSSDAQDCQISAGICREARMSRDPRFDGKFFVGVLSTGIYCR
CCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
TVCPAVAPKEENVRYFDSAIKAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GALSGEHGLTVEALADKLGISSRYLNKLFTAGFGTSPKQFALYRQLLFAKQLLHQTQLPI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTIKIDDGESTELACSQEMPAHSL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCCEEECCCCCCHHH
SLYQYYRPPLDWSAQLAFYRLRAVEGMEWFSESVAPHFHEAIDPNIPLEYGRTLQIEDIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEHHHHH
AVVHIVHEAPLHRFKITLTLTPDSPLSGLQKLITQVRRILDLDADMQKIEHNLQHLTDLK
HHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
LSSKSGLRIPGAGAVFEAGCRAVLGQQVSVVQATKLLNILVEAYGECFSLNGREYRLFPT
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCC
PQAIRDASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
CHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DPNIFLHTDLIVKKQLLACVAEQSGLDIETVKQLDYTALAQRVSADIAPWGSYLTFQLWS
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
NA
CC
>Mature Secondary Structure
MKQTSVSQNSAPQPPSEPTSSDAQDCQISAGICREARMSRDPRFDGKFFVGVLSTGIYCR
CCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
TVCPAVAPKEENVRYFDSAIKAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GALSGEHGLTVEALADKLGISSRYLNKLFTAGFGTSPKQFALYRQLLFAKQLLHQTQLPI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTIKIDDGESTELACSQEMPAHSL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCCEEECCCCCCHHH
SLYQYYRPPLDWSAQLAFYRLRAVEGMEWFSESVAPHFHEAIDPNIPLEYGRTLQIEDIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEHHHHH
AVVHIVHEAPLHRFKITLTLTPDSPLSGLQKLITQVRRILDLDADMQKIEHNLQHLTDLK
HHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
LSSKSGLRIPGAGAVFEAGCRAVLGQQVSVVQATKLLNILVEAYGECFSLNGREYRLFPT
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCC
PQAIRDASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
CHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DPNIFLHTDLIVKKQLLACVAEQSGLDIETVKQLDYTALAQRVSADIAPWGSYLTFQLWS
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]