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The map label for this gene is epsD [H]

Identifier: 117918609

GI number: 117918609

Start: 175003

End: 177111

Strand: Direct

Name: epsD [H]

Synonym: Shewana3_0151

Alternate gene names: 117918609

Gene position: 175003-177111 (Clockwise)

Preceding gene: 117918608

Following gene: 117918610

Centisome position: 3.52

GC content: 48.74

Gene sequence:

>2109_bases
ATGAATAACAAACGGATTCGACGCAAACTGATTGCTGGAGTTGTGGCAGGCGCCACTATGCTGACCTCGCAATTTGTTTG
GTCTGAACAATATGCGGCCAACTTTAAAGGCACTGATATCCAAGAATTTATCAACATTGTTGGTAAAAACCTGAATAAAA
CCATCATTGTTGACCCGACCATTCGCGGCAAGATCAACGTGCGCAGTTATGATCTGCTCAACGATGAGCAGTATTACCAA
TTCTTCCTCAACGTGTTGCAAGTCTACGGTTATGCCATCGTCGAGATGGAAAACAACGTCATCAAAGTCATTAAAGACAA
AGATGCGAAAACCGCGGCGATTCGTGTTGCTAACGACAACGACCCAGGTTTAGGTGATGAAATGGTCACCCGCATCGTGG
CGCTGTATAACACCGAAGCCAAGCAGTTAGCGCCGTTGTTACGTCAGTTAAACGATAACGCCGGTGGCGGTAACGTAGTG
AACTACGACCCATCGAACGTACTGATGCTGTCAGGCCGCGCCGCTGTAGTTAACAAGTTAGTCGAAATCGTTCGCCGTGT
AGATAAACAGGGTGATACCTCGGTACAAGTAGTGCCGCTCGAATACGCCTCAGCCGGTGAAATGGTGCGAATTATCGATA
CCCTTTACCGCGCGACAGCTAACCAAGCGCAGCTCCCAGGACAAGCGCCTAAGGTTGTTGCCGACGAGCGTATTAACGCC
GTCGTGGTCAGTGGTGATGAAAAGAGCCGCCAACGCGTGGTTGAGCTTATCCATCGCCTCGATGCCGAGCAGGCTAGCAC
GGGTAACACTAAGGTACGTTATTTACGTTATGCCAAGGCTGAAGACTTGGTTGAAGTGCTGACCGGATTTGCGCAAAAGC
TTGAGGGTGAAAAAGATCCAAGCGCCCAAGCTGGCGGTAAACGTCGTAATGAAATCAATATTATGGCCCACACTGATACC
AACGCCTTAGTGATCAGCGCCGAGCCAGATCAAATGCGCACAATCGAGAGCGTGATCAACCAACTCGATATTCGCCGCGC
TCAAGTATTAGTTGAAGCCATTATCGTGGAAGTTGCCGAAGGCGATAACGTCGGCTTTGGGGTGCAATGGGCCGCGAAAG
CGGATGGTGGCACTCAGTTCAATAACTTAGGCCCAACCATTGGTGAAATTGGCGCTGGTATTTGGCAGGCACAAGATAAA
GAAGGGACTTATATTACCAACCCTAGTACGGGGGAAGTGATCGGTAAAAACCCTGATACTAAAGGCGATGTTACTTTACT
CGCCCAAGCCTTAGGTAAAGTGAATGGTATGGCGTGGGGCGTGGCCATGGGCGACTTTGGCGCCCTAGTTCAAGCTGTGT
CGGCCGATACCAACTCAAACGTTCTGGCAACACCTTCGATTACGACATTGGATAACCAAGAAGCCTCGTTTATCGTCGGT
GACGAAGTGCCGATTCTGACGGGTTCTACCGCCAGCTCAAACAACAGTAACCCATTCCAAACCGTTGAACGGAAAGAGGT
TGGGGTGAAGTTAAAAGTGGTGCCTCAAATCAACGAAGGCAACGCGGTTAAGCTTGCGATTGAGCAAGAAGTCTCGGGCG
TAAACGGTAACACGGGTGTAGATATCTCCTTCGCGACTCGCCGTTTAACCACCACTGTAATGGCCGACTCAGGACAAATC
GTGGTGCTCGGTGGTTTGATTAACGAAGAAGTCCAAGAAAGCGTGCAGAAAGTGCCATTCCTCGGCGATATTCCGGTGCT
CGGACACTTATTCAAGTCTTCTTCCAGTAAGAAGACCAAAAAGAACCTGATGATCTTTATCAAGCCCACCATTATCCGTG
ATGGTGTGACTATGGAAGGTATCGCAGGCCGTAAATACAACTATTTCAGAGCATTACAACTTGAGCAGCAAGAGCGCGGC
GTGAACCTGATGCCAAATACTCAAGTGCCAATTCTGGAAGAATGGAATCAATCTGAATATCTGCCGCCCGAAGTGAACGA
CATTTTAGATCGTTACAAAGAAGGTAAAGGGTTAGAAACTCAGATGCGTAAAACGGACTCGACCCTCAAGTCGCTGGATG
AAAACAAGAACAAAGACAAAACACAATGA

