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The map label for this gene is selB [H]

Identifier: 117918565

GI number: 117918565

Start: 122540

End: 124561

Strand: Direct

Name: selB [H]

Synonym: Shewana3_0107

Alternate gene names: 117918565

Gene position: 122540-124561 (Clockwise)

Preceding gene: 117918564

Following gene: 117918566

Centisome position: 2.46

GC content: 54.8

Gene sequence:

>2022_bases
ATGATTATGGTCACCGCGGGCCATGTCGACCACGGCAAGAGCACGTTGATCCGTGCCTTAACGGGGATGAACACCGACAG
GCTCCCCGAAGAAAAACGCCGTGGCATGACCATCGATCTCGGTTACGCCTTTATGCCACTGCGGGATGGCACTCGCTTAG
CCTTTATTGATGTGCCCGGCCATGAAAAATTTATCAATAATATGCTGGTAGGCGTGAGCCACGTGCGCCACGCGCTGCTG
GTGTTGGCCTGTGACGATGGCGTGATGCCACAAACCCGCGAGCATCTGCAAATCCTCGCGCTTTTACCGCTAAACAGTTT
GACCTTAGTGCTGACCAAGCGGGATTTGGTCGATGACCAAACCGCCGCCAAGTTGACCCTAGAGGGAATGGCGCTGCTTG
CTGAATACGGTATCGAGGCCAGTGGCGTATTTGAGGTTTGCGCTAATGATGCAGGCGATGCGGGGGTAGATACCCTTAAG
CAACATATTTTGGATATTGCCGAGTTGCAATCGACCCAAGCGGAAGATGCCAGCAGCTTTAGGATGACGCTCGATCGCGC
CTTTAGCGTTAAAGGCGCCGGCTGTGTGGTCACGGGCACTGTGATTAGCGGCAGTGTGTGTATCGGCGATAGCCTCTACA
GCTCTGGGCAAAAAGCGAAATTACGGGTGCGCGGTATTCATTGCCAAGGGATGGAAGTCCACAGAGCCTTGAGTGGCACT
CGGGTCGCCTTAAATCTTACTGGCGTTGATAACCACAGGGCGCCCGCCCGCGGTGACTGGTTAAGCAGCCTGCCTCAGGC
GGAGCTGTGCGCCAGACCTGTAGTGCAAATTCGCAGTTTCGAACCCTTAACCCATTGGCAAAATGTGCATTGTCACCACG
GCGCCGACCATACCCTAGGGCGAGTATCGCTTCTGGATGAAGCCGATGAGCAGGGGCGTTATCTGGCCGAAATTGTGCTC
GATAAGCCATTGCTGCTATGTCAGGACGACCCGATAGTGCTCAGGCATATTGGTGGCAAGCAAACGCTGGGCGCTGGGCG
GGTGCTGGCATTAAAAGTTCCCAATCGTAAGAAGCGTACGCCGGAGCGTGTCAGTAAGTTGCAACAGCTGGCTCAGCTCT
CCAATCCGGTGAATGTGCTGGCGCTACTCGGTCAGACTGAGGCGCTCAGTATTGATGAAATCCGTTGGCGTTGGCAGCTA
ACCGATGAAGGGCTGGCTGCCATGCTGGCGCAGGCGGGGCTGACTCGCCTCGGAGCCTATGGGCTATCGACCCAGTTACT
TGAGCAAGAAAAACAGCAGTTTATCGATCTGTTACGTGAATACCACCAACAGCACCCCGACCAATTGGGGCTGGGGCGTA
ATCGCCTGCAACGCATGAGCCACAACGTATTGCCTAGCGAGCTTGCCAATAAGGTGCTCGATAGTCTCTGCCAAGAAGGG
CAGATGGTACTGCGCGGCGCCTTGCTGCAGCTGGCGGATCACAGGATTGTCCTCGATAGCGAGGCGCAGCAATGCTGGCA
ACGCTTGGCCCCTTGGTTGGCGCAGCAAACCTCGCCCGTGTGGGTGACTGAGATGGGGGAATATCTCGAACTCGAGCCCG
CCAAACTCAGATTGCTATGTTTTCAACTGGTGCAACAGGGCTTTATTACCGCCGTAGTGAAGGATAGATATCTGCTGAGC
GAGCAGTTGTGTCATTACGCCAATCTGGTGCGAGCGCATGTGGAAACCCACGGCAAGCTCGAAACCGCAGAGTTTAAGGA
TCTGATTGGCCTAGGCCGCAAGGTCAGTATTCAGCTGTTGGAGTTTTTTGACCGCAGTGGTTTTACCCGTAGGAAGTATC
GCAGTAACAGTCGGGAGATTCGGGATGGGGAACTGTTTTATTCCCATGAGAATTGGCGGAAGAACATAGGAGTCGAACCT
ACCCAGGACTGCTGGCAGCCCTACCCGGATTTGAAGTCCGGACGCCTCACCGGAGACGACGTTCTTCCAATAGAAGACAA
ACCGATAGTAGAAAATGCATAA

