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The map label for this gene is parE [H]

Identifier: 117919182

GI number: 117919182

Start: 845650

End: 847536

Strand: Direct

Name: parE [H]

Synonym: Shewana3_0730

Alternate gene names: 117919182

Gene position: 845650-847536 (Clockwise)

Preceding gene: 117919181

Following gene: 117919183

Centisome position: 17.01

GC content: 50.24

Gene sequence:

>1887_bases
ATGACGAATCAATACACCTCTGACGCCATTGAAGTTCTTAACGGACTCGACCCAGTTAAACGCCGTCCAGGTATGTATAC
CGATACCACCCGACCTAACCACTTAGGCCAAGAGGTCATAGATAACAGTGTGGACGAGGCCTTAGCGGGCCACGCCACTA
AAATCGAAGTTGTGCTCCATACCGATAATTCCCTTGAAGTCACCGACGATGGTCGGGGGATGCCGGTAGATATTCACCCT
GAAGAAGGCATTCCCGGGGTCGAACTCATCCTTACTAAACTGCATGCCGGCGGTAAATTTTCGAATAAAAATTACCAATT
CTCCGGTGGTTTGCACGGTGTGGGGATTTCGGTGGTTAACGCTCTGTCTCGTCGCGTTGAAGTGACGGTACGCCGTGACG
GCCAAGTGTATGATATTGCTTTTGAAAATGGCTTTAAAGTTGAAGAATTAAAAGTTACAGGTACTTGCGGTCGTCGCAAT
ACTGGTACCCGAGTACATTTCTGGCCCGACGCCAGCTATTTCGATTCGGCGAATTTCTCCAACTCCAAACTCATCTATTT
GCTGCGCGCCAAGGCCGTGTTGTGTCCGGGTCTTAGGATCAAATTTACTAATAAACACACCAACGAAGTCCATGAGTGGT
ACTACGAGAGTGGTTTAACCGATTATCTTAAGGCGGCGGTTGGCGATAATTTAATGCTGCCAGAAGAGCCCTTTATCGGC
AGCTTTAAAGGTCAAATCGAAGCCGCTGATTGGGCCATTACTTGGCTGCCTGAAGGTGGCGATGCGATCAATGAAAGCTA
TGTTAACTTGATCCCAACGCCATTGGGTGGAACCCATGTGAACGGTTTCCGCCAAGGTTTGCTGGAGTCGATGCGCGAGT
TCTGTGAGTTTCGCAATCTGATCCCCCGCGGCATCAAGCTGTCGCCTGAGGATATTTGGGATCGCGCCGCCTTCATTCTC
TCGGTGAAGATGCAAGATCCGCAGTTTGCCGGTCAAACCAAAGAGAAACTCTCGAGCCGTCAAAGCTCTGCCTTCGTCTC
TGGTATTGTGCGTGACGCGTTTTCACTGTGGCTCAACTCGAATACAGAATTGGCCGAGTCGCTGGCGGACATGTGTATTA
ACAACGCCCAGCGCCGCTTAAAAGCCTCGAAAAAAGTGGCCCGTAAAAAAGTCACCTCAGGCCCGGCATTACCGGGTAAA
TTGACCGACTGTAGCGGCCAAGATCCGATGCGCGGCGAACTCTTCTTAGTGGAAGGGGACTCTGCGGGCGGCAGTGCGAA
GCAGGCGCGCGACCGTGAGTTTCAGGCCATTATGCCGCTGCGGGGTAAAATCCTAAACACGTGGGAGGTGGATGCTTCAC
AGGTGTTGGCCTCGCAGGAAGTGCATGACATCTCGGTGGCGATTGGTTGCGATCCCGATAGCAACGATATCTCCGAGCTA
AGATATGGCAAGATTTGTATTCTCGCCGACGCCGACTCCGACGGGCTACATATTGCAACTTTACTCTGCGCCCTGTTTCT
GAAACATTACCGTGTGCTGGTCGAACAGGGCCATGTCTATATCGCTATGCCACCGCTGTTCCGTATCGATATAGGTAAAG
ATGTTTATTACGCCTTAGATGAGGCTGAAAAAGAAGGTATTCTCGACCGTATTACTGCCGAGAATAAAAAGGGTAAGGTC
CAAGTTACGCGCTTTAAAGGTCTGGGTGAGATGAACCCGCTGCAATTGCGTGAAACGACTATGGATCCAAACACTCGCCG
TTTAGTTCAGTTAACCATTGATGATGTAGAAGAAACTGACTCACTGATGGATATGTTGCTGGCTAAGAAACGCTCGCCCG
ATCGTAAAAACTGGCTCGAGGATAAGGGTGACATGGCGGTACTTTAA

Upstream 100 bases:

>100_bases
AGCGATATCTGCAAACTGTAAGCCAATTTTTACACTTACCTTGACAAATAGTCGTCCAGAGCCGCAACATGGCCGGATAT
AACAACTTATAGCGTACACT

Downstream 100 bases:

>100_bases
TGTTGTGCACTATTGCCGATAACAATAAAACTTAGGCATAAAGAAAATTACCATGTTGTATATAAAAACTAATAATTATA
AATATTTAAGGGTGTTCTCT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MTNQYTSDAIEVLNGLDPVKRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHATKIEVVLHTDNSLEVTDDGRGMPVDIHP
EEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSRRVEVTVRRDGQVYDIAFENGFKVEELKVTGTCGRRN
TGTRVHFWPDASYFDSANFSNSKLIYLLRAKAVLCPGLRIKFTNKHTNEVHEWYYESGLTDYLKAAVGDNLMLPEEPFIG
SFKGQIEAADWAITWLPEGGDAINESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLIPRGIKLSPEDIWDRAAFIL
SVKMQDPQFAGQTKEKLSSRQSSAFVSGIVRDAFSLWLNSNTELAESLADMCINNAQRRLKASKKVARKKVTSGPALPGK
LTDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSNDISEL
RYGKICILADADSDGLHIATLLCALFLKHYRVLVEQGHVYIAMPPLFRIDIGKDVYYALDEAEKEGILDRITAENKKGKV
QVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDVEETDSLMDMLLAKKRSPDRKNWLEDKGDMAVL

Sequences:

>Translated_628_residues
MTNQYTSDAIEVLNGLDPVKRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHATKIEVVLHTDNSLEVTDDGRGMPVDIHP
EEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSRRVEVTVRRDGQVYDIAFENGFKVEELKVTGTCGRRN
TGTRVHFWPDASYFDSANFSNSKLIYLLRAKAVLCPGLRIKFTNKHTNEVHEWYYESGLTDYLKAAVGDNLMLPEEPFIG
SFKGQIEAADWAITWLPEGGDAINESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLIPRGIKLSPEDIWDRAAFIL
SVKMQDPQFAGQTKEKLSSRQSSAFVSGIVRDAFSLWLNSNTELAESLADMCINNAQRRLKASKKVARKKVTSGPALPGK
LTDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSNDISEL
RYGKICILADADSDGLHIATLLCALFLKHYRVLVEQGHVYIAMPPLFRIDIGKDVYYALDEAEKEGILDRITAENKKGKV
QVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDVEETDSLMDMLLAKKRSPDRKNWLEDKGDMAVL
>Mature_627_residues
TNQYTSDAIEVLNGLDPVKRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHATKIEVVLHTDNSLEVTDDGRGMPVDIHPE
EGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSRRVEVTVRRDGQVYDIAFENGFKVEELKVTGTCGRRNT
GTRVHFWPDASYFDSANFSNSKLIYLLRAKAVLCPGLRIKFTNKHTNEVHEWYYESGLTDYLKAAVGDNLMLPEEPFIGS
FKGQIEAADWAITWLPEGGDAINESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNLIPRGIKLSPEDIWDRAAFILS
VKMQDPQFAGQTKEKLSSRQSSAFVSGIVRDAFSLWLNSNTELAESLADMCINNAQRRLKASKKVARKKVTSGPALPGKL
TDCSGQDPMRGELFLVEGDSAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSNDISELR
YGKICILADADSDGLHIATLLCALFLKHYRVLVEQGHVYIAMPPLFRIDIGKDVYYALDEAEKEGILDRITAENKKGKVQ
VTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDVEETDSLMDMLLAKKRSPDRKNWLEDKGDMAVL

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=620, Percent_Identity=25.3225806451613, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI19913406, Length=618, Percent_Identity=23.7864077669903, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=74.5222929936306, Blast_Score=1005, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=39.2792792792793, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=627, Percent_Identity=22.3285486443381, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=589, Percent_Identity=24.2784380305603, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=252, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=627, Percent_Identity=24.2424242424242, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=578, Percent_Identity=22.8373702422145, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69777; Mature: 69646

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQYTSDAIEVLNGLDPVKRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHATKIEVVLH
CCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCEEEEEEEE
TDNSLEVTDDGRGMPVDIHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
ALSRRVEVTVRRDGQVYDIAFENGFKVEELKVTGTCGRRNTGTRVHFWPDASYFDSANFS
HHHHEEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
NSKLIYLLRAKAVLCPGLRIKFTNKHTNEVHEWYYESGLTDYLKAAVGDNLMLPEEPFIG
CCCEEEEEEEHHHHCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
SFKGQIEAADWAITWLPEGGDAINESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNL
CCCCCEEECCEEEEEECCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IPRGIKLSPEDIWDRAAFILSVKMQDPQFAGQTKEKLSSRQSSAFVSGIVRDAFSLWLNS
CCCCCCCCHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTELAESLADMCINNAQRRLKASKKVARKKVTSGPALPGKLTDCSGQDPMRGELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
SAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSNDISEL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCHHHH
RYGKICILADADSDGLHIATLLCALFLKHYRVLVEQGHVYIAMPPLFRIDIGKDVYYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEHH
EAEKEGILDRITAENKKGKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDVEETD
HHHHCCHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCHHHHH
SLMDMLLAKKRSPDRKNWLEDKGDMAVL
HHHHHHHHHCCCCCHHHHHCCCCCEECC
>Mature Secondary Structure 
TNQYTSDAIEVLNGLDPVKRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHATKIEVVLH
CCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCEEEEEEEE
TDNSLEVTDDGRGMPVDIHPEEGIPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
ALSRRVEVTVRRDGQVYDIAFENGFKVEELKVTGTCGRRNTGTRVHFWPDASYFDSANFS
HHHHEEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
NSKLIYLLRAKAVLCPGLRIKFTNKHTNEVHEWYYESGLTDYLKAAVGDNLMLPEEPFIG
CCCEEEEEEEHHHHCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
SFKGQIEAADWAITWLPEGGDAINESYVNLIPTPLGGTHVNGFRQGLLESMREFCEFRNL
CCCCCEEECCEEEEEECCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IPRGIKLSPEDIWDRAAFILSVKMQDPQFAGQTKEKLSSRQSSAFVSGIVRDAFSLWLNS
CCCCCCCCHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTELAESLADMCINNAQRRLKASKKVARKKVTSGPALPGKLTDCSGQDPMRGELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
SAGGSAKQARDREFQAIMPLRGKILNTWEVDASQVLASQEVHDISVAIGCDPDSNDISEL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCHHHH
RYGKICILADADSDGLHIATLLCALFLKHYRVLVEQGHVYIAMPPLFRIDIGKDVYYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEHH
EAEKEGILDRITAENKKGKVQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDVEETD
HHHHCCHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCHHHHH
SLMDMLLAKKRSPDRKNWLEDKGDMAVL
HHHHHHHHHCCCCCHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]