Upstream 100 bases:

>100_bases
TGAATTAACTGAAGTTTCCATCATGGTTGAGCGGGAAGGACAATTGGTTGAAATCATGTTTAGTTTGCCGCAATAACACG
CGTTAGAGGAAAGTAAAATA

Downstream 100 bases:

>100_bases
GTGAAATACAAGTAACCCAAGTCGAGGATTTAAGCCTAGTTTCTAACGAACTAGGGCTTGAAGCCGAAGGTGATGAGGTA
TTTCGCTCCAGCAGCAAAGA

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDK
EGTYITNPSTGEVIGKNPDTKGDVTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVG
DEVPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQI
VVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERG
VNLMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLETQMRKTDSTLKSLDENKNKDKTQ

Sequences:

>Translated_702_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDK
EGTYITNPSTGEVIGKNPDTKGDVTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVG
DEVPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQI
VVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERG
VNLMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLETQMRKTDSTLKSLDENKNKDKTQ
>Mature_702_residues
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMLSGRAAVVNKLVEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAGGKRRNEINIMAHTDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVGFGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDK
EGTYITNPSTGEVIGKNPDTKGDVTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVG
DEVPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGVDISFATRRLTTTVMADSGQI
VVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERG
VNLMPNTQVPILEEWNQSEYLPPEVNDILDRYKEGKGLETQMRKTDSTLKSLDENKNKDKTQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=665, Percent_Identity=43.4586466165414, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1789793, Length=369, Percent_Identity=25.4742547425474, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 76692; Mature: 76692

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
IRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDN
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
DPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHH
VEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCE
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDP
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
SAQAGGKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDNVGFGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDKEGTYITNPSTGEVIGKNPDT
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCEECCCCCC
KGDVTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVG
CCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEEC
DEVPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGV
CCCEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCE
DISFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTK
EEEEHHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
KNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVNLMPNTQVPILEEWNQSEY
CCEEEEEECEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCC
LPPEVNDILDRYKEGKGLETQMRKTDSTLKSLDENKNKDKTQ
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNKRIRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
IRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDN
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
DPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHH
VEIVRRVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINA
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCE
VVVSGDEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDP
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
SAQAGGKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDNVGFGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDKEGTYITNPSTGEVIGKNPDT
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCEECCCCCC
KGDVTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVG
CCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEEC
DEVPILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNGNTGV
CCCEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCE
DISFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKTK
EEEEHHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
KNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQERGVNLMPNTQVPILEEWNQSEY
CCEEEEEECEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCC
LPPEVNDILDRYKEGKGLETQMRKTDSTLKSLDENKNKDKTQ
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]