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The map label for this gene is nusA [H]

Identifier: 117919478

GI number: 117919478

Start: 1200689

End: 1202188

Strand: Direct

Name: nusA [H]

Synonym: Shewana3_1029

Alternate gene names: 117919478

Gene position: 1200689-1202188 (Clockwise)

Preceding gene: 117919477

Following gene: 117919479

Centisome position: 24.15

GC content: 48.13

Gene sequence:

>1500_bases
ATGAATAAAGAGATTCTGCTAGTCGCTGAGGCGGTTTCAAATGAAAAAGCCGTTCCACGCGAGAAAATTTTTGAAGCGCT
AGAAACTGCGCTGGCAACTGCAACCAAGAAAAAATACGAAGGCGATATCGACGTTCGTGTTTCTATCGACCGTAAAACCG
GCGCCTATGAAACGTTCCGCCGCTGGATGGTGATCGATGATAACGGTGTTGCGTTAGAAAACCCATACCGCGAAATCACC
TTGGAAGCTGCGCGTTACGAAAACCCAGAAATCCAACCTGGCGATTACATCGAAGATCAGATCGAATCTGTTGCCTTCGA
CCGTATTACGACGCAAACCGCTAAGCAAGTGATCGTACAAAAAGTACGTGAAGCCGAGCGTGCGCAAGTCGTTGAACAGT
TCCAAGATAAAGAAGGCGAGCTGATCACTGGTGTTGTGAAAAAAGCGACCCGTGAAAGTGTTGTTGTTGACTTAGGTAAC
AACGCTGACGGCGTATTGTTCCGTGAAGATTTAATTTCACGCGAATCTTTCCGTCCTGGTGACCGTGTCCGTGCACTGCT
GTATTCAGTACGTCCAGAGGCACGTGGCGCGCAGTTATTCTTAACTCGCACTAAGCCAGATATGCTGATTGAGCTGTTCC
GTGTTGAAGTACCGGAAATCGCCGATGAGCTGATCGAAGTCATGGGCGCTGCGCGCGACCCAGGTGCACGCGCTAAGATC
GCCGTGAAGTCAAACGACCGTCGTATCGACCCAATCGGTGCTTGCGTAGGTATGCGTGGTGCACGTGTACAAGCAGTATC
GAATGAACTCGGTGGCGAGCGTGTGGATATCGTGTTGTGGGATGATAATCCAGCACAATATGTGATCAACGCAATGGCGC
CTGCCGATGTGGCTTCTATCATCGTTGATGAAGACAACCACTCAATGGATATCGCCGTTGAAGCGGAAAGTCTGGCACAA
GCGATTGGCCGTAACGGTCAAAACGTACGTTTAGCGACTCAGCTGACTGGTTGGGAACTGAACGTAATGACAGTGGAAGA
CATGAACAAGAAGCACCAAGCCGAGAGCGCTAAGGTGAAGAGCTTATTCATTAACGCACTGGATGTAGATGAAGATTTTG
CTCAAGTACTGGCTGACGAAGGTTTTACTTCTCTCGAAGAAGTGGCTTACGTGCCTGTATCTGAATTATTAGCAATTGAT
GGCTTCGATGAAGATATCGTTGAAGCATTACGTGAGCGTGCAAAAGCGGCGATCTCAACTCGAGCTCTCGCCACTGAAGA
AGCACTAGATGGTGTGGAGCCAAGTGAAGAGTTATTAGCACTTGAAGGTGTTGAAAGACACTTAGCCTATGTTTTAGCAA
GCAAAGGTATCGTGACTCTAGAAGACTTGGCTGAACAAGGCATTGATGATTTGATCGAAATTGAAGAATTGACAGAAGAA
AAAGCAGGTGCGCTGATCATGGCTGCCCGCAACATCTGTTGGTTTGGCGAAGAAGCATAA

Upstream 100 bases:

>100_bases
ATGTGAATGGTAAAGAGCTGGTTGTCGCCTTGGATAATATCCGTAAAGGCAACATCATCGCTAAGTTTTGATGGTTTCAA
GGTGAACGAGGCAAGACGAA

Downstream 100 bases:

>100_bases
GTCGATCAACAGGGGGATTTAACTGATGGCAGATACTACCGTAGAGAAATTGGCCACGGAAGTGGGTAAAAGTGTTGAAC
GTCTGATTGAGCAATTCTCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVSIDRKTGAYETFRRWMVIDDNGVALENPYREIT
LEAARYENPEIQPGDYIEDQIESVAFDRITTQTAKQVIVQKVREAERAQVVEQFQDKEGELITGVVKKATRESVVVDLGN
NADGVLFREDLISRESFRPGDRVRALLYSVRPEARGAQLFLTRTKPDMLIELFRVEVPEIADELIEVMGAARDPGARAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQYVINAMAPADVASIIVDEDNHSMDIAVEAESLAQ
AIGRNGQNVRLATQLTGWELNVMTVEDMNKKHQAESAKVKSLFINALDVDEDFAQVLADEGFTSLEEVAYVPVSELLAID
GFDEDIVEALRERAKAAISTRALATEEALDGVEPSEELLALEGVERHLAYVLASKGIVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEEA

Sequences:

>Translated_499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVSIDRKTGAYETFRRWMVIDDNGVALENPYREIT
LEAARYENPEIQPGDYIEDQIESVAFDRITTQTAKQVIVQKVREAERAQVVEQFQDKEGELITGVVKKATRESVVVDLGN
NADGVLFREDLISRESFRPGDRVRALLYSVRPEARGAQLFLTRTKPDMLIELFRVEVPEIADELIEVMGAARDPGARAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQYVINAMAPADVASIIVDEDNHSMDIAVEAESLAQ
AIGRNGQNVRLATQLTGWELNVMTVEDMNKKHQAESAKVKSLFINALDVDEDFAQVLADEGFTSLEEVAYVPVSELLAID
GFDEDIVEALRERAKAAISTRALATEEALDGVEPSEELLALEGVERHLAYVLASKGIVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEEA
>Mature_499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVSIDRKTGAYETFRRWMVIDDNGVALENPYREIT
LEAARYENPEIQPGDYIEDQIESVAFDRITTQTAKQVIVQKVREAERAQVVEQFQDKEGELITGVVKKATRESVVVDLGN
NADGVLFREDLISRESFRPGDRVRALLYSVRPEARGAQLFLTRTKPDMLIELFRVEVPEIADELIEVMGAARDPGARAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQYVINAMAPADVASIIVDEDNHSMDIAVEAESLAQ
AIGRNGQNVRLATQLTGWELNVMTVEDMNKKHQAESAKVKSLFINALDVDEDFAQVLADEGFTSLEEVAYVPVSELLAID
GFDEDIVEALRERAKAAISTRALATEEALDGVEPSEELLALEGVERHLAYVLASKGIVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEEA

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=499, Percent_Identity=73.1462925851703, Blast_Score=752, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55185; Mature: 55185

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVSIDRKTGAYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
RWMVIDDNGVALENPYREITLEAARYENPEIQPGDYIEDQIESVAFDRITTQTAKQVIVQ
EEEEECCCCCEECCCHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVREAERAQVVEQFQDKEGELITGVVKKATRESVVVDLGNNADGVLFREDLISRESFRPG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEHHHHHCCCCCCCH
DRVRALLYSVRPEARGAQLFLTRTKPDMLIELFRVEVPEIADELIEVMGAARDPGARAKI
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEE
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQYVINAMAPADVASI
EEECCCCCCCHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHEECCCCCCEEEE
IVDEDNHSMDIAVEAESLAQAIGRNGQNVRLATQLTGWELNVMTVEDMNKKHQAESAKVK
EEECCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHCCHHHHHHHHHHH
SLFINALDVDEDFAQVLADEGFTSLEEVAYVPVSELLAIDGFDEDIVEALRERAKAAIST
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHHH
RALATEEALDGVEPSEELLALEGVERHLAYVLASKGIVTLEDLAEQGIDDLIEIEELTEE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHH
KAGALIMAARNICWFGEEA
HCCEEEEEECCEEECCCCC
>Mature Secondary Structure
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVSIDRKTGAYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
RWMVIDDNGVALENPYREITLEAARYENPEIQPGDYIEDQIESVAFDRITTQTAKQVIVQ
EEEEECCCCCEECCCHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVREAERAQVVEQFQDKEGELITGVVKKATRESVVVDLGNNADGVLFREDLISRESFRPG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEHHHHHCCCCCCCH
DRVRALLYSVRPEARGAQLFLTRTKPDMLIELFRVEVPEIADELIEVMGAARDPGARAKI
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEE
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQYVINAMAPADVASI
EEECCCCCCCHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHEECCCCCCEEEE
IVDEDNHSMDIAVEAESLAQAIGRNGQNVRLATQLTGWELNVMTVEDMNKKHQAESAKVK
EEECCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHCCHHHHHHHHHHH
SLFINALDVDEDFAQVLADEGFTSLEEVAYVPVSELLAIDGFDEDIVEALRERAKAAIST
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHHH
RALATEEALDGVEPSEELLALEGVERHLAYVLASKGIVTLEDLAEQGIDDLIEIEELTEE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHH
KAGALIMAARNICWFGEEA
HCCEEEEEECCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]