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The map label for this gene is lipB [H]

Identifier: 117919438

GI number: 117919438

Start: 1159865

End: 1160518

Strand: Reverse

Name: lipB [H]

Synonym: Shewana3_0989

Alternate gene names: 117919438

Gene position: 1160518-1159865 (Counterclockwise)

Preceding gene: 117919439

Following gene: 117919437

Centisome position: 23.34

GC content: 51.22

Gene sequence:

>654_bases
TTGCAAGACACCACTTTGCATATTCGGCATCTGGGACAACAGGATTACGAGTCTGTGTGGCATGCGATGCAGCATTACAC
CGACACACGTAACAGCGATAGCCCAGACGAACTGTGGATCGTTGAACATCCACCCGTATTTACCCAAGGCCAAGCAGGTA
AAAGCGAACATATTTTGAATCCGGGTGATATTCCCGTGATCCAAGTGGATCGTGGCGGCCAAGTGACTTACCACGGGCCG
GGACAATTAGTTGTCTACCCTCTCCTCGATATTAAACGCAGCAAGATTGGCGTCCGTCAGCTCGTGACCCATATCGAGCA
AAGCATTATCGATATGCTCGCCAAATACGATATCAATGCCTACGCCAAGGCCGATGCGCCGGGCGTGTATGTGGATCAGC
GCAAAGTCGCCTCTTTAGGGCTTCGCATTCGTAAGGGCTGCTCCTTCCACGGCTTAGCACTGAATGTCGATATGGATCTT
GCGCCGTTTCGCCGCATTAACCCTTGCGGTTATGCCGGGCTTGAAATGGTGCAATGCAAGGAACTCGGCGGCCCACAAAC
CGTGATAGAAGCAGGCGACCAACTCATAATTACCCTTAGCCAACTATTGGGCTACCAACAACTAGTTCATCATCAAGGAT
TAGCAGCGTCATGA

Upstream 100 bases:

>100_bases
TACTATAAGTATTCCTCAAGGCCATGAAATGTGATCTGTCTTACATTTATGGTCAAGAGGCGTATAATAGCGCCACTCAA
TTCTAGGGGAGAGGTTGCCC

Downstream 100 bases:

>100_bases
ATAGGCCTGAACGTTTACAACCCGGAGTCAAATTAAGAGATGCCGATAAGGTCTCGCGCATTCCGGTTAAAATCGTGCCC
TCTGAGCGCGAAACCATGTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQDTTLHIRHLGQQDYESVWHAMQHYTDTRNSDSPDELWIVEHPPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKIGVRQLVTHIEQSIIDMLAKYDINAYAKADAPGVYVDQRKVASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKELGGPQTVIEAGDQLIITLSQLLGYQQLVHHQGLAAS

Sequences:

>Translated_217_residues
MQDTTLHIRHLGQQDYESVWHAMQHYTDTRNSDSPDELWIVEHPPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKIGVRQLVTHIEQSIIDMLAKYDINAYAKADAPGVYVDQRKVASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKELGGPQTVIEAGDQLIITLSQLLGYQQLVHHQGLAAS
>Mature_217_residues
MQDTTLHIRHLGQQDYESVWHAMQHYTDTRNSDSPDELWIVEHPPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKIGVRQLVTHIEQSIIDMLAKYDINAYAKADAPGVYVDQRKVASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKELGGPQTVIEAGDQLIITLSQLLGYQQLVHHQGLAAS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=166, Percent_Identity=32.5301204819277, Blast_Score=67, Evalue=9e-12,
Organism=Escherichia coli, GI87081767, Length=202, Percent_Identity=60.3960396039604, Blast_Score=255, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6323268, Length=162, Percent_Identity=32.7160493827161, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355483, Length=186, Percent_Identity=29.5698924731183, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24097; Mature: 24097

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTTLHIRHLGQQDYESVWHAMQHYTDTRNSDSPDELWIVEHPPVFTQGQAGKSEHILN
CCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRSKIGVRQLVTHIEQSIIDMLAKYDINA
CCCCCEEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YAKADAPGVYVDQRKVASLGLRIRKGCSFHGLALNVDMDLAPFRRINPCGYAGLEMVQCK
EECCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
ELGGPQTVIEAGDQLIITLSQLLGYQQLVHHQGLAAS
HCCCCHHHHHCCCHHEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQDTTLHIRHLGQQDYESVWHAMQHYTDTRNSDSPDELWIVEHPPVFTQGQAGKSEHILN
CCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRSKIGVRQLVTHIEQSIIDMLAKYDINA
CCCCCEEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YAKADAPGVYVDQRKVASLGLRIRKGCSFHGLALNVDMDLAPFRRINPCGYAGLEMVQCK
EECCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
ELGGPQTVIEAGDQLIITLSQLLGYQQLVHHQGLAAS
HCCCCHHHHHCCCHHEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]