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The map label for this gene is fabI [H]

Identifier: 116872370

GI number: 116872370

Start: 976581

End: 977369

Strand: Direct

Name: fabI [H]

Synonym: lwe0952

Alternate gene names: 116872370

Gene position: 976581-977369 (Clockwise)

Preceding gene: 116872369

Following gene: 116872376

Centisome position: 34.7

GC content: 38.4

Gene sequence:

>789_bases
ATGAATTTATCATTAGAGGGAAAAACATATGTTGTAATGGGAGTTGCTAATAAGCGTAGTATTGCTTGGGCGATTGCGCG
TTCATTAAATGAAGCAGGAGCAAAACTAGTATTTACATATGCCGATGATCGGGCGAAAAAAAGTATTACTGAACTTGTAC
CATCATTAAACGAAATAAATCAAGAACCACTTATTCTCGCATGTGATGTAACGAGTGAGGAGGCTATTACAGCAACATTT
GAAACAATTAAAAATGAAGTAGGTCAACTTAGCGGTTTAGCACATTGTATTGCATTTGCTAATAAAGATTACTTAACGGG
TGATTATTTAGAAGTAGATCGCAAAAGCTTTTTACAAGCGCATGAAATTAGTGCATACTCGTTTACAGCGGTGGCTCGGG
CGTTAAAACATTTAGAAATGCTAACAGAAGACGCAAGTTTACTAACGCTGACTTATCTTGGTGGTGAACGTGTGGTAGAA
AACTATAACATTATGGGTGTTGCGAAAGCTTCTCTTGATGCAAGTGTTAGATACCTTGCAATGGACTTAGGAGCTATTGG
TGTTCGTGTCAATGCTATTTCTGCTGGACCAATTAGAACAGTTTCTGCGCGTGGTGTTAGTGGCTTCTCTGATTCCATCT
CCCTTGTAGAAGAAAGAGCACCTCTAAAACGTGCTACTCAAGCGGAAGAAGTTGGAGATACTGCTTATTACTTATTCAGT
AATTTATCTAGGGGCGTTACAGGTGAAGTAATTCATGTGGATAGCGGTTATCATATTATTGGATTCTAA

Upstream 100 bases:

>100_bases
AGATAATTCAAAAATCGGAGTAATAAGAGTTTGAAAAAGTTATTTGGTCATGTAAAATGAAAGAAGCGAACAAATCGCAT
CAATTTTAGGAGGAAAGAAC

Downstream 100 bases:

>100_bases
TAAGAATTAAAAAAATCCAAAAGTAGTCTTAAAACTACTTTTGGATTTTTTTGTGTTCTTGTTTAGGATGATTTTTCATA
TAAAATTCATCTGCTGTTGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNLSLEGKTYVVMGVANKRSIAWAIARSLNEAGAKLVFTYADDRAKKSITELVPSLNEINQEPLILACDVTSEEAITATF
ETIKNEVGQLSGLAHCIAFANKDYLTGDYLEVDRKSFLQAHEISAYSFTAVARALKHLEMLTEDASLLTLTYLGGERVVE
NYNIMGVAKASLDASVRYLAMDLGAIGVRVNAISAGPIRTVSARGVSGFSDSISLVEERAPLKRATQAEEVGDTAYYLFS
NLSRGVTGEVIHVDSGYHIIGF

Sequences:

>Translated_262_residues
MNLSLEGKTYVVMGVANKRSIAWAIARSLNEAGAKLVFTYADDRAKKSITELVPSLNEINQEPLILACDVTSEEAITATF
ETIKNEVGQLSGLAHCIAFANKDYLTGDYLEVDRKSFLQAHEISAYSFTAVARALKHLEMLTEDASLLTLTYLGGERVVE
NYNIMGVAKASLDASVRYLAMDLGAIGVRVNAISAGPIRTVSARGVSGFSDSISLVEERAPLKRATQAEEVGDTAYYLFS
NLSRGVTGEVIHVDSGYHIIGF
>Mature_262_residues
MNLSLEGKTYVVMGVANKRSIAWAIARSLNEAGAKLVFTYADDRAKKSITELVPSLNEINQEPLILACDVTSEEAITATF
ETIKNEVGQLSGLAHCIAFANKDYLTGDYLEVDRKSFLQAHEISAYSFTAVARALKHLEMLTEDASLLTLTYLGGERVVE
NYNIMGVAKASLDASVRYLAMDLGAIGVRVNAISAGPIRTVSARGVSGFSDSISLVEERAPLKRATQAEEVGDTAYYLFS
NLSRGVTGEVIHVDSGYHIIGF

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=256, Percent_Identity=45.3125, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=26.3358778625954, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=25.7936507936508, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28337; Mature: 28337

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSLEGKTYVVMGVANKRSIAWAIARSLNEAGAKLVFTYADDRAKKSITELVPSLNEIN
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCC
QEPLILACDVTSEEAITATFETIKNEVGQLSGLAHCIAFANKDYLTGDYLEVDRKSFLQA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
HEISAYSFTAVARALKHLEMLTEDASLLTLTYLGGERVVENYNIMGVAKASLDASVRYLA
HHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEEEEECCCCHHHHEEE
MDLGAIGVRVNAISAGPIRTVSARGVSGFSDSISLVEERAPLKRATQAEEVGDTAYYLFS
EECCEEEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
NLSRGVTGEVIHVDSGYHIIGF
HCCCCCCCEEEEECCCEEEEEC
>Mature Secondary Structure
MNLSLEGKTYVVMGVANKRSIAWAIARSLNEAGAKLVFTYADDRAKKSITELVPSLNEIN
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCC
QEPLILACDVTSEEAITATFETIKNEVGQLSGLAHCIAFANKDYLTGDYLEVDRKSFLQA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
HEISAYSFTAVARALKHLEMLTEDASLLTLTYLGGERVVENYNIMGVAKASLDASVRYLA
HHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEEEEECCCCHHHHEEE
MDLGAIGVRVNAISAGPIRTVSARGVSGFSDSISLVEERAPLKRATQAEEVGDTAYYLFS
EECCEEEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
NLSRGVTGEVIHVDSGYHIIGF
HCCCCCCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]