Upstream 100 bases:

>100_bases
TGATTGGCCGTATGACCCAAGATCAACTTCGCCTCGATCTGCGCGGTATCGATGATGAGGCCGAGTTGTTAGCCGAACTC
AGCACCTTAGGTGTGCAGCT

Downstream 100 bases:

>100_bases
ACCTGAATCAAGTGCATTCGTATTTAGTTAGCCACTTTGTGACTTGAGTCGAACTTTATTGGCAAACCAATTGTTTTTAT
TTGAATTTATGATTTTAGAG

Product: selenocysteine-specific translation elongation factor SelB

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTLEGMALLAEYGIEASGVFEVCANDAGDAGVDTLK
QHILDIAELQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAQLSNPVNVLALLGQTEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIDLLREYHQQHPDQLGLGRNRLQRMSHNVLPSELANKVLDSLCQEG
QMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVRAHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPIEDKPIVENA

Sequences:

>Translated_673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTLEGMALLAEYGIEASGVFEVCANDAGDAGVDTLK
QHILDIAELQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAQLSNPVNVLALLGQTEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIDLLREYHQQHPDQLGLGRNRLQRMSHNVLPSELANKVLDSLCQEG
QMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVRAHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPIEDKPIVENA
>Mature_673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTLEGMALLAEYGIEASGVFEVCANDAGDAGVDTLK
QHILDIAELQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAQLSNPVNVLALLGQTEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIDLLREYHQQHPDQLGLGRNRLQRMSHNVLPSELANKVLDSLCQEG
QMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVRAHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPIEDKPIVENA

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI54607086, Length=358, Percent_Identity=25.6983240223464, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI34147630, Length=260, Percent_Identity=31.9230769230769, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI53729339, Length=284, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=284, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI2367247, Length=626, Percent_Identity=42.1725239616613, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1790412, Length=275, Percent_Identity=30.9090909090909, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789737, Length=275, Percent_Identity=30.9090909090909, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789108, Length=146, Percent_Identity=30.8219178082192, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1789559, Length=353, Percent_Identity=28.8951841359773, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=372, Percent_Identity=26.8817204301075, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17506081, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=279, Percent_Identity=30.1075268817204, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=271, Percent_Identity=31.7343173431734, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921738, Length=271, Percent_Identity=31.7343173431734, Blast_Score=122, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281363316, Length=227, Percent_Identity=30.3964757709251, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17864358, Length=227, Percent_Identity=30.3964757709251, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922690, Length=324, Percent_Identity=28.0864197530864, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550900, Length=299, Percent_Identity=25.4180602006689, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28572034, Length=97, Percent_Identity=37.1134020618557, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 75087; Mature: 75087

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00039 DEAD_ATP_HELICASE ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHVRHALLVLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQ
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHEEEEEECCCCEEEEEEECHHCCCC
TAAKLTLEGMALLAEYGIEASGVFEVCANDAGDAGVDTLKQHILDIAELQSTQAEDASSF
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
RMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
HHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEEECCHHHHHHCCCC
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLG
EEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
RVSLLDEADEQGRYLAEIVLDKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRT
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
PERVSKLQQLAQLSNPVNVLALLGQTEALSIDEIRWRWQLTDEGLAAMLAQAGLTRLGAY
HHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHC
GLSTQLLEQEKQQFIDLLREYHQQHPDQLGLGRNRLQRMSHNVLPSELANKVLDSLCQEG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
QMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLC
HHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCEEHCCCCCHHCCCHHHHHHHH
FQLVQQGFITAVVKDRYLLSEQLCHYANLVRAHVETHGKLETAEFKDLIGLGRKVSIQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHH
EFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEPTQDCWQPYPDLKSGRLTGDD
HHHHCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
VLPIEDKPIVENA
CCCCCCCCCCCCC
>Mature Secondary Structure
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHVRHALLVLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQ
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHEEEEEECCCCEEEEEEECHHCCCC
TAAKLTLEGMALLAEYGIEASGVFEVCANDAGDAGVDTLKQHILDIAELQSTQAEDASSF
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
RMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
HHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEEECCHHHHHHCCCC
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLG
EEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
RVSLLDEADEQGRYLAEIVLDKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRT
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
PERVSKLQQLAQLSNPVNVLALLGQTEALSIDEIRWRWQLTDEGLAAMLAQAGLTRLGAY
HHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHC
GLSTQLLEQEKQQFIDLLREYHQQHPDQLGLGRNRLQRMSHNVLPSELANKVLDSLCQEG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
QMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLAQQTSPVWVTEMGEYLELEPAKLRLLC
HHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCEEHCCCCCHHCCCHHHHHHHH
FQLVQQGFITAVVKDRYLLSEQLCHYANLVRAHVETHGKLETAEFKDLIGLGRKVSIQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHH
EFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEPTQDCWQPYPDLKSGRLTGDD
HHHHCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
VLPIEDKPIVENA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]