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The map label for this gene is epsE [H]

Identifier: 117918610

GI number: 117918610

Start: 177108

End: 178673

Strand: Direct

Name: epsE [H]

Synonym: Shewana3_0152

Alternate gene names: 117918610

Gene position: 177108-178673 (Clockwise)

Preceding gene: 117918609

Following gene: 117918611

Centisome position: 3.56

GC content: 52.49

Gene sequence:

>1566_bases
ATGAGTGAAATACAAGTAACCCAAGTCGAGGATTTAAGCCTAGTTTCTAACGAACTAGGGCTTGAAGCCGAAGGTGATGA
GGTATTTCGCTCCAGCAGCAAAGAGCGTTTACCCTTTGCCTTTGCCCATCGCCATGAGGTGATTTTAGCCTATGGCGATG
ATGGTGCCTTGAACCTGTTTTATACCGCCAAAACCCCATTAGCAGCCATGCTCGAAGCGCGCCGCTACTCGGGCGCGGAT
TTGCCGCTGGTCTTGCTCGAGCCGGGCAAGTTTGAGGCGAAATTAACCCAGGCCTATCAAGCCAACTCCTCCGAAGCGCA
GCAGCTGATGGAAGATATTGGCAACGAGATGGATTTATTCACCCTCGCCGAAGAGCTGCCGCAAACCGAAGATCTGCTCG
AAGGCGATGATGATGCGCCTATTATCAAGCTGATTAACGCCTTGTTATCTGAAGCGATTAAAGAAGAAGCCTCGGATATC
CATATCGAAACCTACGAGAAGCAGTTGGTCGTGCGTTTCCGTATCGACGGTGTGCTGAAGGAAGTGCTTAAACCGAATCG
TAAGCTGTCATCCCTTTTGGTGTCGCGGATTAAGGTCATGGCGCGTCTCGATATCGCCGAGAAACGTGTACCACAGGACG
GCCGTATTTCGCTGCGGATTGCGGGTCGTGCGGTGGACGTGCGGGTATCGACCATGCCATCGAGCCATGGCGAGCGTGTG
GTGCTGCGTCTGTTAGATAAAAACGCTGGCAATTTGGACTTAAAACAACTGGGGATGACCGACAGTATCCGTGCCCAGTT
TGACGAGATTATCCGTCGTCCACACGGCATTATTCTGGTGACAGGCCCTACGGGTTCGGGTAAGAGTACCACGCTGTACG
CGGGTCTTACCGAGATTAACTCTAAAGATACCAATATCTTAACCGTTGAAGACCCGATCGAATACGAGTTAGAAGGTATA
GGTCAAACTCAAGTGAACACTAAGGCGGACATGACCTTCGCCCGTGGTCTGCGTGCGATTCTGCGTCAAGACCCGGATGT
GGTGATGATCGGGGAAATCCGTGACCTAGAAACCGCCCAAATTGCGGTGCAGGCATCTTTGACTGGTCACTTAGTGATTT
CAACCCTGCATACCAACACGGCCTCTGGCGCGATTACCCGTTTGCAGGATATGGGCGTAGAGCCTTTCTTAGTCTCATCG
AGTTTGCTCGGGGTACTAGCCCAGCGCTTAATTCGTACTCTCTGTCCAAAATGTAAGACAGAGCATGTGCCCGATGAGCG
TGAGCGTGAGCTGCTGGGAATGGCAGCAGACGATCATCGCCATATTTACCGCGCCAATGGCTGTAAGGCCTGTGGTAGCA
GTGGCTACCGTGGTCGTACGGGTATCCATGAGCTGTTGCTGGTCGATGATAATGTGCGCGAGCTGATCCACGGTGGACGT
GGCGAACTGGCGATTGAGAAATATATCCGCCAGTCGGTGCCAAGCATTCGCCACGATGGCATGAGCAAGGTGCTCTCAGG
TATCACCACTCTAGAAGAAGTCCTGAGGGTGACCCGCGAGGAGTAA

Upstream 100 bases:

>100_bases
TAGATCGTTACAAAGAAGGTAAAGGGTTAGAAACTCAGATGCGTAAAACGGACTCGACCCTCAAGTCGCTGGATGAAAAC
AAGAACAAAGACAAAACACA

Downstream 100 bases:

>100_bases
GCTATGCCAGCATTTGAATATAAGGCGCTGGACGCCAAGGGAAAACAGCTAAAAGGGGTGATTGAGGCGGATACCGCCAG
ACATGCCCGTAGTCAGCTGC

Product: type II secretion system protein E (GspE)

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAYGDDGALNLFYTAKTPLAAMLEARRYSGAD
LPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLKQLGMTDSIRAQFDEIIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKTEHVPDERERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE

Sequences:

>Translated_521_residues
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAYGDDGALNLFYTAKTPLAAMLEARRYSGAD
LPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDI
HIETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLKQLGMTDSIRAQFDEIIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGI
GQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLIRTLCPKCKTEHVPDERERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
>Mature_520_residues
SEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAYGDDGALNLFYTAKTPLAAMLEARRYSGADL
PLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIH
IETYEKQLVVRFRIDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERVV
LRLLDKNAGNLDLKQLGMTDSIRAQFDEIIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEGIG
QTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSS
LLGVLAQRLIRTLCPKCKTEHVPDERERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGRG
ELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=57.4112734864301, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=43.75, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI87082188, Length=245, Percent_Identity=36.734693877551, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57584; Mature: 57452

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAYGDDGALNLF
CCCCEEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEE
YTAKTPLAAMLEARRYSGADLPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLKQLGMTDSIRAQFDEIIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELEGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKT
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDERERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIQVTQVEDLSLVSNELGLEAEGDEVFRSSSKERLPFAFAHRHEVILAYGDDGALNLF
CCCEEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEE
YTAKTPLAAMLEARRYSGADLPLVLLEPGKFEAKLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLVVRFRIDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLKQLGMTDSIRAQFDEIIRRPHGIILVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SKDTNILTVEDPIEYELEGIGQTQVNTKADMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHE
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPKCKT
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EHVPDERERELLGMAADDHRHIYRANGCKACGSSGYRGRTGIHELLLVDDNVRELIHGGR
CCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCC
GELAIEKYIRQSVPSIRHDGMSKVLSGITTLEEVLRVTREE
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]