Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 117919437

GI number: 117919437

Start: 1158903

End: 1159868

Strand: Reverse

Name: lipA [H]

Synonym: Shewana3_0988

Alternate gene names: 117919437

Gene position: 1159868-1158903 (Counterclockwise)

Preceding gene: 117919438

Following gene: 117919434

Centisome position: 23.33

GC content: 51.45

Gene sequence:

>966_bases
ATGAATAGGCCTGAACGTTTACAACCCGGAGTCAAATTAAGAGATGCCGATAAGGTCTCGCGCATTCCGGTTAAAATCGT
GCCCTCTGAGCGCGAAACCATGTTACGCAAACCCGATTGGTTAAGGGTCAAACTGCCCGCATCTAACCAACGCATTCTCG
AGATCAAGCAAGCATTACGTTCGAACGGTCTGCACTCGGTCTGTGAAGAAGCTTCTTGCCCTAACTTGGCAGAATGTTTT
AACCACGGCACCGCGACCTTTATGATTTTGGGCGCAATTTGTACTCGCCGCTGCCCATTCTGCGACGTGGCCCACGGTCG
TCCATTGAAACCTGATGCAGAAGAGCCGGTCAAACTGGCACAAACCATTCGCGATATGAAGCTGAAATACGTGGTTATCA
CCTCGGTTGACCGCGATGACCTGCGTGATGGCGGTGCCCAGCACTTTGCCGACTGTATCCGTGAGATCCGTAAACTGAAT
CCTGAAATTAAGATTGAGATCTTAGTGCCCGATTTCCGTGGTCGTATCGACGCTGCCCTCGACATTCTGTCGACCGAGCC
GCCTGATGTGTTCAACCACAACCTCGAAACTGCGCCTATGCATTACCGCAAAGCGCGTCCAGGGGCGAACTATCAATGGT
CACTCGACCTACTGAAGCGCTTTAAAGAGCGTCACCCCAATGTGCCGACAAAATCGGGACTAATGATGGGTCTAGGTGAA
ACCAACGAAGAAATCGCCCAAGTGCTGCGCGACTTACGTGAGCACAAGGTAGAAATGTTGACCTTAGGTCAATATCTGCA
ACCGTCTAAGTTCCACCTGCCGGTTGAGCGTTACGTCTCGCCAGCCGAGTTCGATGAGCTTAAAGTCCTTGCCGATGAGC
TGGGCTTTACTCACGCCGCCTGTGGCCCATTAGTACGTTCAAGCTACCATGCAGACCTGCAAGCTCAAGGTAAAGAAGTG
AAGTAA

Upstream 100 bases:

>100_bases
GCCCACAAACCGTGATAGAAGCAGGCGACCAACTCATAATTACCCTTAGCCAACTATTGGGCTACCAACAACTAGTTCAT
CATCAAGGATTAGCAGCGTC

Downstream 100 bases:

>100_bases
CCTTCGCTTTATTAACTCCATAAAAAAACAGCGCTAAAGCGCTGTTTTTTTTATCGACTTCTAAAATCTTAAGCCGTTTC
GCTTAAGGCTAAGTCCATCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERETMLRKPDWLRVKLPASNQRILEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PEIKIEILVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLREHKVEMLTLGQYLQPSKFHLPVERYVSPAEFDELKVLADELGFTHAACGPLVRSSYHADLQAQGKEV
K

Sequences:

>Translated_321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERETMLRKPDWLRVKLPASNQRILEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PEIKIEILVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLREHKVEMLTLGQYLQPSKFHLPVERYVSPAEFDELKVLADELGFTHAACGPLVRSSYHADLQAQGKEV
K
>Mature_321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERETMLRKPDWLRVKLPASNQRILEIKQALRSNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PEIKIEILVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLREHKVEMLTLGQYLQPSKFHLPVERYVSPAEFDELKVLADELGFTHAACGPLVRSSYHADLQAQGKEV
K

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=45.4225352112676, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=44.758064516129, Blast_Score=211, Evalue=5e-55,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=75.3894080996885, Blast_Score=517, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32564533, Length=305, Percent_Identity=40.9836065573771, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=305, Percent_Identity=43.6065573770492, Blast_Score=241, Evalue=9e-65,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=45.6747404844291, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36466; Mature: 36466

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPERLQPGVKLRDADKVSRIPVKIVPSERETMLRKPDWLRVKLPASNQRILEIKQALR
CCCCCCCCCCCEECCCCHHHCCCEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
SNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLNPEIKIEILVPDFRGRIDAAL
HHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
DILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
HHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIAQVLRDLREHKVEMLTLGQYLQPSKFHLPVERYVSPAEFDELKVLADELGFTHAA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MNRPERLQPGVKLRDADKVSRIPVKIVPSERETMLRKPDWLRVKLPASNQRILEIKQALR
CCCCCCCCCCCEECCCCHHHCCCEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
SNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLNPEIKIEILVPDFRGRIDAAL
HHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
DILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
HHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIAQVLRDLREHKVEMLTLGQYLQPSKFHLPVERYVSPAEFDELKVLADELGFTHAA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA