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The map label for this gene is ilvE [H]

Identifier: 116872379

GI number: 116872379

Start: 984191

End: 985210

Strand: Direct

Name: ilvE [H]

Synonym: lwe0961

Alternate gene names: 116872379

Gene position: 984191-985210 (Clockwise)

Preceding gene: 116872376

Following gene: 116872380

Centisome position: 34.97

GC content: 38.53

Gene sequence:

>1020_bases
ATGAGTAAAGATATTGACTGGAGTAATTTAGGTTTTAGTTATATTAAAACGGACAAACGTTACATTTCTTATTGGAAAGA
CGGGGAATGGGACGAAGGTACACTAACGGAAGACAATACGCTACATATTAGCGAAGGCTCCACAGCGCTTCATTACGGAC
AACAATGTTTTGAAGGTTTGAAAGCATATCGATGTAAGGACGGTTCGATAAATCTTTTTCGCCCAGATCGTAATGCTGCT
CGAATTCAAAAAAGTTGTGAGCGTTTATTAATGCCGCATATCCCAACAGAAAAATTTATTGATGCTGTTATGCAGGTTGT
GCGTGCGAATGAAGAATTTGTTCCTCCATATGGCACAGGTGCAACACTTTATCTACGTCCATTTGTTATTGGTGTTGGTG
ATAATATTGGTGTACATGCAGCTCCAGAGTATATTTTCTCTGTTTTCTGCTCTCCAGTAGGACCATATTTCAAAGGTGGA
ATGGCGCCAACTAACTTTATCGTATCTGATTTTGACCGTGCTGCTCCAAATGGTACAGGTGCAGCAAAAGTAGGCGGAAA
CTATGCAGCAAGTTTACTTCCAGGCCAAGCAGCAAAAGAACGTAATTTTGGTGATTGTATTTATTTAGATCCAGCTACGC
ATACAAAAATTGAAGAAGTTGGTTCTGCTAATTTCTTTGGTATTACTAATGATGATAAATTCATTACACCATATTCACCT
TCTATTTTGCCAAGTATCACGAAATATTCATTGCTTTATTTGGCAGAACATCGTCTAGGTTTAAAAGCTGAAGAAGGCGA
TGTATATATTGATAAATTAGATGAATTCAAAGAAGCTGGAGCATGTGGAACAGCGGCGGTTATTACACCTATTGGTGGCA
TCCAAACGAAAGATGACTTCCATGTATTCCACAGCGAAACGGAAGTAGGACCTGTAACTAAACGCTTATTCGATGAACTG
TGTGGTATTCAATTTGGAGATATAGAGGCTCCAGAAGGTTGGATTTATAAAGTGAAATAA

Upstream 100 bases:

>100_bases
ATTTTCTTTTTCACATGACTTCACTGCAATAATGCTAGTTATTATGCTATACTGAAACATACAATAATTCTGTTTTAACG
ATAGAAAGTGGGGATTTTGA

Downstream 100 bases:

>100_bases
TGATAAAACCGTCCGTTCAGCGGGCGGTTTTTTATAATTAAAAGTAAAAGGCATTTCCCGCAAATTAAAAGGAAGTGCTT
TTTTTATTGACATAAATGCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAA
RIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGG
MAPTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITNDDKFITPYSP
SILPSITKYSLLYLAEHRLGLKAEEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDEL
CGIQFGDIEAPEGWIYKVK

Sequences:

>Translated_339_residues
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAA
RIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGG
MAPTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITNDDKFITPYSP
SILPSITKYSLLYLAEHRLGLKAEEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDEL
CGIQFGDIEAPEGWIYKVK
>Mature_338_residues
SKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAAR
IQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGM
APTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITNDDKFITPYSPS
ILPSITKYSLLYLAEHRLGLKAEEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDELC
GIQFGDIEAPEGWIYKVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=341, Percent_Identity=33.1378299120235, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI296010904, Length=341, Percent_Identity=33.1378299120235, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI296010906, Length=341, Percent_Identity=33.1378299120235, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI50658084, Length=344, Percent_Identity=30.8139534883721, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI258614015, Length=299, Percent_Identity=31.438127090301, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI296010902, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI296010900, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI48994963, Length=330, Percent_Identity=30.9090909090909, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17568601, Length=358, Percent_Identity=30.7262569832402, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17565728, Length=353, Percent_Identity=27.7620396600567, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322002, Length=340, Percent_Identity=32.3529411764706, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322608, Length=346, Percent_Identity=31.5028901734104, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=30.4733727810651, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37450; Mature: 37318

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGL
CCCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDRNAARIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTG
HHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGMAPTNFIVSDFDRAAPNGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCC
AAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITNDDKFITPYSP
CEECCCCEEECCCCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCCCC
SILPSITKYSLLYLAEHRLGLKAEEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDF
CHHHHHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
HVFHSETEVGPVTKRLFDELCGIQFGDIEAPEGWIYKVK
EEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure 
SKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGL
CCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDRNAARIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTG
HHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGMAPTNFIVSDFDRAAPNGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCC
AAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITNDDKFITPYSP
CEECCCCEEECCCCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCCCC
SILPSITKYSLLYLAEHRLGLKAEEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDF
CHHHHHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
HVFHSETEVGPVTKRLFDELCGIQFGDIEAPEGWIYKVK
EEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]