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The map label for this gene is merA [H]

Identifier: 117676250

GI number: 117676250

Start: 200669

End: 202324

Strand: Reverse

Name: merA [H]

Synonym: Shewana3_4311

Alternate gene names: 117676250

Gene position: 202324-200669 (Counterclockwise)

Preceding gene: 117676251

Following gene: 117676249

Centisome position: 72.53

GC content: 51.93

Gene sequence:

>1656_bases
ATGATCTTACTATCGATAGAAGGGATGACCTGTCCAAGTTGCGTCGCTCACGTTAAAGAAGCGCTCGATGCGATCGAAGG
CGTGAATAAGGTTGAAATATCTTATGAAAACGCCAGCGCAACGATCACCACAAACGGTGGGGTCAGCGTAACCGATCTCA
TTGGAGCGATTGAAGCCCTGGGTTATAACCCCTTAGCGGAAAACACAGCACCGAACGCCTACTGTGACAACGAAAATACC
AGCAACACAGAAAGCAACCGAACTCAACATGTGGCGATTATCGGTACGGGTTCCGGCGCTTTTGCCTGTGCTATCAAAGC
CGCTGAAGGTGGTGCCAAGGTCACTCTTATTGAGGGAGCCGATGTGATTGGCGGCTGTTGCGTGAATGTCGGCTGCGTAC
CCTCAAAAATTTTGATCCGCGCCGCTCAATTGGCACAGCAGCAACGCAATAATCCCTTTGCCGGACTGGAAAATCATGCG
CCCCAATTGAGTCGAGCCTTGTTGGCTCAGCAGCAGACCGCTCGAGTCGAAGAGCTGCGCGCTGCAAAGTATCAGAATAT
TCTAGAGACAAATCCTGCGCTTAGTTTACTTAAAGGTTGGGCGCAATTTAAAAATGCAAACACCCTGATAGTCAGAAAAA
ATGATGGCACTGAGCAGGAAATCTTTGCTGATAAAATCCTGATCGCCACTGGCTCCACGCCAACCATTCCTCCCATCGAG
GGGCTGGCCGAAACGCCTTACTGGACCTCTACCGAAGCGCTGTTTGCCGAGGAATTGCCGCAGCACCTGGTGGTGATTGG
TTCATCGGTCGTGGCGCTAGAAATTGCCCAGGCTTACCGACGATTGGGCAGTGAAGTCACCGTGTTGGCAAGACACTCAC
TACTTTACAGTGAAGACCCCATAATCGGTGAAAAACTGGCAGGCTGTTTTGAAAAAGAAGGCATTCGAGTTTTAAACAAC
ACGCAAGCCACCCAAGTGACCCACGATGGAAATCAATTCACCCTGAACACTAATGCAGGCGAGCTCAGTTGTGATCGTTT
GCTGGTAAGCACCGGGCGACACGCCAATACCAGCCAACTCAATCTGGACGCGGTGGGTGTCACCACCAACAAAAAGGGCG
AAATCGTTGTTAACGAACGCATGGAAACCAATGTTGCTGGTATTTACGCTGCCGGTGACTGCTCTAACATGCCGCAATTT
GTCTATGTCGCCGCGGCCGCCGGTAGCCGTGCTGGTATCAATATGACGGGCGGCGACGCCAAACTGGATCTGTCCACCAT
GCCCGCCGTGATCTTTACCGATCCACAGGTGGCTACCGTCGGACTGACCGAGGAACAAGCCAGGGCTCAGGATATTGAGA
CCGACAGCCGCGTACTGGGTATGGAAAATGTCCCACGTGCGTTAGCCAACTTTGAAACCGACGGTTTTATCAAACTGGTC
ACAGAGAAGGAAACGGGGTTGTTGCTAGGCGCGCAGATCCTGGCTCACGAAGGCGGCGAACTGATCCAGAGTGCGGCTTT
AGCCATCCGCAACCGTATGACGGTGACGGAATTAGCCGACCAGCTTTTCCCTTACCTCACCATGGTGGAAGGTTTGAAGC
TCTGCGCGCAAACCTTTAACAAGGATGTCAAAGAACTGTCCTGTTGTGCAGGATAA

Upstream 100 bases:

>100_bases
TCGCTTTGGGATATTTTTTCACCCGCGAATAAACGCTGTGATGATGACAGCTGTGCTGTTTAACACGACGCAATGCCTGA
CTACTATTTGAGGAAAACGA

Downstream 100 bases:

>100_bases
GAGAGGGCAGTATGGGAACGGCACCCGCCACGCATTCACTGACAGCCCCAACGGTCAATGCGTTGGCGGATGGATTGAAC
AAGGGTGAATTCGTTCCTTT

Product: putative mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MILLSIEGMTCPSCVAHVKEALDAIEGVNKVEISYENASATITTNGGVSVTDLIGAIEALGYNPLAENTAPNAYCDNENT
SNTESNRTQHVAIIGTGSGAFACAIKAAEGGAKVTLIEGADVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFAGLENHA
PQLSRALLAQQQTARVEELRAAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQEIFADKILIATGSTPTIPPIE
GLAETPYWTSTEALFAEELPQHLVVIGSSVVALEIAQAYRRLGSEVTVLARHSLLYSEDPIIGEKLAGCFEKEGIRVLNN
TQATQVTHDGNQFTLNTNAGELSCDRLLVSTGRHANTSQLNLDAVGVTTNKKGEIVVNERMETNVAGIYAAGDCSNMPQF
VYVAAAAGSRAGINMTGGDAKLDLSTMPAVIFTDPQVATVGLTEEQARAQDIETDSRVLGMENVPRALANFETDGFIKLV
TEKETGLLLGAQILAHEGGELIQSAALAIRNRMTVTELADQLFPYLTMVEGLKLCAQTFNKDVKELSCCAG

Sequences:

>Translated_551_residues
MILLSIEGMTCPSCVAHVKEALDAIEGVNKVEISYENASATITTNGGVSVTDLIGAIEALGYNPLAENTAPNAYCDNENT
SNTESNRTQHVAIIGTGSGAFACAIKAAEGGAKVTLIEGADVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFAGLENHA
PQLSRALLAQQQTARVEELRAAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQEIFADKILIATGSTPTIPPIE
GLAETPYWTSTEALFAEELPQHLVVIGSSVVALEIAQAYRRLGSEVTVLARHSLLYSEDPIIGEKLAGCFEKEGIRVLNN
TQATQVTHDGNQFTLNTNAGELSCDRLLVSTGRHANTSQLNLDAVGVTTNKKGEIVVNERMETNVAGIYAAGDCSNMPQF
VYVAAAAGSRAGINMTGGDAKLDLSTMPAVIFTDPQVATVGLTEEQARAQDIETDSRVLGMENVPRALANFETDGFIKLV
TEKETGLLLGAQILAHEGGELIQSAALAIRNRMTVTELADQLFPYLTMVEGLKLCAQTFNKDVKELSCCAG
>Mature_551_residues
MILLSIEGMTCPSCVAHVKEALDAIEGVNKVEISYENASATITTNGGVSVTDLIGAIEALGYNPLAENTAPNAYCDNENT
SNTESNRTQHVAIIGTGSGAFACAIKAAEGGAKVTLIEGADVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFAGLENHA
PQLSRALLAQQQTARVEELRAAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQEIFADKILIATGSTPTIPPIE
GLAETPYWTSTEALFAEELPQHLVVIGSSVVALEIAQAYRRLGSEVTVLARHSLLYSEDPIIGEKLAGCFEKEGIRVLNN
TQATQVTHDGNQFTLNTNAGELSCDRLLVSTGRHANTSQLNLDAVGVTTNKKGEIVVNERMETNVAGIYAAGDCSNMPQF
VYVAAAAGSRAGINMTGGDAKLDLSTMPAVIFTDPQVATVGLTEEQARAQDIETDSRVLGMENVPRALANFETDGFIKLV
TEKETGLLLGAQILAHEGGELIQSAALAIRNRMTVTELADQLFPYLTMVEGLKLCAQTFNKDVKELSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=31.7596566523605, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI50301238, Length=442, Percent_Identity=30.5429864253394, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI22035672, Length=449, Percent_Identity=29.6213808463252, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=29.1079812206573, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=29.1079812206573, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=29.1079812206573, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=29.1079812206573, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=29.1079812206573, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.9736842105263, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI291045268, Length=329, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=30.7522123893805, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789915, Length=352, Percent_Identity=36.0795454545455, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=27.6729559748428, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=26.1904761904762, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789765, Length=211, Percent_Identity=28.436018957346, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=30.3879310344828, Blast_Score=212, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI71983419, Length=350, Percent_Identity=31.4285714285714, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=350, Percent_Identity=31.4285714285714, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=454, Percent_Identity=25.7709251101322, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=423, Percent_Identity=23.1678486997636, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=28.1115879828326, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=457, Percent_Identity=29.5404814004376, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=26.215644820296, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358499, Length=494, Percent_Identity=31.9838056680162, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=27.7777777777778, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640553, Length=505, Percent_Identity=27.1287128712871, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=27.7777777777778, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17737741, Length=497, Percent_Identity=26.3581488933602, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281359715, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI281359713, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24639250, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI18543267, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24639252, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24639257, Length=222, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 58535; Mature: 58535

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLSIEGMTCPSCVAHVKEALDAIEGVNKVEISYENASATITTNGGVSVTDLIGAIEAL
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHH
GYNPLAENTAPNAYCDNENTSNTESNRTQHVAIIGTGSGAFACAIKAAEGGAKVTLIEGA
CCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEEECC
DVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFAGLENHAPQLSRALLAQQQTARVEELR
CCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQEIFADKILIATGSTPTIPPIE
HHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCEEEEEECCCCCCCCCC
GLAETPYWTSTEALFAEELPQHLVVIGSSVVALEIAQAYRRLGSEVTVLARHSLLYSEDP
CCCCCCCCCCHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCC
IIGEKLAGCFEKEGIRVLNNTQATQVTHDGNQFTLNTNAGELSCDRLLVSTGRHANTSQL
CCHHHHHHHHHHCCEEEECCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCCCEE
NLDAVGVTTNKKGEIVVNERMETNVAGIYAAGDCSNMPQFVYVAAAAGSRAGINMTGGDA
EEEEEEEECCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCEECCCCC
KLDLSTMPAVIFTDPQVATVGLTEEQARAQDIETDSRVLGMENVPRALANFETDGFIKLV
EEEHHHCCEEEEECCCEEEECCCHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCEEEEE
TEKETGLLLGAQILAHEGGELIQSAALAIRNRMTVTELADQLFPYLTMVEGLKLCAQTFN
EECCCCEEEHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDVKELSCCAG
HHHHHHHHCCC
>Mature Secondary Structure
MILLSIEGMTCPSCVAHVKEALDAIEGVNKVEISYENASATITTNGGVSVTDLIGAIEAL
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHH
GYNPLAENTAPNAYCDNENTSNTESNRTQHVAIIGTGSGAFACAIKAAEGGAKVTLIEGA
CCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEEECC
DVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFAGLENHAPQLSRALLAQQQTARVEELR
CCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQEIFADKILIATGSTPTIPPIE
HHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCEEEEEECCCCCCCCCC
GLAETPYWTSTEALFAEELPQHLVVIGSSVVALEIAQAYRRLGSEVTVLARHSLLYSEDP
CCCCCCCCCCHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCC
IIGEKLAGCFEKEGIRVLNNTQATQVTHDGNQFTLNTNAGELSCDRLLVSTGRHANTSQL
CCHHHHHHHHHHCCEEEECCCCEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCCCEE
NLDAVGVTTNKKGEIVVNERMETNVAGIYAAGDCSNMPQFVYVAAAAGSRAGINMTGGDA
EEEEEEEECCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCEECCCCC
KLDLSTMPAVIFTDPQVATVGLTEEQARAQDIETDSRVLGMENVPRALANFETDGFIKLV
EEEHHHCCEEEEECCCEEEECCCHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCEEEEE
TEKETGLLLGAQILAHEGGELIQSAALAIRNRMTVTELADQLFPYLTMVEGLKLCAQTFN
EECCCCEEEHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDVKELSCCAG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9167257 [H]