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The map label for this gene is insF1 [C]

Identifier: 117918798

GI number: 117918798

Start: 376047

End: 376850

Strand: Direct

Name: insF1 [C]

Synonym: Shewana3_0341

Alternate gene names: 117918798

Gene position: 376047-376850 (Clockwise)

Preceding gene: 117918797

Following gene: 117918806

Centisome position: 7.56

GC content: 45.65

Gene sequence:

>804_bases
ATGTCGAGGCAAGGTATTTATCAGGCAGTTGCTCGAATGGAAAGTCGTAGAGCTGAGCTATCGGTTATCAAAGACTGGGT
CCAGTACTGGCGTAAATATATGCCAAGGTTAGGGGCCCGTAAGCTCTACACGTTGATAAAATCCAAGTTGGTTGAGCACA
ATATTAAACTCGGGCGAGATGGGTTCTTTACCTATTTGAGAAGTGAAGGTTTACTGGTTAAACCGAAGAAAAGCTACACA
AAAACCACGTTCAGCAAACACTGGATGAAGAAGCATCCTAATCTGCTGAAAGAGGAGGGACTGCACGATGCAGGGCATGT
ACTGGTCAGCGATATCACCTATCTTGAGTCAGACCAAGGTGTGCACTACCTGTCACTGGTCACCGATGCCAGTTCTCGTA
AAATAGTCGGTCATCACGTGAGTAAAGACATGAAAGCCGAGAGTGTGGTGAAAGCGTTAAAAATGGCCATCAAAGATAAG
CGCTATATCGGCAACGCGGTGCATCACTCAGACAGAGGCTTACAGTACTGCTCAGCCGTTTATCAGAATGCGCTTCAGGC
GAGCCAAATCCAGCCGTCAATGACGGATGGTTATGATTGCTACCAAAATGCATTAGCAGAAAGAGTAAATGGCATACTGA
AGCAGGAGTTTTTACTGCACCGATGTAGGACATTTGCGGAGCTGAAGATACTTGTTAGAGAATCGATAGCAATATACAAC
AATATGAGACCGCATCTGAGTTTGAATATGGACTCCCCTAATCAGGTGCACAATAGAAAAGGCCAGCTACTGGAGCTGGC
CTAA

Upstream 100 bases:

>100_bases
AATATGGAGCTGGCCTCAGAAAAAAGTACTTATCCGGTACGTCTGGCAAGCAAAAGGCAACGGAGAAATAAACCTCGCTG
CCGCATGTCGTGCTGCGGGT

Downstream 100 bases:

>100_bases
AAACTGTCAACCTATCTTAGGACGAGACAAACCTATGGCTTCATCCGGCAATTCGGGCTCTTTGAGCCGTGCGAGCCGTT
CAATCATGCTCGGGCCTTGG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHNIKLGRDGFFTYLRSEGLLVKPKKSYT
KTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDK
RYIGNAVHHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYN
NMRPHLSLNMDSPNQVHNRKGQLLELA

Sequences:

>Translated_267_residues
MSRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHNIKLGRDGFFTYLRSEGLLVKPKKSYT
KTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDK
RYIGNAVHHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYN
NMRPHLSLNMDSPNQVHNRKGQLLELA
>Mature_266_residues
SRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHNIKLGRDGFFTYLRSEGLLVKPKKSYTK
TTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDKR
YIGNAVHHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYNN
MRPHLSLNMDSPNQVHNRKGQLLELA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=190, Percent_Identity=33.1578947368421, Blast_Score=98, Evalue=6e-21,
Organism=Escherichia coli, GI1788405, Length=231, Percent_Identity=31.6017316017316, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787263, Length=231, Percent_Identity=31.6017316017316, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786753, Length=231, Percent_Identity=31.6017316017316, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786570, Length=231, Percent_Identity=31.6017316017316, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786490, Length=231, Percent_Identity=31.6017316017316, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789981, Length=152, Percent_Identity=32.8947368421053, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30788; Mature: 30657

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEECCCCCHHHHHCCCCEEECC
>Mature Secondary Structure 
SRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHNIKLGRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
GFFTYLRSEGLLVKPKKSYTKTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQG
HHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCC
VHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDKRYIGNAVHHSDRGLQYCSAV
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
YQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMRPHLSLNMDSPNQVHNRKGQLLELA
CCCCCEEECCCCCHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]