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The map label for this gene is ogt [H]

Identifier: 116872397

GI number: 116872397

Start: 1001464

End: 1001943

Strand: Direct

Name: ogt [H]

Synonym: lwe0979

Alternate gene names: 116872397

Gene position: 1001464-1001943 (Clockwise)

Preceding gene: 116872394

Following gene: 116872400

Centisome position: 35.59

GC content: 39.79

Gene sequence:

>480_bases
ATGCTACGGCAAAAAAACGAATCGCCAGTTGGAGAACTTTTTATTACGGTGGAAGATAGTTACCTTACTTCTATTGCTTA
CGATGAACCGACTCATTGGGAATATTCGGTAGGGGAAGAGAATGAACAGGATGTCTACAAACAAGTGATAAAACAAATGG
AAGCTTATTTTGAGGGTAAACTTGAATGTTTTGATGTTCCATTGCTCTCTCGAGGAACTGATTTTCAAAAACGTGTTTGG
CAAGCGTTAAGTGAAATTCCGTACGGAGCAGTAGTAAGTTATAAGGAAATTGCTATTAGAGCGGGGAGTCCAAAAGCAGT
CCAAGCGGTAGGGCAAGCAAATCGGGCGAACCCTTTCCCAATTATTATTCCTTGCCACAGATGTGTGAAAAGTAACGGAG
AGCTTGGTGGTTATAACGGAGCAGATGTAGATAAAAAACAATATTTGCTTGCATTAGAAAAAGGCTTGAGTTTTGATTAA

Upstream 100 bases:

>100_bases
TAGTAACAAAAAGTATCAATTATTAAAGGTAGATGACAGCCATTTGCGATTTTTGGATGGACATGCTATCTTTAGACAAT
CAACTATACGGAGGCAAATT

Downstream 100 bases:

>100_bases
CTCAAGCCTTTTTATTAAGCAGTTACTTGTTCAGTTATTACAATTTCTTTGTCATCATTTAAAGTTGCCAATAAGTTTTT
TGCATCAGGGTGATCAATGA

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MLRQKNESPVGELFITVEDSYLTSIAYDEPTHWEYSVGEENEQDVYKQVIKQMEAYFEGKLECFDVPLLSRGTDFQKRVW
QALSEIPYGAVVSYKEIAIRAGSPKAVQAVGQANRANPFPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSFD

Sequences:

>Translated_159_residues
MLRQKNESPVGELFITVEDSYLTSIAYDEPTHWEYSVGEENEQDVYKQVIKQMEAYFEGKLECFDVPLLSRGTDFQKRVW
QALSEIPYGAVVSYKEIAIRAGSPKAVQAVGQANRANPFPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSFD
>Mature_159_residues
MLRQKNESPVGELFITVEDSYLTSIAYDEPTHWEYSVGEENEQDVYKQVIKQMEAYFEGKLECFDVPLLSRGTDFQKRVW
QALSEIPYGAVVSYKEIAIRAGSPKAVQAVGQANRANPFPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=113, Percent_Identity=42.4778761061947, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787596, Length=164, Percent_Identity=32.3170731707317, Blast_Score=94, Evalue=3e-21,
Organism=Escherichia coli, GI1788542, Length=105, Percent_Identity=43.8095238095238, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=87, Percent_Identity=39.0804597701149, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=87, Percent_Identity=39.0804597701149, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=118, Percent_Identity=38.135593220339, Blast_Score=81, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17137554, Length=93, Percent_Identity=43.010752688172, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17885; Mature: 17885

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQKNESPVGELFITVEDSYLTSIAYDEPTHWEYSVGEENEQDVYKQVIKQMEAYFEGK
CCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LECFDVPLLSRGTDFQKRVWQALSEIPYGAVVSYKEIAIRAGSPKAVQAVGQANRANPFP
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHEEEECCCCHHHHHHHCCCCCCCCE
IIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSFD
EEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRQKNESPVGELFITVEDSYLTSIAYDEPTHWEYSVGEENEQDVYKQVIKQMEAYFEGK
CCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LECFDVPLLSRGTDFQKRVWQALSEIPYGAVVSYKEIAIRAGSPKAVQAVGQANRANPFP
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHEEEECCCCHHHHHHHCCCCCCCCE
IIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSFD
EEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA