Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 117919866

GI number: 117919866

Start: 1643838

End: 1645247

Strand: Direct

Name: gltX [H]

Synonym: Shewana3_1418

Alternate gene names: 117919866

Gene position: 1643838-1645247 (Clockwise)

Preceding gene: 117919859

Following gene: 117919868

Centisome position: 33.06

GC content: 45.89

Gene sequence:

>1410_bases
ATGACAACTAAGACGCGTTTTGCCCCCAGCCCAACAGGCTTTTTGCACGTGGGCGGTGCCCGTACAGCTCTTTATTCTTG
GTTACAAGCTCGTGCCAATAATGGTGAGTTTGTTTTACGTATCGAAGATACGGATATTGAACGTTCAACTCAGGCTGCCT
GTGATGCCATTTTAGAGGGCATGAACTGGTTAGGTCTGACGTGGGACGAGGGCCCGTACTATCAAACTAAGCGTTTTGAT
CGTTATAACGAAATCATCGCGCAGATGTTAGAGCAGGGCACAGCTTATAAATGTTATTGCTCGCGTGAACGTATCGATGC
TTTAAGAGAAGCACAAGCGGCAAATGGCGAAGCCCAAAAATACGATGGTTGCTGCCGTGACTTGCCGGCGCGTGACACTG
ATGAGCCTTTTGTTGTGCGTTTTAAAAACCCCATCGGCGGTTCAGTGGTATTTGATGACCATGTCCGTGGCCGTATCGAG
TTTTCAAACGATGCATTAGATGATCTGATCATTGCCCGTACCGATGGCGTACCGACTTATAACTTCTGTGTGGTTGTTGA
CGATTGGGATATGGGTATTACCTGTGTGGTTCGCGGTGAAGACCATATCAACAACACGCCACGTCAAATCAACATCCTTA
AAGCATTAGGCGCGCCTATTCCTGAATATGCCCACGTGTCGATGATTTTAGGGGATGACGGTGCCAAGTTGTCTAAGCGT
CACGGTGCGGTAAGCGTAATGCAATACCGTGATGATGGTTACCTACCAGAAGCACTGCTTAACTACTTAGTTCGCTTAGG
TTGGTCACATGGCGATCAAGAAGTGTTCTCTTTAGAAGAAATGAAGCAGCTCTTTAAGTTAGATGACATCAACAAAGCGC
CTTCTGCTTTTAATACCGAAAAACTGGTTTGGTTAAACCAACACTATATTAAGACGCTTGATCCTGAATATGTGGCTAGC
CATTTACAGTGGCACATGGACGATCAAAAGATTGATACCTCAAATGGCCCTGCTTTATCTGCGGTAGTAACAGCATTGGC
TGAACGCGCTAAAACTTTAAAAGAGTTAGCCGCTTCTAGCCGCTACTTCTATGAAGACTTCGCAGAGTTCGATGCTGAGC
AAGCGAAAAAGCACTTACGTGGTGTCGCGCTTGAGCCATTACAATTAGTGCAACAAAAACTGGCTGCATTAACTGAGTGG
ACTGTTGAAGCTATCCACCAAGCGATTGAAGCTACCGCGACCGAATTAGAAGTCGGTATGGGTAAAGTGGGTATGCCATT
ACGTGTCGCTGTGACAGGTGCAGGGCAGTCTCCTGGCTTAGATATCACCTTATTCTTAATAGGAAAGGCGCGTTCTGAGC
AAAGAATATCCAAAGCGATTGAATTTGTAGCAGATAGAATAAATTCCTAA

Upstream 100 bases:

>100_bases
TCAGTGCCATTTGCAGCGCAGACTTAGGCTGCTAGAATAAGGCCACTTTTTACCGTGATTGTCTTTATCTAGACGACTTT
ATATAGGTTAGTGTCCCATT

Downstream 100 bases:

>100_bases
AAAACGAGTTGACACATTTAGGTGCGCTGCATAGAATGCGCCCCGTAGTTGAGACACAGACGAGCGAATGCTTGTAGTGC
ACTTAAATACAGCTTGATAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQLFKLDDINKAPSAFNTEKLVWLNQHYIKTLDPEYVAS
HLQWHMDDQKIDTSNGPALSAVVTALAERAKTLKELAASSRYFYEDFAEFDAEQAKKHLRGVALEPLQLVQQKLAALTEW
TVEAIHQAIEATATELEVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGKARSEQRISKAIEFVADRINS

Sequences:

>Translated_469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQLFKLDDINKAPSAFNTEKLVWLNQHYIKTLDPEYVAS
HLQWHMDDQKIDTSNGPALSAVVTALAERAKTLKELAASSRYFYEDFAEFDAEQAKKHLRGVALEPLQLVQQKLAALTEW
TVEAIHQAIEATATELEVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGKARSEQRISKAIEFVADRINS
>Mature_468_residues
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFDR
YNEIIAQMLEQGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEF
SNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKRH
GAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQLFKLDDINKAPSAFNTEKLVWLNQHYIKTLDPEYVASH
LQWHMDDQKIDTSNGPALSAVVTALAERAKTLKELAASSRYFYEDFAEFDAEQAKKHLRGVALEPLQLVQQKLAALTEWT
VEAIHQAIEATATELEVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGKARSEQRISKAIEFVADRINS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=332, Percent_Identity=34.0361445783133, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=73.3475479744137, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=40.0826446280992, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=24.3119266055046, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=30.3157894736842, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=32, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.9475982532751, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=118, Percent_Identity=29.6610169491525, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=29.4589178356713, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52475; Mature: 52344

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIDALREAQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQLFKLDDINKAPSAFNTE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KLVWLNQHYIKTLDPEYVASHLQWHMDDQKIDTSNGPALSAVVTALAERAKTLKELAASS
EEEEECCHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFAEFDAEQAKKHLRGVALEPLQLVQQKLAALTEWTVEAIHQAIEATATELEVGM
CHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGQSPGLDITLFLIGKARSEQRISKAIEFVADRINS
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIDALREAQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSLEEMKQLFKLDDINKAPSAFNTE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KLVWLNQHYIKTLDPEYVASHLQWHMDDQKIDTSNGPALSAVVTALAERAKTLKELAASS
EEEEECCHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFAEFDAEQAKKHLRGVALEPLQLVQQKLAALTEWTVEAIHQAIEATATELEVGM
CHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGQSPGLDITLFLIGKARSEQRISKAIEFVADRINS
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA