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The map label for this gene is aceF [H]

Identifier: 117918884

GI number: 117918884

Start: 487935

End: 489941

Strand: Direct

Name: aceF [H]

Synonym: Shewana3_0427

Alternate gene names: 117918884

Gene position: 487935-489941 (Clockwise)

Preceding gene: 117918883

Following gene: 117918885

Centisome position: 9.81

GC content: 52.62

Gene sequence:

>2007_bases
ATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGATGAAGTACAGGTTATCGAAATCTGTGCTTCTGTGGG
CGATACCTTAGCAGCGGAAGAGTCGATTCTGACCGTTGAAAGCGACAAGGCGACCATGGACATTCCTGCGCCGTTCGCGG
GTGTGTTAGCCGAATTGAAAGTGGCCGTCGGTGACAAAGTCAGCGAAGGTACCTTAATTGCGATGATGCAAGCAGCGGGC
ACGGCTAATGTTCAAGCTGCACCGGTTGCTCAAGCTGCCGCTCCTGCGCCAGCACCAGCAGCACCTGCTCCAGTACAAGC
GGCTCCTGCCCCTGTGGCTGCCGCGCCAGCCACGGGTGCAACCCAAGTGGTTGAAGTCACTGTGCCGGATATCGGTGGTG
ACACAGATGTGTCTGTTATCGAAGTCTTAGTTGCCGCTGGCGATAAGATTGAGGTGGACGCTGGCCTTATCACGCTTGAA
ACCGACAAAGCGACCATGGACGTACCATCGCCATTCGCGGGTGTGGTAAAAGAAGTGAAAGTGGCTGTGGGTGACAAGGT
TTCTCAAGGCTCCTTAGTGATCATGCTCGAAGTGGGTGGCGCAGCTCCTGCTGCCGCTGCTCCACAAGCAAGCGCGTCAG
CTCCTGTCGCTCAAGCTGCACCTGCGGCGGCTGTTGCTCCAGTAGCTGCTGCACCAGTGGTTGCGGTTAAAGAAATCCAA
GTGCCAGATATTGGTGATGCCAGCAATGTCGATGTGATCGAAGTGCTAGTGTCTGTGGGTGATATGATCACTGCTGACCA
AGGTTTGATCACGTTAGAAACCGATAAAGCGACCATGGAAGTGCCTGCGCCATTCGCCGGTAAACTGTTGTCGTTAACCG
TTAAAGTCGGTGATAAGGTTTCTCAAGGTAGCGTGATTGCGACGATTGAGACTGTGACTGCTGGTGCAGCTCCTGCGCCT
CAAGCCGCAGCACCTGCACCTGTCGCCCAAGAAGCGGCACCAGCACCTGTTGCAGCCGCGCCGAGCCGTCCACCGGTTCC
ACATCACCCAAGTGCGGGTGCGCCAGTGGCAACCGGTGTGGTGCATGCGTCTCCTGCGGTTCGCCGTTTGGCCCGTGAGT
TTGGTGTGGATCTCACTCAAGTGACGGGTTCTGGCCGTAAAGGTCGCATCATGAAGGAAGACGTACAGGCGTATGTGAAG
TACGAACTGTCTCGTCCTAAGGCGACAGCAGCAACTTCAGTTGCAGCGGGTAACGGTGGTGGTCTGCAAGTGATTGCTGC
ACCGAAAGTGGATTTCAGCAAGTTTGGTGAAGTGGAAGAAATTCCATTAAGCCGTATCCAGAAGATCTCTGGTCCAAACC
TACACCGTAACTGGGTAACTATTCCTCACGTGACTCAGTTCGATGAAGCTGATATCACCGAAATGGAAGAGTTCCGTAAG
CAGCAGAACGACGCGGCGGCGAAGAAGAAAGCCGATTATAAGATCACTCCGCTGGTCTTTATGATGAAAGCTGTGGCGAA
AACGCTGCAACAATTCCCTGTGTTCAACTCAAGCTTAAGCAGCGACGGTGAATCACTGATCCAGAAGAAGTACTACCACA
TCGGTGTGGCGGTGGATACGCCAAACGGTCTAGTGGTTCCTGTGGTACGTGATGTGGATAAGAAAGGCATTATCGAGTTA
TCTCGTGAGTTGGCCGATATTTCTATCCGCGCCCGCGATGGTAAGCTGAAGTCTGCCGATATGCAGGGTAGCTGCTTCAC
TATTTCAAGCCTAGGTGGCATTGGTGGTACTGCATTTACCCCAATCGTTAACTACCCAGACGTGGCGATTTTAGGTGTGT
CTAAATCTGAAATTAAGCCTAAGTGGAATGGTAAAGAATTCGAGCCTAAATTGATGTTGCCACTGTCGCTATCATACGAT
CACCGCGTGATCGATGGTGCTATGGCTGCACGCTTTAGCGTGACCCTGTCAGGAATTCTGTCCGATATTCGTACTTTGAT
TCTGTAA

Upstream 100 bases:

>100_bases
TCGTAAAGAGCTGCCTGTTGATGTGTTAGCGAATGCCATCAAGGAATACGGCATCGACGCTGACAAGATCAATCCACAGT
ACGCGTAAGAGGCAATAAAA

Downstream 100 bases:

>100_bases
ACAAATAAGGCTGCTCAACTTGAGTGGCCTTTTGTTTATTGTGATCAGTATCAAACACAGGTTAAAATGCGCCCACCTTA
CGCGCTGGCGCATTTTCGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
TANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLE
TDKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAAPQASASAPVAQAAPAAAVAPVAAAPVVAVKEIQ
VPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAP
QAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVK
YELSRPKATAATSVAAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRK
QQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKKGIIEL
SRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYD
HRVIDGAMAARFSVTLSGILSDIRTLIL

Sequences:

>Translated_668_residues
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
TANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLE
TDKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAAPQASASAPVAQAAPAAAVAPVAAAPVVAVKEIQ
VPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAP
QAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVK
YELSRPKATAATSVAAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRK
QQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKKGIIEL
SRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYD
HRVIDGAMAARFSVTLSGILSDIRTLIL
>Mature_667_residues
AELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAGT
ANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGATQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLET
DKATMDVPSPFAGVVKEVKVAVGDKVSQGSLVIMLEVGGAAPAAAAPQASASAPVAQAAPAAAVAPVAAAPVVAVKEIQV
PDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAPQ
AAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGVVHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKY
ELSRPKATAATSVAAGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQ
QNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDTPNGLVVPVVRDVDKKGIIELS
RELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDH
RVIDGAMAARFSVTLSGILSDIRTLIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=28.110599078341, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI31711992, Length=342, Percent_Identity=32.4561403508772, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI203098816, Length=437, Percent_Identity=28.604118993135, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI203098753, Length=437, Percent_Identity=28.3752860411899, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786305, Length=664, Percent_Identity=55.1204819277108, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=29.5843520782396, Blast_Score=173, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=456, Percent_Identity=27.8508771929825, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.5068493150685, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=443, Percent_Identity=30.2483069977427, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=30.4487179487179, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=433, Percent_Identity=29.3302540415704, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=26.1574074074074, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=29.0909090909091, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.5140186915888, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24582497, Length=316, Percent_Identity=28.7974683544304, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=316, Percent_Identity=28.7974683544304, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68544; Mature: 68413

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGTANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGA
HHHCCCCCCCEEEEEEECCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLETDKATMDVPSPFAGVVKEVK
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
VAVGDKVSQGSLVIMLEVGGAAPAAAAPQASASAPVAQAAPAAAVAPVAAAPVVAVKEIQ
HHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHCCEEEEEEEC
VPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKV
CCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCC
SQGSVIATIETVTAGAAPAPQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGV
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCE
VHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVAAGNGG
EECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCC
GLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRK
CEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHH
QQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDT
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEEEEEEEEEC
PNGLVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFT
CCCEEEEHECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
PIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGIL
CCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SDIRTLIL
HHHHHHHC
>Mature Secondary Structure 
AELKEVFVPDIGGDEVQVIEICASVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGTANVQAAPVAQAAAPAPAPAAPAPVQAAPAPVAAAPATGA
HHHCCCCCCCEEEEEEECCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQVVEVTVPDIGGDTDVSVIEVLVAAGDKIEVDAGLITLETDKATMDVPSPFAGVVKEVK
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
VAVGDKVSQGSLVIMLEVGGAAPAAAAPQASASAPVAQAAPAAAVAPVAAAPVVAVKEIQ
HHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHCCEEEEEEEC
VPDIGDASNVDVIEVLVSVGDMITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKV
CCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCC
SQGSVIATIETVTAGAAPAPQAAAPAPVAQEAAPAPVAAAPSRPPVPHHPSAGAPVATGV
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCE
VHASPAVRRLAREFGVDLTQVTGSGRKGRIMKEDVQAYVKYELSRPKATAATSVAAGNGG
EECCHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCC
GLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRK
CEEEEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHH
QQNDAAAKKKADYKITPLVFMMKAVAKTLQQFPVFNSSLSSDGESLIQKKYYHIGVAVDT
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEEEEEEEEEC
PNGLVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFT
CCCEEEEHECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
PIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGIL
CCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SDIRTLIL
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]